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Effect of NMSO3 treatment in a murine model of human metapneumovirus infection.

Spetch L, Bowlin TL, Casola A - J. Gen. Virol. (2008)

Bottom Line: BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection.NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.

ABSTRACT
BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection. NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

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Related in: MedlinePlus

Effect of NMSO3 on cytokine and chemokine release in BAL. Cytokine and chemokine production were measured using a Bioplex array in the BAL of mock- and hMPV-infected mice, either untreated or treated with NMSO3, at days 1 (a, b) and 4 (c) p.i. Data are expressed as means±sd and are representative of three different experiments. *, P<0.01 relative to hMPV-infected mice.
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f3: Effect of NMSO3 on cytokine and chemokine release in BAL. Cytokine and chemokine production were measured using a Bioplex array in the BAL of mock- and hMPV-infected mice, either untreated or treated with NMSO3, at days 1 (a, b) and 4 (c) p.i. Data are expressed as means±sd and are representative of three different experiments. *, P<0.01 relative to hMPV-infected mice.

Mentions: HMPV infection of BALB/c mice results in significant production of cytokines and chemokines (Alvarez et al., 2004; Guerrero-Plata et al., 2005; Hamelin et al., 2005), which are likely to play a major role in pulmonary inflammation. Therefore, we determined whether NMSO3 treatment could modulate hMPV-induced cytokine and chemokine secretion by analysing their levels in BAL samples collected at days 1 and 4 p.i. As shown in Fig. 3(a), NMSO3 treatment significantly reduced the level of the chemokine RANTES, a potent chemoattractant of mononuclear cells, while increasing the amount of KC, a chemokine important for neutrophil recruitment. The pro-inflammatory cytokines interleukin (IL)-1α, IL-1β, IL-6 and tumour necrosis factor alpha were strongly upregulated in the lungs of infected animals at day 1 p.i., and NMSO3 treatment significantly reduced all four cytokine protein levels (Fig. 3b). These cytokines were no longer detectable in the BALs of infected mice at day 4 p.i. In contrast, granulocyte colony-stimulating factor (G-CSF) and gamma interferon (IFN-γ) were secreted in significant amounts at day 4 p.i., and were significantly reduced in hMPV-infected animals treated with NMSO3 (Fig. 3c).


Effect of NMSO3 treatment in a murine model of human metapneumovirus infection.

Spetch L, Bowlin TL, Casola A - J. Gen. Virol. (2008)

Effect of NMSO3 on cytokine and chemokine release in BAL. Cytokine and chemokine production were measured using a Bioplex array in the BAL of mock- and hMPV-infected mice, either untreated or treated with NMSO3, at days 1 (a, b) and 4 (c) p.i. Data are expressed as means±sd and are representative of three different experiments. *, P<0.01 relative to hMPV-infected mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2800786&req=5

f3: Effect of NMSO3 on cytokine and chemokine release in BAL. Cytokine and chemokine production were measured using a Bioplex array in the BAL of mock- and hMPV-infected mice, either untreated or treated with NMSO3, at days 1 (a, b) and 4 (c) p.i. Data are expressed as means±sd and are representative of three different experiments. *, P<0.01 relative to hMPV-infected mice.
Mentions: HMPV infection of BALB/c mice results in significant production of cytokines and chemokines (Alvarez et al., 2004; Guerrero-Plata et al., 2005; Hamelin et al., 2005), which are likely to play a major role in pulmonary inflammation. Therefore, we determined whether NMSO3 treatment could modulate hMPV-induced cytokine and chemokine secretion by analysing their levels in BAL samples collected at days 1 and 4 p.i. As shown in Fig. 3(a), NMSO3 treatment significantly reduced the level of the chemokine RANTES, a potent chemoattractant of mononuclear cells, while increasing the amount of KC, a chemokine important for neutrophil recruitment. The pro-inflammatory cytokines interleukin (IL)-1α, IL-1β, IL-6 and tumour necrosis factor alpha were strongly upregulated in the lungs of infected animals at day 1 p.i., and NMSO3 treatment significantly reduced all four cytokine protein levels (Fig. 3b). These cytokines were no longer detectable in the BALs of infected mice at day 4 p.i. In contrast, granulocyte colony-stimulating factor (G-CSF) and gamma interferon (IFN-γ) were secreted in significant amounts at day 4 p.i., and were significantly reduced in hMPV-infected animals treated with NMSO3 (Fig. 3c).

Bottom Line: BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection.NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.

ABSTRACT
BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection. NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

Show MeSH
Related in: MedlinePlus