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Stimulation of lymphocyte anti-melanoma activity by co-cultured macrophages activated by complex homeopathic medication.

Guimarães FS, Abud AP, Oliveira SM, Oliveira CC, César B, Andrade LF, Donatti L, Gabardo J, Trindade ES, Buchi DF - BMC Cancer (2009)

Bottom Line: A Brazilian complex homeopathic medication (CHM), used as an immune modulator, has been recommended for patients with depressed immune systems.Previous studies in mice have demonstrated that the CHM activates macrophages, induces an increase in the number of leukocytes and improves the murine response against Sarcoma-180.In addition, they are attractive combination-therapy candidates, which may enhance the efficacy of conventional medicines by improving the immune response against tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Biologia Celular, Laboratório de Pesquisa em Células Inflamatórias e Neoplásicas, Universidade Federal do Paraná (UFPR), Curitiba - PR, Brazil. ferbiocel@yahoo.com.br

ABSTRACT

Background: Melanoma is the most aggressive form of skin cancer, and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have been uniformly disappointing. A Brazilian complex homeopathic medication (CHM), used as an immune modulator, has been recommended for patients with depressed immune systems. Previous studies in mice have demonstrated that the CHM activates macrophages, induces an increase in the number of leukocytes and improves the murine response against Sarcoma-180.

Methods: Here we studied the interaction of mouse lymph node lymphocytes, co-cultured in vitro with macrophages in the presence or absence of the CHM, with B16F10 melanoma cells.

Results: Lymphocytes co-cultured with macrophages in the presence of the CHM had greater anti-melanoma activity, reducing melanoma cell density and increasing the number of lysed tumor cells. There was also a higher proportion of activated (CD25+) lymphocytes with increased viability. Overall, lymphocytes activated by treatment destroyed growing cancer cells more effectively than control lymphocytes.

Conclusion: Co-culture of macrophages with lymphocytes in the presence of the CHM enhanced the anti-cancer performance of lymphocytes against a very aggressive lineage of melanoma cells. These results suggest that non-toxic therapies using CHMs are a promising alternative approach to the treatment of melanomas. In addition, they are attractive combination-therapy candidates, which may enhance the efficacy of conventional medicines by improving the immune response against tumor cells.

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Micrographs of B16F10 cell density after B16F10/Ly-Mϕ culture condition of control and treated cells respectively (A and B). Cell density was evaluated by cell area selection by ImageJ software and posterior pixel area quantification and comparison, showing a decrease of melanoma cell density on this culture condition (C). Original magnification: 40× objective for all figures.
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Figure 2: Micrographs of B16F10 cell density after B16F10/Ly-Mϕ culture condition of control and treated cells respectively (A and B). Cell density was evaluated by cell area selection by ImageJ software and posterior pixel area quantification and comparison, showing a decrease of melanoma cell density on this culture condition (C). Original magnification: 40× objective for all figures.

Mentions: The morphology of cells in the B16F10/Ly-Mϕ culture condition was dramatically altered by the CHM treatment. Lymphocytes previously in contact with macrophages and treated with the CHM were activated in some manner such that they were capable of destroying B16F10 melanoma cells, resulting in a lower density monolayer of melanoma cells (Fig 1I and 1J). Also the quantified melanoma cell density was significant decreased after treatment (Fig 2A–C).


Stimulation of lymphocyte anti-melanoma activity by co-cultured macrophages activated by complex homeopathic medication.

Guimarães FS, Abud AP, Oliveira SM, Oliveira CC, César B, Andrade LF, Donatti L, Gabardo J, Trindade ES, Buchi DF - BMC Cancer (2009)

Micrographs of B16F10 cell density after B16F10/Ly-Mϕ culture condition of control and treated cells respectively (A and B). Cell density was evaluated by cell area selection by ImageJ software and posterior pixel area quantification and comparison, showing a decrease of melanoma cell density on this culture condition (C). Original magnification: 40× objective for all figures.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2749867&req=5

Figure 2: Micrographs of B16F10 cell density after B16F10/Ly-Mϕ culture condition of control and treated cells respectively (A and B). Cell density was evaluated by cell area selection by ImageJ software and posterior pixel area quantification and comparison, showing a decrease of melanoma cell density on this culture condition (C). Original magnification: 40× objective for all figures.
Mentions: The morphology of cells in the B16F10/Ly-Mϕ culture condition was dramatically altered by the CHM treatment. Lymphocytes previously in contact with macrophages and treated with the CHM were activated in some manner such that they were capable of destroying B16F10 melanoma cells, resulting in a lower density monolayer of melanoma cells (Fig 1I and 1J). Also the quantified melanoma cell density was significant decreased after treatment (Fig 2A–C).

Bottom Line: A Brazilian complex homeopathic medication (CHM), used as an immune modulator, has been recommended for patients with depressed immune systems.Previous studies in mice have demonstrated that the CHM activates macrophages, induces an increase in the number of leukocytes and improves the murine response against Sarcoma-180.In addition, they are attractive combination-therapy candidates, which may enhance the efficacy of conventional medicines by improving the immune response against tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Biologia Celular, Laboratório de Pesquisa em Células Inflamatórias e Neoplásicas, Universidade Federal do Paraná (UFPR), Curitiba - PR, Brazil. ferbiocel@yahoo.com.br

ABSTRACT

Background: Melanoma is the most aggressive form of skin cancer, and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have been uniformly disappointing. A Brazilian complex homeopathic medication (CHM), used as an immune modulator, has been recommended for patients with depressed immune systems. Previous studies in mice have demonstrated that the CHM activates macrophages, induces an increase in the number of leukocytes and improves the murine response against Sarcoma-180.

Methods: Here we studied the interaction of mouse lymph node lymphocytes, co-cultured in vitro with macrophages in the presence or absence of the CHM, with B16F10 melanoma cells.

Results: Lymphocytes co-cultured with macrophages in the presence of the CHM had greater anti-melanoma activity, reducing melanoma cell density and increasing the number of lysed tumor cells. There was also a higher proportion of activated (CD25+) lymphocytes with increased viability. Overall, lymphocytes activated by treatment destroyed growing cancer cells more effectively than control lymphocytes.

Conclusion: Co-culture of macrophages with lymphocytes in the presence of the CHM enhanced the anti-cancer performance of lymphocytes against a very aggressive lineage of melanoma cells. These results suggest that non-toxic therapies using CHMs are a promising alternative approach to the treatment of melanomas. In addition, they are attractive combination-therapy candidates, which may enhance the efficacy of conventional medicines by improving the immune response against tumor cells.

Show MeSH
Related in: MedlinePlus