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Effect of NMSO3 treatment in a murine model of human metapneumovirus infection.

Spetch L, Bowlin TL, Casola A - J. Gen. Virol. (2008)

Bottom Line: BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection.NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.

ABSTRACT
BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection. NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

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Effect of NMSO3 on airway inflammation. (a) The total number of cells was measured in the BAL of hMPV-infected mice, either untreated or treated with NMSO3, at days 1 and 4 p.i. Data are expressed as means±sd and are representative of three different experiments. (b) Differential cell count was measured in BALs of hMPV-infected mice, untreated or treated with NMSO3, at days 1 and 4 p.i. *, P<0.01 relative to hMPV-infected mice.
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f2: Effect of NMSO3 on airway inflammation. (a) The total number of cells was measured in the BAL of hMPV-infected mice, either untreated or treated with NMSO3, at days 1 and 4 p.i. Data are expressed as means±sd and are representative of three different experiments. (b) Differential cell count was measured in BALs of hMPV-infected mice, untreated or treated with NMSO3, at days 1 and 4 p.i. *, P<0.01 relative to hMPV-infected mice.

Mentions: To determine whether NMSO3 altered hMPV-induced lung inflammation, total and differential cell counts in bronchoalveolar lavages (BALs) were measured. In mock-infected mice, total lung cell count was between 1×104 and 1.5×104, with macrophages representing the majority of the cell population. We observed a significant attenuation of total cellular influx following NMSO3 treatment in HMPV-infected mice on day 1 p.i. (P=0.056), with a similar trend at day 4 p.i. (Fig. 2a). HMPV infection induces a significant recruitment of neutrophils to the lung within the first 3 days of infection, whilst mononuclear cells, including macrophages/monocytes and lymphocytes, start to increase at day 3 p.i., representing the majority of the lung inflammatory cells from day 5 p.i. (Kolli et al., 2008). NMSO3 administration did not cause significant changes in the distribution of the inflammatory cell population at day 1 p.i. between treated and untreated groups, whilst there was a significant increase in neutrophil recruitment in the airways by day 4 p.i. (Fig. 2b).


Effect of NMSO3 treatment in a murine model of human metapneumovirus infection.

Spetch L, Bowlin TL, Casola A - J. Gen. Virol. (2008)

Effect of NMSO3 on airway inflammation. (a) The total number of cells was measured in the BAL of hMPV-infected mice, either untreated or treated with NMSO3, at days 1 and 4 p.i. Data are expressed as means±sd and are representative of three different experiments. (b) Differential cell count was measured in BALs of hMPV-infected mice, untreated or treated with NMSO3, at days 1 and 4 p.i. *, P<0.01 relative to hMPV-infected mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC2800786&req=5

f2: Effect of NMSO3 on airway inflammation. (a) The total number of cells was measured in the BAL of hMPV-infected mice, either untreated or treated with NMSO3, at days 1 and 4 p.i. Data are expressed as means±sd and are representative of three different experiments. (b) Differential cell count was measured in BALs of hMPV-infected mice, untreated or treated with NMSO3, at days 1 and 4 p.i. *, P<0.01 relative to hMPV-infected mice.
Mentions: To determine whether NMSO3 altered hMPV-induced lung inflammation, total and differential cell counts in bronchoalveolar lavages (BALs) were measured. In mock-infected mice, total lung cell count was between 1×104 and 1.5×104, with macrophages representing the majority of the cell population. We observed a significant attenuation of total cellular influx following NMSO3 treatment in HMPV-infected mice on day 1 p.i. (P=0.056), with a similar trend at day 4 p.i. (Fig. 2a). HMPV infection induces a significant recruitment of neutrophils to the lung within the first 3 days of infection, whilst mononuclear cells, including macrophages/monocytes and lymphocytes, start to increase at day 3 p.i., representing the majority of the lung inflammatory cells from day 5 p.i. (Kolli et al., 2008). NMSO3 administration did not cause significant changes in the distribution of the inflammatory cell population at day 1 p.i. between treated and untreated groups, whilst there was a significant increase in neutrophil recruitment in the airways by day 4 p.i. (Fig. 2b).

Bottom Line: BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection.NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.

ABSTRACT
BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection. NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hMPV-induced disease.

Show MeSH
Related in: MedlinePlus