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Influence of very low doses of mediators on fungal laccase activity - nonlinearity beyond imagination.

Malarczyk E, Kochmanska-Rdest J, Jarosz-Wilkolazka A - Nonlinear Biomed Phys (2009)

Bottom Line: Two high laccase-producing fungal strains, Trametes versicolor and Cerrena unicolor, were used in this study as a source of enzyme.Selected dilutions of the mediators significantly increased the activity of extracellular laccase during 14 days of cultivation what was distinctly visible in PAGE technique and in colorimetric tests.The same mediator dilutions increased demethylation properties of laccase, which was demonstrated during incubation of enzyme with veratric acid.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biochemistry Department, Maria Curie-Skłodowska University, Lublin, Poland. malar@poczta.umcs.lublin.pl.

ABSTRACT
Laccase, an enzyme responsible for aerobic transformations of natural phenolics, in industrial applications requires the presence of low-molecular substances known as mediators, which accelerate oxidation processes. However, the use of mediators is limited by their toxicity and the high costs of exploitation. The activation of extracellular laccase in growing fungal culture with highly diluted mediators, ABTS and HBT is described. Two high laccase-producing fungal strains, Trametes versicolor and Cerrena unicolor, were used in this study as a source of enzyme. Selected dilutions of the mediators significantly increased the activity of extracellular laccase during 14 days of cultivation what was distinctly visible in PAGE technique and in colorimetric tests. The same mediator dilutions increased demethylation properties of laccase, which was demonstrated during incubation of enzyme with veratric acid. It was established that the activation effect was assigned to specific dilutions of mediators. Our dose-response dilution process smoothly passes into the range of action of homeopathic dilutions and is of interest for homeopaths.

No MeSH data available.


Related in: MedlinePlus

Demethylation of veratrate with media after 14 day cultivation of Trametes versicolor incubated 4 hours with extreme dilutions of ABTS chosen according to PAGE results on Figure 5; ctrl - control.
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Figure 6: Demethylation of veratrate with media after 14 day cultivation of Trametes versicolor incubated 4 hours with extreme dilutions of ABTS chosen according to PAGE results on Figure 5; ctrl - control.

Mentions: To characterize influence of different mediator dilutions on laccase activity the test with veratrate demethylation was done. In the first series of experiment with ABTS and T. versicolor, the abilities of chosen dilutions to initiate demethylation process were observed during the common incubation of the culture media, veratrate and proper mediator dilution (Figure 6). Because the generally low speedy of demethylation process [36] the time of four hours of incubation was chosen. The amount of demethylation products (mainly two isomeric vanillic acids) was determined spectrophotometrically with DASA reaction. The regular changes in demethylation activity were visible shaped like sine curve also for dilutions greater than the Avogadro number.


Influence of very low doses of mediators on fungal laccase activity - nonlinearity beyond imagination.

Malarczyk E, Kochmanska-Rdest J, Jarosz-Wilkolazka A - Nonlinear Biomed Phys (2009)

Demethylation of veratrate with media after 14 day cultivation of Trametes versicolor incubated 4 hours with extreme dilutions of ABTS chosen according to PAGE results on Figure 5; ctrl - control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2749849&req=5

Figure 6: Demethylation of veratrate with media after 14 day cultivation of Trametes versicolor incubated 4 hours with extreme dilutions of ABTS chosen according to PAGE results on Figure 5; ctrl - control.
Mentions: To characterize influence of different mediator dilutions on laccase activity the test with veratrate demethylation was done. In the first series of experiment with ABTS and T. versicolor, the abilities of chosen dilutions to initiate demethylation process were observed during the common incubation of the culture media, veratrate and proper mediator dilution (Figure 6). Because the generally low speedy of demethylation process [36] the time of four hours of incubation was chosen. The amount of demethylation products (mainly two isomeric vanillic acids) was determined spectrophotometrically with DASA reaction. The regular changes in demethylation activity were visible shaped like sine curve also for dilutions greater than the Avogadro number.

Bottom Line: Two high laccase-producing fungal strains, Trametes versicolor and Cerrena unicolor, were used in this study as a source of enzyme.Selected dilutions of the mediators significantly increased the activity of extracellular laccase during 14 days of cultivation what was distinctly visible in PAGE technique and in colorimetric tests.The same mediator dilutions increased demethylation properties of laccase, which was demonstrated during incubation of enzyme with veratric acid.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biochemistry Department, Maria Curie-Skłodowska University, Lublin, Poland. malar@poczta.umcs.lublin.pl.

ABSTRACT
Laccase, an enzyme responsible for aerobic transformations of natural phenolics, in industrial applications requires the presence of low-molecular substances known as mediators, which accelerate oxidation processes. However, the use of mediators is limited by their toxicity and the high costs of exploitation. The activation of extracellular laccase in growing fungal culture with highly diluted mediators, ABTS and HBT is described. Two high laccase-producing fungal strains, Trametes versicolor and Cerrena unicolor, were used in this study as a source of enzyme. Selected dilutions of the mediators significantly increased the activity of extracellular laccase during 14 days of cultivation what was distinctly visible in PAGE technique and in colorimetric tests. The same mediator dilutions increased demethylation properties of laccase, which was demonstrated during incubation of enzyme with veratric acid. It was established that the activation effect was assigned to specific dilutions of mediators. Our dose-response dilution process smoothly passes into the range of action of homeopathic dilutions and is of interest for homeopaths.

No MeSH data available.


Related in: MedlinePlus