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Binding fullerenol C(60)(OH)(24) to dsDNA.

Pinteala M, Dascalu A, Ungurenasu C - Int J Nanomedicine (2009)

Bottom Line: The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported.The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1).Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

View Article: PubMed Central - PubMed

Affiliation: Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, 700487 Iasi, Romania.

ABSTRACT
The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1). Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

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Fluorescence emission spectra of fullerenol ( - - - ) and dsDNA (——) for different excitation wavelength. A) λ = 420 nm. B) λ = 380 nm. C) λ = 360 nm. D) λ = 340 nm. [C60(OH)24] = 1 × 10−4 molL−1; [DNA] = 1 × 10−4 molL−1.
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f2-ijn-4-193: Fluorescence emission spectra of fullerenol ( - - - ) and dsDNA (——) for different excitation wavelength. A) λ = 420 nm. B) λ = 380 nm. C) λ = 360 nm. D) λ = 340 nm. [C60(OH)24] = 1 × 10−4 molL−1; [DNA] = 1 × 10−4 molL−1.

Mentions: Figure 2 shows the fullerenol and DNA emission spectra recorded at different excitation wavelengths. Inspection of how fluorescence emission spectra of fullerenol and DNA change as a function of excitation wavelength yield additional supporting information on the appropriate fullerenol excitation wavelength suitable for the fluorescence investigation of C60(OH)24 – DNA complex in aqueous media. One can observe that for a concentration higher than 1.6 × 10−5 molL−1, the emission spectra of fullerenol do not overlap with emission spectra of DNA when excited at 340, 360, 380, and 420 nm, respectively. Apparently, all these fluorescence excitations should be suitable for a fluorescence study of DNA-fullerenol interaction. However, the emission maxima of fullerenol at concentrations <1.6 × 10−5 molL−1 (Figure 1a) and DNA (Figure 2a) overlap at 492 nm when recorded at λex = 420 nm. This is the reason why, to cover a large concentration range (1−9−4.5−5 molL−1) of fullerenol, the fluorescence excitation at 420 nm and emission at 469 nm were used for fluorescence intensity measurements in this work.


Binding fullerenol C(60)(OH)(24) to dsDNA.

Pinteala M, Dascalu A, Ungurenasu C - Int J Nanomedicine (2009)

Fluorescence emission spectra of fullerenol ( - - - ) and dsDNA (——) for different excitation wavelength. A) λ = 420 nm. B) λ = 380 nm. C) λ = 360 nm. D) λ = 340 nm. [C60(OH)24] = 1 × 10−4 molL−1; [DNA] = 1 × 10−4 molL−1.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2748842&req=5

f2-ijn-4-193: Fluorescence emission spectra of fullerenol ( - - - ) and dsDNA (——) for different excitation wavelength. A) λ = 420 nm. B) λ = 380 nm. C) λ = 360 nm. D) λ = 340 nm. [C60(OH)24] = 1 × 10−4 molL−1; [DNA] = 1 × 10−4 molL−1.
Mentions: Figure 2 shows the fullerenol and DNA emission spectra recorded at different excitation wavelengths. Inspection of how fluorescence emission spectra of fullerenol and DNA change as a function of excitation wavelength yield additional supporting information on the appropriate fullerenol excitation wavelength suitable for the fluorescence investigation of C60(OH)24 – DNA complex in aqueous media. One can observe that for a concentration higher than 1.6 × 10−5 molL−1, the emission spectra of fullerenol do not overlap with emission spectra of DNA when excited at 340, 360, 380, and 420 nm, respectively. Apparently, all these fluorescence excitations should be suitable for a fluorescence study of DNA-fullerenol interaction. However, the emission maxima of fullerenol at concentrations <1.6 × 10−5 molL−1 (Figure 1a) and DNA (Figure 2a) overlap at 492 nm when recorded at λex = 420 nm. This is the reason why, to cover a large concentration range (1−9−4.5−5 molL−1) of fullerenol, the fluorescence excitation at 420 nm and emission at 469 nm were used for fluorescence intensity measurements in this work.

Bottom Line: The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported.The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1).Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

View Article: PubMed Central - PubMed

Affiliation: Petru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica, 700487 Iasi, Romania.

ABSTRACT
The first C(60)(OH)(24)-DNA complex and its fluorescence enhancement is reported. The enhanced fluorescence intensity of fullerenol C(60)(OH)(24) is in proportion to the concentration of DNA in the range of 1 x 10(-9) to 8 x 10(-5) molL(-1) and the detection limit was 1.3 ng mL(-1). Fullerenol C(60)(OH)(24) binds significantly to the phosphate backbone of native dsDNA and to base-pairs within the major groove of sodium salt of dsDNA.

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