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Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinoma.

Zhou ZQ, Cao WH, Xie JJ, Lin J, Shen ZY, Zhang QY, Shen JH, Xu LY, Li EM - BMC Cancer (2009)

Bottom Line: Thrombospondin1 (THBS1), cystene-rich protein 61 (Cyr61) and connective tissue growth factor (CTGF) are all involved in the transforming growth factor-beta (TGF-beta) signal pathway, which plays an important role in the tumorigenesis.Correlations of THBS1, Cyr61 and CTGF over-expressions with various clinicopathologic factors were also determined by using the Chi-square test or Fisher's exact probability test.THBS1 over-expression was significantly associated with TNM stage (P = 0.029) and regional lymph node involvement (P = 0.026).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Shantou University, Shantou, PR China. s_zqzhou@163.com

ABSTRACT

Background: Thrombospondin1 (THBS1), cystene-rich protein 61 (Cyr61) and connective tissue growth factor (CTGF) are all involved in the transforming growth factor-beta (TGF-beta) signal pathway, which plays an important role in the tumorigenesis. The purpose of this study is to explore the expression and prognostic significance of these proteins in esophageal squamous cell carcinoma (ESCC).

Methods: We used immunohistochemistry and western blotting to examine the expression status of THBS1, Cyr61 and CTGF in ESCC. Correlations of THBS1, Cyr61 and CTGF over-expressions with various clinicopathologic factors were also determined by using the Chi-square test or Fisher's exact probability test. Survival analysis was assessed by the Kaplan-Meier analysis and the log-rank test. Relative risk was evaluated by the multivariate Cox proportional hazards model.

Results: THBS1, Cyr61 and CTGF were all over-expressed in ESCC. THBS1 over-expression was significantly associated with TNM stage (P = 0.029) and regional lymph node involvement (P = 0.026). Kaplan-Meier survival analysis showed that over-expression of THBS1, Cyr61 or CTGF was related to poor survival of ESCC patients (P = 0.042, P = 0.020, P = 0.018, respectively). Multivariate Cox analysis demonstrated that Cyr61 and CTGF were independent factors in prognosis of ESCC.

Conclusion: Cyr61, CTGF and THBS1 were all over-expressed in ESCC and might be new molecular markers to predict the prognosis of ESCC patients.

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Related in: MedlinePlus

Expressions of CTGF and CYR61 protein in four, randomly picked, paired ESCC samples were analyzed by western blotting. Signal intensities for the expression of p-ERK1/2 or p-Smad2/3 were quantified by densitometric scanning and normalized by internal control (β-actin). CTGF and CYR61 were expressed predominantly in ESCC tissues compared with the match normal tissues.
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Figure 2: Expressions of CTGF and CYR61 protein in four, randomly picked, paired ESCC samples were analyzed by western blotting. Signal intensities for the expression of p-ERK1/2 or p-Smad2/3 were quantified by densitometric scanning and normalized by internal control (β-actin). CTGF and CYR61 were expressed predominantly in ESCC tissues compared with the match normal tissues.

Mentions: In ESCC, THBS1, Cyr61 and CTGF immunoreactivities all displayed intense diffuse cytoplasmic staining (Figure 1A, C and 1E). In contrast, these proteins just showed weak staining in the basal and suprabasal proliferative layers of normal epithelium (Figure 1B, D and 1F). Over-expression was defined as intense diffuse cytoplasmic staining in >25% of tumor cells. Hence, 72/80, 41/80 and 39/80 for THBS1, Cyr61 and CTGF were respectively designated as over-expressions. In comparison with the staining in normal epithelial tissue, their over-expressions were all statistically significant (P < 0.05). In addition, elevated levels of CTGF and CYR61 protein were also found in human ESCC tissues compared with the paired normal tissue from the patients as shown by western blotting analysis (Figure 2).


Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinoma.

Zhou ZQ, Cao WH, Xie JJ, Lin J, Shen ZY, Zhang QY, Shen JH, Xu LY, Li EM - BMC Cancer (2009)

Expressions of CTGF and CYR61 protein in four, randomly picked, paired ESCC samples were analyzed by western blotting. Signal intensities for the expression of p-ERK1/2 or p-Smad2/3 were quantified by densitometric scanning and normalized by internal control (β-actin). CTGF and CYR61 were expressed predominantly in ESCC tissues compared with the match normal tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2748090&req=5

Figure 2: Expressions of CTGF and CYR61 protein in four, randomly picked, paired ESCC samples were analyzed by western blotting. Signal intensities for the expression of p-ERK1/2 or p-Smad2/3 were quantified by densitometric scanning and normalized by internal control (β-actin). CTGF and CYR61 were expressed predominantly in ESCC tissues compared with the match normal tissues.
Mentions: In ESCC, THBS1, Cyr61 and CTGF immunoreactivities all displayed intense diffuse cytoplasmic staining (Figure 1A, C and 1E). In contrast, these proteins just showed weak staining in the basal and suprabasal proliferative layers of normal epithelium (Figure 1B, D and 1F). Over-expression was defined as intense diffuse cytoplasmic staining in >25% of tumor cells. Hence, 72/80, 41/80 and 39/80 for THBS1, Cyr61 and CTGF were respectively designated as over-expressions. In comparison with the staining in normal epithelial tissue, their over-expressions were all statistically significant (P < 0.05). In addition, elevated levels of CTGF and CYR61 protein were also found in human ESCC tissues compared with the paired normal tissue from the patients as shown by western blotting analysis (Figure 2).

Bottom Line: Thrombospondin1 (THBS1), cystene-rich protein 61 (Cyr61) and connective tissue growth factor (CTGF) are all involved in the transforming growth factor-beta (TGF-beta) signal pathway, which plays an important role in the tumorigenesis.Correlations of THBS1, Cyr61 and CTGF over-expressions with various clinicopathologic factors were also determined by using the Chi-square test or Fisher's exact probability test.THBS1 over-expression was significantly associated with TNM stage (P = 0.029) and regional lymph node involvement (P = 0.026).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Shantou University, Shantou, PR China. s_zqzhou@163.com

ABSTRACT

Background: Thrombospondin1 (THBS1), cystene-rich protein 61 (Cyr61) and connective tissue growth factor (CTGF) are all involved in the transforming growth factor-beta (TGF-beta) signal pathway, which plays an important role in the tumorigenesis. The purpose of this study is to explore the expression and prognostic significance of these proteins in esophageal squamous cell carcinoma (ESCC).

Methods: We used immunohistochemistry and western blotting to examine the expression status of THBS1, Cyr61 and CTGF in ESCC. Correlations of THBS1, Cyr61 and CTGF over-expressions with various clinicopathologic factors were also determined by using the Chi-square test or Fisher's exact probability test. Survival analysis was assessed by the Kaplan-Meier analysis and the log-rank test. Relative risk was evaluated by the multivariate Cox proportional hazards model.

Results: THBS1, Cyr61 and CTGF were all over-expressed in ESCC. THBS1 over-expression was significantly associated with TNM stage (P = 0.029) and regional lymph node involvement (P = 0.026). Kaplan-Meier survival analysis showed that over-expression of THBS1, Cyr61 or CTGF was related to poor survival of ESCC patients (P = 0.042, P = 0.020, P = 0.018, respectively). Multivariate Cox analysis demonstrated that Cyr61 and CTGF were independent factors in prognosis of ESCC.

Conclusion: Cyr61, CTGF and THBS1 were all over-expressed in ESCC and might be new molecular markers to predict the prognosis of ESCC patients.

Show MeSH
Related in: MedlinePlus