Limits...
SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells.

Zu X, Yu L, Sun Q, Liu F, Wang J, Xie Z, Wang Y, Xu W, Jiang Y - BMC Res Notes (2009)

Bottom Line: It was found that the GC box within Zbtb7A promoter is necessary for the promoter activity.Furthermore, we identified that Sp1 acts as an activator in the regulation of Zbtb7A promoter activity and the physical interaction between Sp1 and GC box is responsible for the activation of Zbtb7A gene promoter.Our results confirmed that Sp1 upregulates Zbtb7A gene expression via direct binding to GC box within the promoter.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China. zuxuyu0108@hotmail.com

ABSTRACT

Background: Zbtb7A is a proto-oncogenic transcriptional regulator that plays an important role in adipogenesis, osteogenesis and oncogenesis, but little is known about the regulation of Zbtb7A gene expression which is of importance in the function uncovering of this gene.

Finding: Here, a 5'-flanking region of the human Zbtb7A gene was cloned and characterized. It was found that the GC box within Zbtb7A promoter is necessary for the promoter activity. Furthermore, we identified that Sp1 acts as an activator in the regulation of Zbtb7A promoter activity and the physical interaction between Sp1 and GC box is responsible for the activation of Zbtb7A gene promoter.

Conclusion: Our results confirmed that Sp1 upregulates Zbtb7A gene expression via direct binding to GC box within the promoter.

No MeSH data available.


Related in: MedlinePlus

Sp1 increased basal promoter activity could be abrogated by the GC boxes mutation. A, Sp1 enhances the core promoter activity of Zbtb7Agene. HepG-2 and 293 T cells were transient co-tranfected with either pLuc-1000 and pCDNA3.1, or Sp1 and pLuc-10000 or SpAm or SpBm. pGl4.10 was control B, RT-PCR analysis of Zbtb7AmRNA using the total RNA was isolated from 293 T cells tranfected Sp1.GAPDH was control. Data for A means for ± SD from three independent experiments, with each experiment carried out in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2749864&req=5

Figure 3: Sp1 increased basal promoter activity could be abrogated by the GC boxes mutation. A, Sp1 enhances the core promoter activity of Zbtb7Agene. HepG-2 and 293 T cells were transient co-tranfected with either pLuc-1000 and pCDNA3.1, or Sp1 and pLuc-10000 or SpAm or SpBm. pGl4.10 was control B, RT-PCR analysis of Zbtb7AmRNA using the total RNA was isolated from 293 T cells tranfected Sp1.GAPDH was control. Data for A means for ± SD from three independent experiments, with each experiment carried out in triplicate.

Mentions: To further understand the role of Sp1 in Zbtb7Agene transcription, we used 293 T and HePG2 cells as cell model for luciferase reporter assay. Data in Fig. 3A showed that core promoter activity of the Zbtb7Agene increased with the elevated amount of Sp1 expression constructs in 293 T and HePG2 cells, which indicates that basal promoter activity of Zbtb7Agene could be enhanced by the expression of Sp1. Whereas the Sp1 increased basal promoter activity could be abrogated by the mutation of GC boxes which suggestes that the GGGCGG sequence contributes to Sp1 induced activation of basal promoter activity of Zbtb7Agene (Fig. 3A). RT-PCR was used to confirm those results, and as shown in Fig. 3B, Zbtb mRNA was greatly elevated by the over expression of Sp1 in 293 T cells.


SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells.

Zu X, Yu L, Sun Q, Liu F, Wang J, Xie Z, Wang Y, Xu W, Jiang Y - BMC Res Notes (2009)

Sp1 increased basal promoter activity could be abrogated by the GC boxes mutation. A, Sp1 enhances the core promoter activity of Zbtb7Agene. HepG-2 and 293 T cells were transient co-tranfected with either pLuc-1000 and pCDNA3.1, or Sp1 and pLuc-10000 or SpAm or SpBm. pGl4.10 was control B, RT-PCR analysis of Zbtb7AmRNA using the total RNA was isolated from 293 T cells tranfected Sp1.GAPDH was control. Data for A means for ± SD from three independent experiments, with each experiment carried out in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2749864&req=5

Figure 3: Sp1 increased basal promoter activity could be abrogated by the GC boxes mutation. A, Sp1 enhances the core promoter activity of Zbtb7Agene. HepG-2 and 293 T cells were transient co-tranfected with either pLuc-1000 and pCDNA3.1, or Sp1 and pLuc-10000 or SpAm or SpBm. pGl4.10 was control B, RT-PCR analysis of Zbtb7AmRNA using the total RNA was isolated from 293 T cells tranfected Sp1.GAPDH was control. Data for A means for ± SD from three independent experiments, with each experiment carried out in triplicate.
Mentions: To further understand the role of Sp1 in Zbtb7Agene transcription, we used 293 T and HePG2 cells as cell model for luciferase reporter assay. Data in Fig. 3A showed that core promoter activity of the Zbtb7Agene increased with the elevated amount of Sp1 expression constructs in 293 T and HePG2 cells, which indicates that basal promoter activity of Zbtb7Agene could be enhanced by the expression of Sp1. Whereas the Sp1 increased basal promoter activity could be abrogated by the mutation of GC boxes which suggestes that the GGGCGG sequence contributes to Sp1 induced activation of basal promoter activity of Zbtb7Agene (Fig. 3A). RT-PCR was used to confirm those results, and as shown in Fig. 3B, Zbtb mRNA was greatly elevated by the over expression of Sp1 in 293 T cells.

Bottom Line: It was found that the GC box within Zbtb7A promoter is necessary for the promoter activity.Furthermore, we identified that Sp1 acts as an activator in the regulation of Zbtb7A promoter activity and the physical interaction between Sp1 and GC box is responsible for the activation of Zbtb7A gene promoter.Our results confirmed that Sp1 upregulates Zbtb7A gene expression via direct binding to GC box within the promoter.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China. zuxuyu0108@hotmail.com

ABSTRACT

Background: Zbtb7A is a proto-oncogenic transcriptional regulator that plays an important role in adipogenesis, osteogenesis and oncogenesis, but little is known about the regulation of Zbtb7A gene expression which is of importance in the function uncovering of this gene.

Finding: Here, a 5'-flanking region of the human Zbtb7A gene was cloned and characterized. It was found that the GC box within Zbtb7A promoter is necessary for the promoter activity. Furthermore, we identified that Sp1 acts as an activator in the regulation of Zbtb7A promoter activity and the physical interaction between Sp1 and GC box is responsible for the activation of Zbtb7A gene promoter.

Conclusion: Our results confirmed that Sp1 upregulates Zbtb7A gene expression via direct binding to GC box within the promoter.

No MeSH data available.


Related in: MedlinePlus