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The effect of amnion-derived cellular cytokine solution on the epithelialization of partial-thickness donor site wounds in normal and streptozotocin-induced diabetic swine.

Bergmann J, Hackl F, Koyama T, Aflaki P, Smith CA, Robson MC, Eriksson E - Eplasty (2009)

Bottom Line: Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12.Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds.

View Article: PubMed Central - PubMed

Affiliation: Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

ABSTRACT

Objective: The purpose of this study was to determine whether amnion-derived cellular cytokine solution (ACCS) could improve the quality of epithelialization and accelerate closure of dermatome-created partial-thickness wounds in normal and streptozotocin-induced diabetic pigs.

Methods: Dermatome-created partial-thickness wounds were sealed with wound chambers in healthy and diabetic pigs and were injected with ACCS. Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12. Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.

Results: The macroscopic appearance of the wounds and speed of healing was similar in all groups at each time point. All wounds were healed by day 6. The epidermis was thicker in the ACCS-treated diabetic wounds than in the controls (140.6 microm vs 82.7 microm on day 12 in diabetic pigs). There were more cell layers (13 vs 7.7) in ACCS-treated diabetic pigs on day 12. The number of rete ridges per 2.5 mm was greater on day 12 in the ACCS-treated diabetic wounds (13 vs 8). There was also a significant increase in the number of rete ridges in ACCS-treated nondiabetic pigs but no difference in epidermal thickness or number of cell layers.

Conclusion: In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds. Healthy pigs showed more rete ridges but no difference in thickness of epidermis or number of cell layers on day 12.

No MeSH data available.


Related in: MedlinePlus

Macroscopic pictures of representative wounds on days 6 and 10 in diabetic pigs: (a) day 6, 0.455-mL ACCS/cm2; (b) day 6, control; (c) day 10, 0.455-mL ACCS/cm2; and (d) day 10, control. ACCS indicates amnion-derived cellular cytokine solution.
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Figure 10: Macroscopic pictures of representative wounds on days 6 and 10 in diabetic pigs: (a) day 6, 0.455-mL ACCS/cm2; (b) day 6, control; (c) day 10, 0.455-mL ACCS/cm2; and (d) day 10, control. ACCS indicates amnion-derived cellular cytokine solution.

Mentions: Cells in culture secrete factors that may provide support to or affect the growth, differentiation, and protein production of other cells.9 Stem cells and stem cell–like multipotent cells are known to produce cytokine growth factors that serve as mediators to the cellular processes of the wound-healing scheme.3 Normal wound healing is accomplished by a combination of cytokines, and they occur in a “natural” cascade.11 ACCS has been shown to have cytokines at physiological levels including PDGF, VEGF, angiogenin, TGF-β2, TIMP-1, and TIMP-2.9 Applying ACCS in a continuous “wet” environment accelerated and improved healing in the 2 experiments in both normal and diabetic swine. Maturation of the healed epidermis was accelerated both macroscopically (Fig 10) and microscopically.


The effect of amnion-derived cellular cytokine solution on the epithelialization of partial-thickness donor site wounds in normal and streptozotocin-induced diabetic swine.

Bergmann J, Hackl F, Koyama T, Aflaki P, Smith CA, Robson MC, Eriksson E - Eplasty (2009)

Macroscopic pictures of representative wounds on days 6 and 10 in diabetic pigs: (a) day 6, 0.455-mL ACCS/cm2; (b) day 6, control; (c) day 10, 0.455-mL ACCS/cm2; and (d) day 10, control. ACCS indicates amnion-derived cellular cytokine solution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2770806&req=5

Figure 10: Macroscopic pictures of representative wounds on days 6 and 10 in diabetic pigs: (a) day 6, 0.455-mL ACCS/cm2; (b) day 6, control; (c) day 10, 0.455-mL ACCS/cm2; and (d) day 10, control. ACCS indicates amnion-derived cellular cytokine solution.
Mentions: Cells in culture secrete factors that may provide support to or affect the growth, differentiation, and protein production of other cells.9 Stem cells and stem cell–like multipotent cells are known to produce cytokine growth factors that serve as mediators to the cellular processes of the wound-healing scheme.3 Normal wound healing is accomplished by a combination of cytokines, and they occur in a “natural” cascade.11 ACCS has been shown to have cytokines at physiological levels including PDGF, VEGF, angiogenin, TGF-β2, TIMP-1, and TIMP-2.9 Applying ACCS in a continuous “wet” environment accelerated and improved healing in the 2 experiments in both normal and diabetic swine. Maturation of the healed epidermis was accelerated both macroscopically (Fig 10) and microscopically.

Bottom Line: Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12.Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds.

View Article: PubMed Central - PubMed

Affiliation: Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

ABSTRACT

Objective: The purpose of this study was to determine whether amnion-derived cellular cytokine solution (ACCS) could improve the quality of epithelialization and accelerate closure of dermatome-created partial-thickness wounds in normal and streptozotocin-induced diabetic pigs.

Methods: Dermatome-created partial-thickness wounds were sealed with wound chambers in healthy and diabetic pigs and were injected with ACCS. Wound fluid was exchanged daily for total protein concentration, and biopsies were taken on days 6, 8, 10, and 12. Epithelialization, thickness of epidermis, number of epidermal cell layers, and rete ridges were evaluated.

Results: The macroscopic appearance of the wounds and speed of healing was similar in all groups at each time point. All wounds were healed by day 6. The epidermis was thicker in the ACCS-treated diabetic wounds than in the controls (140.6 microm vs 82.7 microm on day 12 in diabetic pigs). There were more cell layers (13 vs 7.7) in ACCS-treated diabetic pigs on day 12. The number of rete ridges per 2.5 mm was greater on day 12 in the ACCS-treated diabetic wounds (13 vs 8). There was also a significant increase in the number of rete ridges in ACCS-treated nondiabetic pigs but no difference in epidermal thickness or number of cell layers.

Conclusion: In diabetic pigs, we found a significantly thicker epidermis and more cell layers and rete ridges in the ACCS-treated wounds. Healthy pigs showed more rete ridges but no difference in thickness of epidermis or number of cell layers on day 12.

No MeSH data available.


Related in: MedlinePlus