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Poly(hydroxyalkanoates)-based polymeric nanoparticles for drug delivery.

Errico C, Bartoli C, Chiellini F, Chiellini E - J. Biomed. Biotechnol. (2009)

Bottom Line: The relevant results obtained give a theoretical understanding of the phenomenon occurring during colloidal formation.The adopted procedure allows for a relatively small diameter and homogeneity in size distribution of the PHB nanoparticles to be obtained compared to other methods like the one based on solvent evaporation which leads to particles on microscale.The biocompatibility of PHB and relative nanoparticles was investigated and both exhibited very good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Bioactive Polymeric Materials for Biomedical & Environmental Applications, UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Risorgimento 35, 56126 Pisa, Italy.

ABSTRACT
Poly (hydroxyalkanoates) (PHAs) have recently attracted a great deal of academic and industrial interest for their biodegradability and biocompatibility making them suitable for environmental and biomedical applications. Poly(3-hydroxybutyrate-) (PHB-) and Poly(DL-lactide-co-glycolide) (PLGA-) based nanoparticles were prepared using the dialysis method as yet unreported for the preparation of nanoparticles based on PHB. Processing conditions were varied in order to evaluate their influence on morphology, drug encapsulation, and size of nanoparticles. The relevant results obtained give a theoretical understanding of the phenomenon occurring during colloidal formation. The adopted procedure allows for a relatively small diameter and homogeneity in size distribution of the PHB nanoparticles to be obtained compared to other methods like the one based on solvent evaporation which leads to particles on microscale. The biocompatibility of PHB and relative nanoparticles was investigated and both exhibited very good cytocompatibility.

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SEM image and particle size distribution of LG-14 NPs.
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fig7: SEM image and particle size distribution of LG-14 NPs.

Mentions: Nanoparticles drug content resulted lower than expected. Attempts to increase the loading were carried out by dialysing the polymer, drug, and surfactant solution against ethanol instead of water during the first three hours of dialysis (LG-14). Ethanol acts as a nonsolvent for PLGA, allowing for the formation of nanoparticles and as a solvent for RA, avoiding the formation of flakes and precipitates. Nanoparticles with an average diameter size of 630 nm, with a very good spherical shape, and with a drug content of 2.2% were obtained (Figure 7).


Poly(hydroxyalkanoates)-based polymeric nanoparticles for drug delivery.

Errico C, Bartoli C, Chiellini F, Chiellini E - J. Biomed. Biotechnol. (2009)

SEM image and particle size distribution of LG-14 NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2749206&req=5

fig7: SEM image and particle size distribution of LG-14 NPs.
Mentions: Nanoparticles drug content resulted lower than expected. Attempts to increase the loading were carried out by dialysing the polymer, drug, and surfactant solution against ethanol instead of water during the first three hours of dialysis (LG-14). Ethanol acts as a nonsolvent for PLGA, allowing for the formation of nanoparticles and as a solvent for RA, avoiding the formation of flakes and precipitates. Nanoparticles with an average diameter size of 630 nm, with a very good spherical shape, and with a drug content of 2.2% were obtained (Figure 7).

Bottom Line: The relevant results obtained give a theoretical understanding of the phenomenon occurring during colloidal formation.The adopted procedure allows for a relatively small diameter and homogeneity in size distribution of the PHB nanoparticles to be obtained compared to other methods like the one based on solvent evaporation which leads to particles on microscale.The biocompatibility of PHB and relative nanoparticles was investigated and both exhibited very good cytocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Bioactive Polymeric Materials for Biomedical & Environmental Applications, UdR-INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, Via Risorgimento 35, 56126 Pisa, Italy.

ABSTRACT
Poly (hydroxyalkanoates) (PHAs) have recently attracted a great deal of academic and industrial interest for their biodegradability and biocompatibility making them suitable for environmental and biomedical applications. Poly(3-hydroxybutyrate-) (PHB-) and Poly(DL-lactide-co-glycolide) (PLGA-) based nanoparticles were prepared using the dialysis method as yet unreported for the preparation of nanoparticles based on PHB. Processing conditions were varied in order to evaluate their influence on morphology, drug encapsulation, and size of nanoparticles. The relevant results obtained give a theoretical understanding of the phenomenon occurring during colloidal formation. The adopted procedure allows for a relatively small diameter and homogeneity in size distribution of the PHB nanoparticles to be obtained compared to other methods like the one based on solvent evaporation which leads to particles on microscale. The biocompatibility of PHB and relative nanoparticles was investigated and both exhibited very good cytocompatibility.

Show MeSH