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Overexpression of leucocyte common antigen (LAR) P-subunit in thyroid carcinomas.

Konishi N, Tsujikawa K, Yamamoto H, Ishida E, Nakamura M, Shimada K, Yane K, Yamashita H, Noguchi S - Br. J. Cancer (2003)

Bottom Line: In contrast to broad staining in carcinomas, LAR expression in thyroid adenomas was often found in small focal or locally invasive areas.Western blot analysis similarly detected LAR P-subunit protein in thyroid carcinomas, but not in normal tissues.We believe this to be the first demonstration of LAR overexpression in thyroid carcinoma and may help to elucidate the role of PTPases in the development of malignancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Nara Medical University, Nara, Japan. nkonishi@naramed-u.ac.jp

ABSTRACT
Protein tyrosine phosphatase (PTPase) dephosphorylation and protein tyrosine kinase (PTKs) phosphorylation of key signal transduction proteins may be regulated by extracellular signals, making PTPases important in the regulation of cell proliferation. Leucocyte common antigen (LAR), a receptor-like PTPase, consists of E-subunit, containing the cell adhesion molecule-like receptor region, and P-subunit specific for a short segment of the extracellular region, the transmembrane peptide, and two cytoplasmic PTPase domains. We produced a monoclonal antibody against the LAR P-subunit for immunohistochemical screening of LAR expression in normal and tumourous tissues. Gliomas and gastric, colorectal, lung, breast and prostate cancers showed weak and relatively infrequent expression. Intense and diffuse expression, however, was detected in 95% (227 out of 239) of thyroid carcinomas, but only 12% (22 out of 128) of adenomas and no cases of benign thyroid disease were immunopositive. In contrast to broad staining in carcinomas, LAR expression in thyroid adenomas was often found in small focal or locally invasive areas. Western blot analysis similarly detected LAR P-subunit protein in thyroid carcinomas, but not in normal tissues. We believe this to be the first demonstration of LAR overexpression in thyroid carcinoma and may help to elucidate the role of PTPases in the development of malignancy.

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Immunohistochemical expression of the LAR P-subunit (YU1) in thyroid tumours. (A) papillary carcinoma, judged as (++), while the normal thyroid glands were negative (× 100), (B) papillary carcinoma, metastatic to lymph node (× 100), (C) follicular adenoma showing focally positive immunostaining, judged as (+) because positive tumour cells were 20–50% in the lesion (× 100), (D) follicular adenoma showing strong positive staining in invasive areas (diagnosed as invasive adenoma) (× 40).
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fig3: Immunohistochemical expression of the LAR P-subunit (YU1) in thyroid tumours. (A) papillary carcinoma, judged as (++), while the normal thyroid glands were negative (× 100), (B) papillary carcinoma, metastatic to lymph node (× 100), (C) follicular adenoma showing focally positive immunostaining, judged as (+) because positive tumour cells were 20–50% in the lesion (× 100), (D) follicular adenoma showing strong positive staining in invasive areas (diagnosed as invasive adenoma) (× 40).

Mentions: Immunoreactivity was expressed as: (−) <20% of cells immunopositive; (+) 20–50% of cells immunopositive; (++) >50% of cells immunopositive.


Overexpression of leucocyte common antigen (LAR) P-subunit in thyroid carcinomas.

Konishi N, Tsujikawa K, Yamamoto H, Ishida E, Nakamura M, Shimada K, Yane K, Yamashita H, Noguchi S - Br. J. Cancer (2003)

Immunohistochemical expression of the LAR P-subunit (YU1) in thyroid tumours. (A) papillary carcinoma, judged as (++), while the normal thyroid glands were negative (× 100), (B) papillary carcinoma, metastatic to lymph node (× 100), (C) follicular adenoma showing focally positive immunostaining, judged as (+) because positive tumour cells were 20–50% in the lesion (× 100), (D) follicular adenoma showing strong positive staining in invasive areas (diagnosed as invasive adenoma) (× 40).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747580&req=5

fig3: Immunohistochemical expression of the LAR P-subunit (YU1) in thyroid tumours. (A) papillary carcinoma, judged as (++), while the normal thyroid glands were negative (× 100), (B) papillary carcinoma, metastatic to lymph node (× 100), (C) follicular adenoma showing focally positive immunostaining, judged as (+) because positive tumour cells were 20–50% in the lesion (× 100), (D) follicular adenoma showing strong positive staining in invasive areas (diagnosed as invasive adenoma) (× 40).
Mentions: Immunoreactivity was expressed as: (−) <20% of cells immunopositive; (+) 20–50% of cells immunopositive; (++) >50% of cells immunopositive.

Bottom Line: In contrast to broad staining in carcinomas, LAR expression in thyroid adenomas was often found in small focal or locally invasive areas.Western blot analysis similarly detected LAR P-subunit protein in thyroid carcinomas, but not in normal tissues.We believe this to be the first demonstration of LAR overexpression in thyroid carcinoma and may help to elucidate the role of PTPases in the development of malignancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Nara Medical University, Nara, Japan. nkonishi@naramed-u.ac.jp

ABSTRACT
Protein tyrosine phosphatase (PTPase) dephosphorylation and protein tyrosine kinase (PTKs) phosphorylation of key signal transduction proteins may be regulated by extracellular signals, making PTPases important in the regulation of cell proliferation. Leucocyte common antigen (LAR), a receptor-like PTPase, consists of E-subunit, containing the cell adhesion molecule-like receptor region, and P-subunit specific for a short segment of the extracellular region, the transmembrane peptide, and two cytoplasmic PTPase domains. We produced a monoclonal antibody against the LAR P-subunit for immunohistochemical screening of LAR expression in normal and tumourous tissues. Gliomas and gastric, colorectal, lung, breast and prostate cancers showed weak and relatively infrequent expression. Intense and diffuse expression, however, was detected in 95% (227 out of 239) of thyroid carcinomas, but only 12% (22 out of 128) of adenomas and no cases of benign thyroid disease were immunopositive. In contrast to broad staining in carcinomas, LAR expression in thyroid adenomas was often found in small focal or locally invasive areas. Western blot analysis similarly detected LAR P-subunit protein in thyroid carcinomas, but not in normal tissues. We believe this to be the first demonstration of LAR overexpression in thyroid carcinoma and may help to elucidate the role of PTPases in the development of malignancy.

Show MeSH
Related in: MedlinePlus