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Oesophageal squamous cell neoplasia in head and neck cancer patients: upregulation of COX-2 during carcinogenesis.

Maaser K, Däubler P, Barthel B, Heine B, von Lampe B, Stein H, Hoffmeister B, Scherer H, Scherübl H - Br. J. Cancer (2003)

Bottom Line: Patients with (previous) head and neck cancer (HNC) are at high risk for developing second squamous cell cancer of the oesophagus.COX-2 mRNA was detected in all samples, and its levels correlated positively with the immunohistochemical staining score (P<0.05).Prospective studies are needed to evaluate the chemopreventive potential of COX-2 inhibitors in this high-risk group.

View Article: PubMed Central - PubMed

Affiliation: Medical Clinic I, Gastroenterology/Infectious Disease/Rheumatology, University Hospital Benjamin Franklin, Free University of Berlin, Berlin, Germany.

ABSTRACT
Patients with (previous) head and neck cancer (HNC) are at high risk for developing second squamous cell cancer of the oesophagus. The role of cyclooxygenase-2 (COX-2) in oesophageal squamous carcinogenesis has not yet been investigated in this high-risk group. Therefore, this study examined COX-2 mRNA and protein expression in oesophageal biopsies and resected tissues of 44 HNC patients. The evaluation covered 55 oesophageal tissue samples (18 invasive oesophageal squamous cell cancers, four high- and eight low-grade dysplasias, 25 normal squamous epithelia) from the 44 patients. mRNA levels of COX-2 were measured by real-time PCR using a LightCycler. COX-2 protein expression was studied immunohistochemically and graded by a staining score. COX-2 mRNA was detected in all samples, and its levels correlated positively with the immunohistochemical staining score (P<0.05). COX-2 expression was upregulated during oesophageal squamous carcinogenesis in HNC patients, that is COX-2 expression increased significantly from normal oesophageal squamous epithelium to low- and high-grade dysplasia and finally to invasive squamous cell cancer (P<0.001). Our findings suggest that COX-2 upregulation contributes to oesophageal squamous carcinogenesis in HNC patients. Prospective studies are needed to evaluate the chemopreventive potential of COX-2 inhibitors in this high-risk group.

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COX-2 immunoreactivity in normal and neoplastic oesophageal squamous tissues of HNC patients. (A) Normal oesophageal squamous epithelium exhibits COX-2-specific staining only in cells of the basal layer (arrow); the staining score is 1. (B) Squamous cells of LGD demonstrate moderate COX-2-specific staining; the staining score is 4. (C) Poorly differentiated ESCC shows heterogeneous COX-2 staining; the staining score is 4. (A–C) Bar=100 μM. (D) Resected oesophageal tissue showing COX-2 expression that increases from normal squamous mucosa (normal) to HGD and to carcinoma (ESCC). Bar=500 μM.
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fig2: COX-2 immunoreactivity in normal and neoplastic oesophageal squamous tissues of HNC patients. (A) Normal oesophageal squamous epithelium exhibits COX-2-specific staining only in cells of the basal layer (arrow); the staining score is 1. (B) Squamous cells of LGD demonstrate moderate COX-2-specific staining; the staining score is 4. (C) Poorly differentiated ESCC shows heterogeneous COX-2 staining; the staining score is 4. (A–C) Bar=100 μM. (D) Resected oesophageal tissue showing COX-2 expression that increases from normal squamous mucosa (normal) to HGD and to carcinoma (ESCC). Bar=500 μM.

Mentions: COX-2 was mainly expressed in the basal layer of normal squamous epithelium (Figure 2AFigure 2


Oesophageal squamous cell neoplasia in head and neck cancer patients: upregulation of COX-2 during carcinogenesis.

Maaser K, Däubler P, Barthel B, Heine B, von Lampe B, Stein H, Hoffmeister B, Scherer H, Scherübl H - Br. J. Cancer (2003)

COX-2 immunoreactivity in normal and neoplastic oesophageal squamous tissues of HNC patients. (A) Normal oesophageal squamous epithelium exhibits COX-2-specific staining only in cells of the basal layer (arrow); the staining score is 1. (B) Squamous cells of LGD demonstrate moderate COX-2-specific staining; the staining score is 4. (C) Poorly differentiated ESCC shows heterogeneous COX-2 staining; the staining score is 4. (A–C) Bar=100 μM. (D) Resected oesophageal tissue showing COX-2 expression that increases from normal squamous mucosa (normal) to HGD and to carcinoma (ESCC). Bar=500 μM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747557&req=5

fig2: COX-2 immunoreactivity in normal and neoplastic oesophageal squamous tissues of HNC patients. (A) Normal oesophageal squamous epithelium exhibits COX-2-specific staining only in cells of the basal layer (arrow); the staining score is 1. (B) Squamous cells of LGD demonstrate moderate COX-2-specific staining; the staining score is 4. (C) Poorly differentiated ESCC shows heterogeneous COX-2 staining; the staining score is 4. (A–C) Bar=100 μM. (D) Resected oesophageal tissue showing COX-2 expression that increases from normal squamous mucosa (normal) to HGD and to carcinoma (ESCC). Bar=500 μM.
Mentions: COX-2 was mainly expressed in the basal layer of normal squamous epithelium (Figure 2AFigure 2

Bottom Line: Patients with (previous) head and neck cancer (HNC) are at high risk for developing second squamous cell cancer of the oesophagus.COX-2 mRNA was detected in all samples, and its levels correlated positively with the immunohistochemical staining score (P<0.05).Prospective studies are needed to evaluate the chemopreventive potential of COX-2 inhibitors in this high-risk group.

View Article: PubMed Central - PubMed

Affiliation: Medical Clinic I, Gastroenterology/Infectious Disease/Rheumatology, University Hospital Benjamin Franklin, Free University of Berlin, Berlin, Germany.

ABSTRACT
Patients with (previous) head and neck cancer (HNC) are at high risk for developing second squamous cell cancer of the oesophagus. The role of cyclooxygenase-2 (COX-2) in oesophageal squamous carcinogenesis has not yet been investigated in this high-risk group. Therefore, this study examined COX-2 mRNA and protein expression in oesophageal biopsies and resected tissues of 44 HNC patients. The evaluation covered 55 oesophageal tissue samples (18 invasive oesophageal squamous cell cancers, four high- and eight low-grade dysplasias, 25 normal squamous epithelia) from the 44 patients. mRNA levels of COX-2 were measured by real-time PCR using a LightCycler. COX-2 protein expression was studied immunohistochemically and graded by a staining score. COX-2 mRNA was detected in all samples, and its levels correlated positively with the immunohistochemical staining score (P<0.05). COX-2 expression was upregulated during oesophageal squamous carcinogenesis in HNC patients, that is COX-2 expression increased significantly from normal oesophageal squamous epithelium to low- and high-grade dysplasia and finally to invasive squamous cell cancer (P<0.001). Our findings suggest that COX-2 upregulation contributes to oesophageal squamous carcinogenesis in HNC patients. Prospective studies are needed to evaluate the chemopreventive potential of COX-2 inhibitors in this high-risk group.

Show MeSH
Related in: MedlinePlus