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Comparison of exhaled breath condensate pH using two commercially available devices in healthy controls, asthma and COPD patients.

Koczulla R, Dragonieri S, Schot R, Bals R, Gauw SA, Vogelmeier C, Rabe KF, Sterk PJ, Hiemstra PS - Respir. Res. (2009)

Bottom Line: Analysis of exhaled breath condensate (EBC) is a non-invasive method for studying the acidity (pH) of airway secretions in patients with inflammatory lung diseases.EBC was collected from 40 subjects (n = 10 in each of the above groups) using RTube and ECoScreen.EBC was collected from controls on two separate occasions within 5 days. pH in EBC was assessed after degasification with argon for 20 min.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pulmonology, Leiden University Medical Center, the Netherlands. rembertkoczulla@web.de

ABSTRACT

Background: Analysis of exhaled breath condensate (EBC) is a non-invasive method for studying the acidity (pH) of airway secretions in patients with inflammatory lung diseases.

Aim: To assess the reproducibility of EBC pH for two commercially available devices (portable RTube and non-portable ECoScreen) in healthy controls, patients with asthma or COPD, and subjects suffering from an acute cold with lower-airway symptoms. In addition, we assessed the repeatability in healthy controls.

Methods: EBC was collected from 40 subjects (n = 10 in each of the above groups) using RTube and ECoScreen. EBC was collected from controls on two separate occasions within 5 days. pH in EBC was assessed after degasification with argon for 20 min.

Results: In controls, pH-measurements in EBC collected by RTube or ECoScreen showed no significant difference between devices (p = 0.754) or between days (repeatability coefficient RTube: 0.47; ECoScreen: 0.42) of collection. A comparison between EBC pH collected by the two devices in asthma, COPD and cold patients also showed good reproducibility. No differences in pH values were observed between controls (mean pH 8.27; RTube) and patients with COPD (pH 7.97) or asthma (pH 8.20), but lower values were found using both devices in patients with a cold (pH 7.56; RTube, p < 0.01; ECoScreen, p < 0.05).

Conclusion: We conclude that pH measurements in EBC collected by RTube and ECoScreen are repeatable and reproducible in healthy controls, and are reproducible and comparable in healthy controls, COPD and asthma patients, and subjects with a common cold.

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Related in: MedlinePlus

Bland-Altman plot showing within-subject repeatability of pH in EBC collected by ECoScreen (Figure 2A) and RTube (Figure 2B) from 10 healthy subjects on two different days. Horizontal lines show the mean and 95% confidence interval.
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Figure 2: Bland-Altman plot showing within-subject repeatability of pH in EBC collected by ECoScreen (Figure 2A) and RTube (Figure 2B) from 10 healthy subjects on two different days. Horizontal lines show the mean and 95% confidence interval.

Mentions: Between-day repeatability of the two methods was assessed in order to compare the performance of both devices.


Comparison of exhaled breath condensate pH using two commercially available devices in healthy controls, asthma and COPD patients.

Koczulla R, Dragonieri S, Schot R, Bals R, Gauw SA, Vogelmeier C, Rabe KF, Sterk PJ, Hiemstra PS - Respir. Res. (2009)

Bland-Altman plot showing within-subject repeatability of pH in EBC collected by ECoScreen (Figure 2A) and RTube (Figure 2B) from 10 healthy subjects on two different days. Horizontal lines show the mean and 95% confidence interval.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2747836&req=5

Figure 2: Bland-Altman plot showing within-subject repeatability of pH in EBC collected by ECoScreen (Figure 2A) and RTube (Figure 2B) from 10 healthy subjects on two different days. Horizontal lines show the mean and 95% confidence interval.
Mentions: Between-day repeatability of the two methods was assessed in order to compare the performance of both devices.

Bottom Line: Analysis of exhaled breath condensate (EBC) is a non-invasive method for studying the acidity (pH) of airway secretions in patients with inflammatory lung diseases.EBC was collected from 40 subjects (n = 10 in each of the above groups) using RTube and ECoScreen.EBC was collected from controls on two separate occasions within 5 days. pH in EBC was assessed after degasification with argon for 20 min.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pulmonology, Leiden University Medical Center, the Netherlands. rembertkoczulla@web.de

ABSTRACT

Background: Analysis of exhaled breath condensate (EBC) is a non-invasive method for studying the acidity (pH) of airway secretions in patients with inflammatory lung diseases.

Aim: To assess the reproducibility of EBC pH for two commercially available devices (portable RTube and non-portable ECoScreen) in healthy controls, patients with asthma or COPD, and subjects suffering from an acute cold with lower-airway symptoms. In addition, we assessed the repeatability in healthy controls.

Methods: EBC was collected from 40 subjects (n = 10 in each of the above groups) using RTube and ECoScreen. EBC was collected from controls on two separate occasions within 5 days. pH in EBC was assessed after degasification with argon for 20 min.

Results: In controls, pH-measurements in EBC collected by RTube or ECoScreen showed no significant difference between devices (p = 0.754) or between days (repeatability coefficient RTube: 0.47; ECoScreen: 0.42) of collection. A comparison between EBC pH collected by the two devices in asthma, COPD and cold patients also showed good reproducibility. No differences in pH values were observed between controls (mean pH 8.27; RTube) and patients with COPD (pH 7.97) or asthma (pH 8.20), but lower values were found using both devices in patients with a cold (pH 7.56; RTube, p < 0.01; ECoScreen, p < 0.05).

Conclusion: We conclude that pH measurements in EBC collected by RTube and ECoScreen are repeatable and reproducible in healthy controls, and are reproducible and comparable in healthy controls, COPD and asthma patients, and subjects with a common cold.

Show MeSH
Related in: MedlinePlus