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Real time in vitro studies of doxorubicin release from PHEMA nanoparticles.

Chouhan R, Bajpai A - J Nanobiotechnology (2009)

Bottom Line: Many anticancer agents have poor water solubility and therefore the development of novel delivery systems for such molecules has received significant attention.PHEMA nanoparticles have been synthesized and characterized using FTIR and scanning electron microscopy (SEM), particle size analysis and surface charge measurements.PHEMA nanoparticles can potentially be used for the controlled release of the anticancer drug doxorubicin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Bose Memorial Research Laboratory, Department of Chemistry, Government Autonomous Science College, Jabalpur (MP)-482001, India. akbmrl@yahoo.co.in.

ABSTRACT

Background: Many anticancer agents have poor water solubility and therefore the development of novel delivery systems for such molecules has received significant attention. Nanocarriers show great potential in delivering therapeutic agents into the targeted organs or cells and have recently emerged as a promising approach to cancer treatments. The aim of this study was to prepare and use poly-2-hydroxyethyl methacrylate (PHEMA) nanoparticles for the controlled release of the anticancer drug doxorubicin.

Results: PHEMA nanoparticles have been synthesized and characterized using FTIR and scanning electron microscopy (SEM), particle size analysis and surface charge measurements. We also studied the effects of various parameters such as percent loading of drugs, chemical architecture of the nanocarriers, pH, temperature and nature of the release media on the release profiles of the drug. The chemical stability of doxorubicin in PBS was assessed at a range of pH.

Conclusion: Suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) results in the formation of swellable nanoparticles of defined composition. PHEMA nanoparticles can potentially be used for the controlled release of the anticancer drug doxorubicin.

No MeSH data available.


Related in: MedlinePlus

Effect of initiator [Bz2O2]. Effect of initiator [Bz2O2] content on the (a) swelling profile and (b) release profile of the nanoparticles of definite composition, [HEMA] = 12.37 mM, [EGDMA] = 1.06 mM, % loading = 28%, pH = 7.4, temp. = 37°C.
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Figure 7: Effect of initiator [Bz2O2]. Effect of initiator [Bz2O2] content on the (a) swelling profile and (b) release profile of the nanoparticles of definite composition, [HEMA] = 12.37 mM, [EGDMA] = 1.06 mM, % loading = 28%, pH = 7.4, temp. = 37°C.

Mentions: In free radical polymerization the concentration of initiator has a direct impact on the molecular weight of the polymer [31]. We used Bz2O2 as a polymerization initiator and its concentration in the reaction mixture was varied in the range of 0.082 - 0.330 mM. Swelling and release results are depicted in Fig. 7(a) and 7(b). An initial increase in concentration of Bz2O2 in the range of 0.082 - 0.248 mM results in an increased swelling as well as drug release. The increase in the concentration of initiator may bring about an increase in the number of primary free radicals, which may eventually result in lower molecular weight of the PHEMA. Since a polymer with lower molecular weight has lower hydrodynamic volume in aqueous solution, the PHEMA chains acquire greater mobility and, therefore, show increased swelling and increased drug release. However, the higher concentration of the initiator results in shorter PHEMA chains and smaller mesh size of the polymer network and reduced drug loading and release.


Real time in vitro studies of doxorubicin release from PHEMA nanoparticles.

Chouhan R, Bajpai A - J Nanobiotechnology (2009)

Effect of initiator [Bz2O2]. Effect of initiator [Bz2O2] content on the (a) swelling profile and (b) release profile of the nanoparticles of definite composition, [HEMA] = 12.37 mM, [EGDMA] = 1.06 mM, % loading = 28%, pH = 7.4, temp. = 37°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2770983&req=5

Figure 7: Effect of initiator [Bz2O2]. Effect of initiator [Bz2O2] content on the (a) swelling profile and (b) release profile of the nanoparticles of definite composition, [HEMA] = 12.37 mM, [EGDMA] = 1.06 mM, % loading = 28%, pH = 7.4, temp. = 37°C.
Mentions: In free radical polymerization the concentration of initiator has a direct impact on the molecular weight of the polymer [31]. We used Bz2O2 as a polymerization initiator and its concentration in the reaction mixture was varied in the range of 0.082 - 0.330 mM. Swelling and release results are depicted in Fig. 7(a) and 7(b). An initial increase in concentration of Bz2O2 in the range of 0.082 - 0.248 mM results in an increased swelling as well as drug release. The increase in the concentration of initiator may bring about an increase in the number of primary free radicals, which may eventually result in lower molecular weight of the PHEMA. Since a polymer with lower molecular weight has lower hydrodynamic volume in aqueous solution, the PHEMA chains acquire greater mobility and, therefore, show increased swelling and increased drug release. However, the higher concentration of the initiator results in shorter PHEMA chains and smaller mesh size of the polymer network and reduced drug loading and release.

Bottom Line: Many anticancer agents have poor water solubility and therefore the development of novel delivery systems for such molecules has received significant attention.PHEMA nanoparticles have been synthesized and characterized using FTIR and scanning electron microscopy (SEM), particle size analysis and surface charge measurements.PHEMA nanoparticles can potentially be used for the controlled release of the anticancer drug doxorubicin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Bose Memorial Research Laboratory, Department of Chemistry, Government Autonomous Science College, Jabalpur (MP)-482001, India. akbmrl@yahoo.co.in.

ABSTRACT

Background: Many anticancer agents have poor water solubility and therefore the development of novel delivery systems for such molecules has received significant attention. Nanocarriers show great potential in delivering therapeutic agents into the targeted organs or cells and have recently emerged as a promising approach to cancer treatments. The aim of this study was to prepare and use poly-2-hydroxyethyl methacrylate (PHEMA) nanoparticles for the controlled release of the anticancer drug doxorubicin.

Results: PHEMA nanoparticles have been synthesized and characterized using FTIR and scanning electron microscopy (SEM), particle size analysis and surface charge measurements. We also studied the effects of various parameters such as percent loading of drugs, chemical architecture of the nanocarriers, pH, temperature and nature of the release media on the release profiles of the drug. The chemical stability of doxorubicin in PBS was assessed at a range of pH.

Conclusion: Suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) results in the formation of swellable nanoparticles of defined composition. PHEMA nanoparticles can potentially be used for the controlled release of the anticancer drug doxorubicin.

No MeSH data available.


Related in: MedlinePlus