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Binding fullerenol C(60)(OH)(24) to dsDNA.

Pinteala M, Dascalu A, Ungurenasu C - Int J Nanomedicine (2009)

Bottom Line: The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported.The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1).Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

View Article: PubMed Central - PubMed

Affiliation: Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, 700487 Iasi, Romania.

ABSTRACT
The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1). Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

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Dependence of fluorescence intensity of C60(OH)24 on dsDNA concentration. A) Fluorescence spectra of fullerenol (– · –) with increasing concentration of DNA (—) with excitation at 420 nm ; B) Plot of fluorescence intensity of fullerenol as a function of [dsDNA] in the concentration range of 1.3 × 10−9 to 4.4 × 10−6 molL−1; [C60(OH)24] = 4.5 × 10−5 molL−1; average standard errors: 2.69% for 2.5E−5−5E−5 region and 10.80% for 1.3E−9−3.1E−6 region.
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f4-ijn-4-193: Dependence of fluorescence intensity of C60(OH)24 on dsDNA concentration. A) Fluorescence spectra of fullerenol (– · –) with increasing concentration of DNA (—) with excitation at 420 nm ; B) Plot of fluorescence intensity of fullerenol as a function of [dsDNA] in the concentration range of 1.3 × 10−9 to 4.4 × 10−6 molL−1; [C60(OH)24] = 4.5 × 10−5 molL−1; average standard errors: 2.69% for 2.5E−5−5E−5 region and 10.80% for 1.3E−9−3.1E−6 region.

Mentions: In order to evaluate the range of [DNA] determination, the binding of fullerenol to DNA was characterized through fluorescence emission titration of fullerenol. The enhancement of the fluorescence intensity of fullerenol with DNA at increasing concentrations is shown in Figure 4. One can observe that even for nanoscale concentration of DNA the fluorescence intensity of fullerenol increases from 25 to 100 (Figure 4a). The plot in Figure 4b is broken down into two regimes corresponding to ranges from 1.3 × 10−9 to 3.1 × 10−6 gL−1 and 2.5 × 10−5 to 5 × 10−5 gL−1. The low [DNA] range in the plots of Figure 4b (detection limit = 1.3 ng/mL) are close to what can be accomplished with current available fluorescence probes, ie, Hoechst 33258 (20 ng/mL) and YO-PRO-1/YOYO-1 (0.5–2.5 ng/mL). In addition, from the shape and intensity of emission spectrum recorded for [DNA] = 2.1 × 10−9 molL−1 (Figure 4a), it is useful to point out that the sensitivity can be extended into lower regions.


Binding fullerenol C(60)(OH)(24) to dsDNA.

Pinteala M, Dascalu A, Ungurenasu C - Int J Nanomedicine (2009)

Dependence of fluorescence intensity of C60(OH)24 on dsDNA concentration. A) Fluorescence spectra of fullerenol (– · –) with increasing concentration of DNA (—) with excitation at 420 nm ; B) Plot of fluorescence intensity of fullerenol as a function of [dsDNA] in the concentration range of 1.3 × 10−9 to 4.4 × 10−6 molL−1; [C60(OH)24] = 4.5 × 10−5 molL−1; average standard errors: 2.69% for 2.5E−5−5E−5 region and 10.80% for 1.3E−9−3.1E−6 region.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2748842&req=5

f4-ijn-4-193: Dependence of fluorescence intensity of C60(OH)24 on dsDNA concentration. A) Fluorescence spectra of fullerenol (– · –) with increasing concentration of DNA (—) with excitation at 420 nm ; B) Plot of fluorescence intensity of fullerenol as a function of [dsDNA] in the concentration range of 1.3 × 10−9 to 4.4 × 10−6 molL−1; [C60(OH)24] = 4.5 × 10−5 molL−1; average standard errors: 2.69% for 2.5E−5−5E−5 region and 10.80% for 1.3E−9−3.1E−6 region.
Mentions: In order to evaluate the range of [DNA] determination, the binding of fullerenol to DNA was characterized through fluorescence emission titration of fullerenol. The enhancement of the fluorescence intensity of fullerenol with DNA at increasing concentrations is shown in Figure 4. One can observe that even for nanoscale concentration of DNA the fluorescence intensity of fullerenol increases from 25 to 100 (Figure 4a). The plot in Figure 4b is broken down into two regimes corresponding to ranges from 1.3 × 10−9 to 3.1 × 10−6 gL−1 and 2.5 × 10−5 to 5 × 10−5 gL−1. The low [DNA] range in the plots of Figure 4b (detection limit = 1.3 ng/mL) are close to what can be accomplished with current available fluorescence probes, ie, Hoechst 33258 (20 ng/mL) and YO-PRO-1/YOYO-1 (0.5–2.5 ng/mL). In addition, from the shape and intensity of emission spectrum recorded for [DNA] = 2.1 × 10−9 molL−1 (Figure 4a), it is useful to point out that the sensitivity can be extended into lower regions.

Bottom Line: The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported.The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1).Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

View Article: PubMed Central - PubMed

Affiliation: Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, 700487 Iasi, Romania.

ABSTRACT
The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1). Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

Show MeSH
Related in: MedlinePlus