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The effect of amnion-derived cellular cytokine solution on the epithelialization of partial-thickness donor site wounds in normal and streptozotocin-induced diabetic swine.

Bergmann J, Hackl F, Koyama T, Aflaki P, Smith CA, Robson MC, Eriksson E - Eplasty (2009)

Bottom Line: Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12.Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds.

View Article: PubMed Central - PubMed

Affiliation: Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

ABSTRACT

Objective: The purpose of this study was to determine whether amnion-derived cellular cytokine solution (ACCS) could improve the quality of epithelialization and accelerate closure of dermatome-created partial-thickness wounds in normal and streptozotocin-induced diabetic pigs.

Methods: Dermatome-created partial-thickness wounds were sealed with wound chambers in healthy and diabetic pigs and were injected with ACCS. Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12. Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.

Results: The macroscopic appearance of the wounds and speed of healing was similar in all groups at each time point. All wounds were healed by day 6. The epidermis was thicker in the ACCS-treated diabetic wounds than in the controls (140.6 microm vs 82.7 microm on day 12 in diabetic pigs). There were more cell layers (13 vs 7.7) in ACCS-treated diabetic pigs on day 12. The number of rete ridges per 2.5 mm was greater on day 12 in the ACCS-treated diabetic wounds (13 vs 8). There was also a significant increase in the number of rete ridges in ACCS-treated nondiabetic pigs but no difference in epidermal thickness or number of cell layers.

Conclusion: In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds. Healthy pigs showed more rete ridges but no difference in thickness of epidermis or number of cell layers on day 12.

No MeSH data available.


Related in: MedlinePlus

Number of rete ridges per 2.5 mm on biopsy days 6, 8, 10, and 12 in diabetic pigs (A: 0.455-mL ACCS/cm2, B: 0.255-mL ACCS/cm2, D: 0.111-mL ACCS/cm2, E: 0.045-mL ACCS/cm2, F: 0.022-mL ACCS/cm2, C: control).
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Figure 8: Number of rete ridges per 2.5 mm on biopsy days 6, 8, 10, and 12 in diabetic pigs (A: 0.455-mL ACCS/cm2, B: 0.255-mL ACCS/cm2, D: 0.111-mL ACCS/cm2, E: 0.045-mL ACCS/cm2, F: 0.022-mL ACCS/cm2, C: control).

Mentions: Summarizing the histological data, including thickness of the epithelium, number of cell layers in the newly formed epithelium, and rete formation, we recognized numeric differences. More epidermal cell layers and thicker epidermis were found at all concentrations of ACCS compared with the saline control (Figs 5–8). Moreover, the rete formation in the ACCS groups was higher than in the control.


The effect of amnion-derived cellular cytokine solution on the epithelialization of partial-thickness donor site wounds in normal and streptozotocin-induced diabetic swine.

Bergmann J, Hackl F, Koyama T, Aflaki P, Smith CA, Robson MC, Eriksson E - Eplasty (2009)

Number of rete ridges per 2.5 mm on biopsy days 6, 8, 10, and 12 in diabetic pigs (A: 0.455-mL ACCS/cm2, B: 0.255-mL ACCS/cm2, D: 0.111-mL ACCS/cm2, E: 0.045-mL ACCS/cm2, F: 0.022-mL ACCS/cm2, C: control).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2770806&req=5

Figure 8: Number of rete ridges per 2.5 mm on biopsy days 6, 8, 10, and 12 in diabetic pigs (A: 0.455-mL ACCS/cm2, B: 0.255-mL ACCS/cm2, D: 0.111-mL ACCS/cm2, E: 0.045-mL ACCS/cm2, F: 0.022-mL ACCS/cm2, C: control).
Mentions: Summarizing the histological data, including thickness of the epithelium, number of cell layers in the newly formed epithelium, and rete formation, we recognized numeric differences. More epidermal cell layers and thicker epidermis were found at all concentrations of ACCS compared with the saline control (Figs 5–8). Moreover, the rete formation in the ACCS groups was higher than in the control.

Bottom Line: Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12.Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds.

View Article: PubMed Central - PubMed

Affiliation: Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

ABSTRACT

Objective: The purpose of this study was to determine whether amnion-derived cellular cytokine solution (ACCS) could improve the quality of epithelialization and accelerate closure of dermatome-created partial-thickness wounds in normal and streptozotocin-induced diabetic pigs.

Methods: Dermatome-created partial-thickness wounds were sealed with wound chambers in healthy and diabetic pigs and were injected with ACCS. Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12. Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.

Results: The macroscopic appearance of the wounds and speed of healing was similar in all groups at each time point. All wounds were healed by day 6. The epidermis was thicker in the ACCS-treated diabetic wounds than in the controls (140.6 microm vs 82.7 microm on day 12 in diabetic pigs). There were more cell layers (13 vs 7.7) in ACCS-treated diabetic pigs on day 12. The number of rete ridges per 2.5 mm was greater on day 12 in the ACCS-treated diabetic wounds (13 vs 8). There was also a significant increase in the number of rete ridges in ACCS-treated nondiabetic pigs but no difference in epidermal thickness or number of cell layers.

Conclusion: In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds. Healthy pigs showed more rete ridges but no difference in thickness of epidermis or number of cell layers on day 12.

No MeSH data available.


Related in: MedlinePlus