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Cooperation between monocytes and breast cancer cells promotes factors involved in cancer aggressiveness.

Blot E, Chen W, Vasse M, Paysant J, Denoyelle C, Pillé JY, Vincent L, Vannier JP, Soria J, Soria C - Br. J. Cancer (2003)

Bottom Line: In breast cancers, clinical symptoms of inflammation localised around the tumour at the time of diagnosis have been considered to have poor prognosis significance.The incubation of the breast-cancer-derived MDA-MB231 cells with monocytes resulted in an increase in factors involved in cell invasion (i.e. both cancer cells and monocytes-associated urokinase and Tissue Factor, and PAI-1 and MMP-9 secretion).Incubation of monocytes with MDA-MB231 cancer cells resulted in a downregulation in the secretion of the antiproliferative cytokine Oncostatin M, while the apoptotic factor TNF alpha was dramatically increased.

View Article: PubMed Central - PubMed

Affiliation: DIFEMA Laboratory, Medicine and Pharmacy Faculty, Cedex, France. eblot@rouen.fnclcc.fr

ABSTRACT
In breast cancers, clinical symptoms of inflammation localised around the tumour at the time of diagnosis have been considered to have poor prognosis significance. In this study, the biological mechanisms responsible for the deleterious action of monocytes in cancer were investigated. The incubation of the breast-cancer-derived MDA-MB231 cells with monocytes resulted in an increase in factors involved in cell invasion (i.e. both cancer cells and monocytes-associated urokinase and Tissue Factor, and PAI-1 and MMP-9 secretion). Moreover, the functions of monocytes were also modified. Incubation of monocytes with MDA-MB231 cancer cells resulted in a downregulation in the secretion of the antiproliferative cytokine Oncostatin M, while the apoptotic factor TNF alpha was dramatically increased. However, MDA-MB231 cancer cells have been shown to be resistant towards the apoptotic action of TNF alpha. These findings demonstrate that incubation of MDA-MB231 cancer cells with monocytes induced a crosstalk, which resulted in an increased expression of factors involved in cancer cell invasiveness and in a modification of monocytes function against cancer cells, while inflammatory effects were increased.

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Effect of incubation of MDA-MB231 with monocytes on u-PA and u-PAR expression on each cell type. The u-PA and u-PAR expression was calculated as the percentage of fluorescence as compared with MDA-MB231 and monocytes alone (n=4): (A) measurement of u-PA associated to MDA-MB231 cancer cells, (B) measurement of u-PA associated to monocytes, (C) measurement of u-PAR associated to MDA-MB231 cancer cells, (D) measurement of u-PAR associated to monocytes.
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fig2: Effect of incubation of MDA-MB231 with monocytes on u-PA and u-PAR expression on each cell type. The u-PA and u-PAR expression was calculated as the percentage of fluorescence as compared with MDA-MB231 and monocytes alone (n=4): (A) measurement of u-PA associated to MDA-MB231 cancer cells, (B) measurement of u-PA associated to monocytes, (C) measurement of u-PAR associated to MDA-MB231 cancer cells, (D) measurement of u-PAR associated to monocytes.

Mentions: Membrane expression of u-PA was spontaneously high on MDA-MB231 cancer cells incubated alone and was low on monocytes incubated alone. After incubation of MDA-MB231 cancer cells with monocytes, the u-PA associated with the cell membrane increased in both monocytes and MDA-MB231 cancer cells, as shown in Figure 2Figure 2


Cooperation between monocytes and breast cancer cells promotes factors involved in cancer aggressiveness.

Blot E, Chen W, Vasse M, Paysant J, Denoyelle C, Pillé JY, Vincent L, Vannier JP, Soria J, Soria C - Br. J. Cancer (2003)

Effect of incubation of MDA-MB231 with monocytes on u-PA and u-PAR expression on each cell type. The u-PA and u-PAR expression was calculated as the percentage of fluorescence as compared with MDA-MB231 and monocytes alone (n=4): (A) measurement of u-PA associated to MDA-MB231 cancer cells, (B) measurement of u-PA associated to monocytes, (C) measurement of u-PAR associated to MDA-MB231 cancer cells, (D) measurement of u-PAR associated to monocytes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747575&req=5

fig2: Effect of incubation of MDA-MB231 with monocytes on u-PA and u-PAR expression on each cell type. The u-PA and u-PAR expression was calculated as the percentage of fluorescence as compared with MDA-MB231 and monocytes alone (n=4): (A) measurement of u-PA associated to MDA-MB231 cancer cells, (B) measurement of u-PA associated to monocytes, (C) measurement of u-PAR associated to MDA-MB231 cancer cells, (D) measurement of u-PAR associated to monocytes.
Mentions: Membrane expression of u-PA was spontaneously high on MDA-MB231 cancer cells incubated alone and was low on monocytes incubated alone. After incubation of MDA-MB231 cancer cells with monocytes, the u-PA associated with the cell membrane increased in both monocytes and MDA-MB231 cancer cells, as shown in Figure 2Figure 2

Bottom Line: In breast cancers, clinical symptoms of inflammation localised around the tumour at the time of diagnosis have been considered to have poor prognosis significance.The incubation of the breast-cancer-derived MDA-MB231 cells with monocytes resulted in an increase in factors involved in cell invasion (i.e. both cancer cells and monocytes-associated urokinase and Tissue Factor, and PAI-1 and MMP-9 secretion).Incubation of monocytes with MDA-MB231 cancer cells resulted in a downregulation in the secretion of the antiproliferative cytokine Oncostatin M, while the apoptotic factor TNF alpha was dramatically increased.

View Article: PubMed Central - PubMed

Affiliation: DIFEMA Laboratory, Medicine and Pharmacy Faculty, Cedex, France. eblot@rouen.fnclcc.fr

ABSTRACT
In breast cancers, clinical symptoms of inflammation localised around the tumour at the time of diagnosis have been considered to have poor prognosis significance. In this study, the biological mechanisms responsible for the deleterious action of monocytes in cancer were investigated. The incubation of the breast-cancer-derived MDA-MB231 cells with monocytes resulted in an increase in factors involved in cell invasion (i.e. both cancer cells and monocytes-associated urokinase and Tissue Factor, and PAI-1 and MMP-9 secretion). Moreover, the functions of monocytes were also modified. Incubation of monocytes with MDA-MB231 cancer cells resulted in a downregulation in the secretion of the antiproliferative cytokine Oncostatin M, while the apoptotic factor TNF alpha was dramatically increased. However, MDA-MB231 cancer cells have been shown to be resistant towards the apoptotic action of TNF alpha. These findings demonstrate that incubation of MDA-MB231 cancer cells with monocytes induced a crosstalk, which resulted in an increased expression of factors involved in cancer cell invasiveness and in a modification of monocytes function against cancer cells, while inflammatory effects were increased.

Show MeSH
Related in: MedlinePlus