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Generating chimeric zebrafish embryos by transplantation.

Kemp HA, Carmany-Rampey A, Moens C - J Vis Exp (2009)

Bottom Line: One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos.Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo.Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope.

View Article: PubMed Central - PubMed

Affiliation: HHMI and Division of Basic Sciences, Fred Hutchinson Cancer Research Center.

ABSTRACT
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an organism that contains cells from more than one animal; mosaics are one type of chimera in which cells from more than one genotype are mixed, usually wild-type and mutant. In the zebrafish, chimeras can be readily made by transplantation of cells from a donor embryo into a host embryo at the appropriate embryonic stage. Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo. Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope. Donor cells can in some cases be targeted to a specific region or tissue of the developing blastula or gastrula stage host embryo by choosing a transplantation site in the host embryo based on well-established fate maps.

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Related in: MedlinePlus

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Generating chimeric zebrafish embryos by transplantation.

Kemp HA, Carmany-Rampey A, Moens C - J Vis Exp (2009)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2770904&req=5

Bottom Line: One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos.Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo.Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope.

View Article: PubMed Central - PubMed

Affiliation: HHMI and Division of Basic Sciences, Fred Hutchinson Cancer Research Center.

ABSTRACT
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an organism that contains cells from more than one animal; mosaics are one type of chimera in which cells from more than one genotype are mixed, usually wild-type and mutant. In the zebrafish, chimeras can be readily made by transplantation of cells from a donor embryo into a host embryo at the appropriate embryonic stage. Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo. Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope. Donor cells can in some cases be targeted to a specific region or tissue of the developing blastula or gastrula stage host embryo by choosing a transplantation site in the host embryo based on well-established fate maps.

Show MeSH
Related in: MedlinePlus