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Allelic losses on chromosome 3p are accumulated in relation to morphological changes of lung adenocarcinoma.

Iijima H, Tomizawa Y, Dobashi K, Saito R, Nakajima T, Mori M - Br. J. Cancer (2004)

Bottom Line: Identical allelic patterns among various grades of differentiation were found in eight cases.Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases.Various allelic patterns among various grades of differentiation were found in three cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511, Japan.

ABSTRACT
We performed allelotyping analysis at nine regions on chromosome 3p using 56 microdissected samples from 23 primary lung adenocarcinomas to examine the process of progression within individual lung adenocarcinoma with various grades of differentiation. Identical allelic patterns among various grades of differentiation were found in eight cases. Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases. Various allelic patterns among various grades of differentiation were found in three cases. These results suggested that allelic losses on 3p play an important role in morphological changes of lung adenocarcinomas. We also investigated the relationship between allelic losses on 3p and histological subtypes of lung adenocarcinoma. The frequencies of allelic losses at 3p14.2 and telomeric region of 3p21.3 were higher in papillary type tumour (nine out of 14, 64% and 11 out of 15, 73%) than in bronchioloalveolar carcinoma-type tumour (one out of 8, 13%; P=0.031 and four out of 12, 33%; P = 0.057). These results indicated that allelic losses at 3p14.2 and telomeric region of 3p21.3 are related to pattern of the proliferation of lung adenocarcinoma.

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Representative autoradiographs of microsatellite analyses for LOH using multiple 3p markers in the microdissected primary lung adenocarcinoma and normal epithelium from a cancer patient (No. 477). The markers are indicated on the left of the autoradiograph panel. Black arrowheads indicate main allele bands and white arrowheads indicate microsatellite instability bands. Asterisks indicate allelic loss. Microsatellite instabilities were seen at D3S1573 and D3S1274. W=well-differentiated portion; M=moderately differentiated portion; P=poorly differentiated portion; N=histologically normal epithelium.
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fig2: Representative autoradiographs of microsatellite analyses for LOH using multiple 3p markers in the microdissected primary lung adenocarcinoma and normal epithelium from a cancer patient (No. 477). The markers are indicated on the left of the autoradiograph panel. Black arrowheads indicate main allele bands and white arrowheads indicate microsatellite instability bands. Asterisks indicate allelic loss. Microsatellite instabilities were seen at D3S1573 and D3S1274. W=well-differentiated portion; M=moderately differentiated portion; P=poorly differentiated portion; N=histologically normal epithelium.

Mentions: LOH of chromosome 3p was examined in 56 portions with various grades of differentiation from 23 lung adenocarcinomas by PCR amplification using nine microsatellite polymorphic markers distributed across chromosome 3p. Representative results of LOH analysis are shown in Figure 2Figure 2


Allelic losses on chromosome 3p are accumulated in relation to morphological changes of lung adenocarcinoma.

Iijima H, Tomizawa Y, Dobashi K, Saito R, Nakajima T, Mori M - Br. J. Cancer (2004)

Representative autoradiographs of microsatellite analyses for LOH using multiple 3p markers in the microdissected primary lung adenocarcinoma and normal epithelium from a cancer patient (No. 477). The markers are indicated on the left of the autoradiograph panel. Black arrowheads indicate main allele bands and white arrowheads indicate microsatellite instability bands. Asterisks indicate allelic loss. Microsatellite instabilities were seen at D3S1573 and D3S1274. W=well-differentiated portion; M=moderately differentiated portion; P=poorly differentiated portion; N=histologically normal epithelium.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747704&req=5

fig2: Representative autoradiographs of microsatellite analyses for LOH using multiple 3p markers in the microdissected primary lung adenocarcinoma and normal epithelium from a cancer patient (No. 477). The markers are indicated on the left of the autoradiograph panel. Black arrowheads indicate main allele bands and white arrowheads indicate microsatellite instability bands. Asterisks indicate allelic loss. Microsatellite instabilities were seen at D3S1573 and D3S1274. W=well-differentiated portion; M=moderately differentiated portion; P=poorly differentiated portion; N=histologically normal epithelium.
Mentions: LOH of chromosome 3p was examined in 56 portions with various grades of differentiation from 23 lung adenocarcinomas by PCR amplification using nine microsatellite polymorphic markers distributed across chromosome 3p. Representative results of LOH analysis are shown in Figure 2Figure 2

Bottom Line: Identical allelic patterns among various grades of differentiation were found in eight cases.Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases.Various allelic patterns among various grades of differentiation were found in three cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511, Japan.

ABSTRACT
We performed allelotyping analysis at nine regions on chromosome 3p using 56 microdissected samples from 23 primary lung adenocarcinomas to examine the process of progression within individual lung adenocarcinoma with various grades of differentiation. Identical allelic patterns among various grades of differentiation were found in eight cases. Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases. Various allelic patterns among various grades of differentiation were found in three cases. These results suggested that allelic losses on 3p play an important role in morphological changes of lung adenocarcinomas. We also investigated the relationship between allelic losses on 3p and histological subtypes of lung adenocarcinoma. The frequencies of allelic losses at 3p14.2 and telomeric region of 3p21.3 were higher in papillary type tumour (nine out of 14, 64% and 11 out of 15, 73%) than in bronchioloalveolar carcinoma-type tumour (one out of 8, 13%; P=0.031 and four out of 12, 33%; P = 0.057). These results indicated that allelic losses at 3p14.2 and telomeric region of 3p21.3 are related to pattern of the proliferation of lung adenocarcinoma.

Show MeSH
Related in: MedlinePlus