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A role for BRCA1 in sporadic breast cancer.

Fraser JA, Reeves JR, Stanton PD, Black DM, Going JJ, Cooke TG, Bartlett JM - Br. J. Cancer (2003)

Bottom Line: Intense labelling predicted decreased overall survival (P=0.012), disease-free survival (P=0.029), oestrogen receptor negativity (P=0.0004) and c-erbB-2 overexpression (P=0.006).BRCA1 protein is postulated to function as a tumour suppressor.BRCA1 protein appears to have a significant role in both sporadic and hereditary breast cancers.

View Article: PubMed Central - PubMed

Affiliation: University Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK.

ABSTRACT
To test the hypothesis that altered expression of BRCA1 protein may play an important role in sporadic breast cancer development, 50 randomly selected primary breast cancers (frozen sections, 5 years' median follow-up) were immunolabelled with two monoclonal BRCA1 antibodies (MS110 and MS13). MS110 labelling was exclusively nuclear showing no relation to outcome or tumour pathology. Western blotting demonstrated crossreactivity, suggesting antibody nonspecificity. MS13 labelling was predominantly cytoplasmic. Intense labelling predicted decreased overall survival (P=0.012), disease-free survival (P=0.029), oestrogen receptor negativity (P=0.0004) and c-erbB-2 overexpression (P=0.006). Western blotting detected a 110 kDa molecule consistent with BRCA1 delta11b splice variant. BRCA1 protein is postulated to function as a tumour suppressor. We demonstrate cytoplasmic localisation in sporadic breast cancer suggesting excess delta11b splice variant production, reduced production of full-length BRCA1 and thus postulate reduced tumour suppressor activity. BRCA1 protein appears to have a significant role in both sporadic and hereditary breast cancers.

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Related in: MedlinePlus

Frequency histograms demonstrating the distribution of (A) MS110 labelling and (B) MS13 labelling in 50 randomly selected sporadic breast cancers.
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Related In: Results  -  Collection


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fig2: Frequency histograms demonstrating the distribution of (A) MS110 labelling and (B) MS13 labelling in 50 randomly selected sporadic breast cancers.

Mentions: Immunohistochemical data for cell lines


A role for BRCA1 in sporadic breast cancer.

Fraser JA, Reeves JR, Stanton PD, Black DM, Going JJ, Cooke TG, Bartlett JM - Br. J. Cancer (2003)

Frequency histograms demonstrating the distribution of (A) MS110 labelling and (B) MS13 labelling in 50 randomly selected sporadic breast cancers.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747570&req=5

fig2: Frequency histograms demonstrating the distribution of (A) MS110 labelling and (B) MS13 labelling in 50 randomly selected sporadic breast cancers.
Mentions: Immunohistochemical data for cell lines

Bottom Line: Intense labelling predicted decreased overall survival (P=0.012), disease-free survival (P=0.029), oestrogen receptor negativity (P=0.0004) and c-erbB-2 overexpression (P=0.006).BRCA1 protein is postulated to function as a tumour suppressor.BRCA1 protein appears to have a significant role in both sporadic and hereditary breast cancers.

View Article: PubMed Central - PubMed

Affiliation: University Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK.

ABSTRACT
To test the hypothesis that altered expression of BRCA1 protein may play an important role in sporadic breast cancer development, 50 randomly selected primary breast cancers (frozen sections, 5 years' median follow-up) were immunolabelled with two monoclonal BRCA1 antibodies (MS110 and MS13). MS110 labelling was exclusively nuclear showing no relation to outcome or tumour pathology. Western blotting demonstrated crossreactivity, suggesting antibody nonspecificity. MS13 labelling was predominantly cytoplasmic. Intense labelling predicted decreased overall survival (P=0.012), disease-free survival (P=0.029), oestrogen receptor negativity (P=0.0004) and c-erbB-2 overexpression (P=0.006). Western blotting detected a 110 kDa molecule consistent with BRCA1 delta11b splice variant. BRCA1 protein is postulated to function as a tumour suppressor. We demonstrate cytoplasmic localisation in sporadic breast cancer suggesting excess delta11b splice variant production, reduced production of full-length BRCA1 and thus postulate reduced tumour suppressor activity. BRCA1 protein appears to have a significant role in both sporadic and hereditary breast cancers.

Show MeSH
Related in: MedlinePlus