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A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement.

Streckfus CF, Storthz KA, Bigler L, Dubinsky WP - J Oncol (2009)

Bottom Line: Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer.Results.The results yielded approximately 174 differentially expressed proteins in the saliva specimens.

View Article: PubMed Central - PubMed

Affiliation: UTHSC-Dental Branch, CCTS-Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USA.

ABSTRACT
Purpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb. The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer. Results. The results yielded approximately 174 differentially expressed proteins in the saliva specimens. There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb.

No MeSH data available.


Related in: MedlinePlus

Differential expressions of salivary proteins that were not common to both stage IIa and stage IIb.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig4: Differential expressions of salivary proteins that were not common to both stage IIa and stage IIb.

Mentions: Figure 2 represents a Venn diagram of the overlapping proteins between the three groups of women. Figure 3 illustrates the comparison of the log ratio of the relative intensity (cancer/control) of the proteins which were common to both Stage IIa and Stage IIb while Figure 4 shows the proteins that were different between the two groups. It is worth noting that in Figure 3 the stage IIb protein ratios (; ±0.471) are greater than the stage IIa ratios (; ±0.469) for the same proteins. Consequently, a paired t-test was performed comparing the two groups of values. The difference in the mean values between the two groups is greater than would be expected by chance; there is a statistically significant difference (t = −2.882;  P < .008).


A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement.

Streckfus CF, Storthz KA, Bigler L, Dubinsky WP - J Oncol (2009)

Differential expressions of salivary proteins that were not common to both stage IIa and stage IIb.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2801014&req=5

fig4: Differential expressions of salivary proteins that were not common to both stage IIa and stage IIb.
Mentions: Figure 2 represents a Venn diagram of the overlapping proteins between the three groups of women. Figure 3 illustrates the comparison of the log ratio of the relative intensity (cancer/control) of the proteins which were common to both Stage IIa and Stage IIb while Figure 4 shows the proteins that were different between the two groups. It is worth noting that in Figure 3 the stage IIb protein ratios (; ±0.471) are greater than the stage IIa ratios (; ±0.469) for the same proteins. Consequently, a paired t-test was performed comparing the two groups of values. The difference in the mean values between the two groups is greater than would be expected by chance; there is a statistically significant difference (t = −2.882;  P < .008).

Bottom Line: Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer.Results.The results yielded approximately 174 differentially expressed proteins in the saliva specimens.

View Article: PubMed Central - PubMed

Affiliation: UTHSC-Dental Branch, CCTS-Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USA.

ABSTRACT
Purpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb. The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer. Results. The results yielded approximately 174 differentially expressed proteins in the saliva specimens. There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb.

No MeSH data available.


Related in: MedlinePlus