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On the biological relevance of MHC class II and B7 expression by tumour cells in melanoma metastases.

Bernsen MR, Håkansson L, Gustafsson B, Krysander L, Rettrup B, Ruiter D, Håkansson A - Br. J. Cancer (2003)

Bottom Line: A large number of studies have indicated that specific immune reactivity plays a crucial role in the control of malignant melanoma.In general, no or only few inflammatory cells positive for B7 were found.However, no significant correlation between B7.1 or B7.2 expression and regression of the tumour, TTP or OS was found.

View Article: PubMed Central - PubMed

Affiliation: Division of Clinical Tumour Immunology, Department of Oncology, University Hospital, Linköping, Sweden. m.bernsen@pathol.azn.nl

ABSTRACT
A large number of studies have indicated that specific immune reactivity plays a crucial role in the control of malignant melanoma. In this context, expression of MHC I, MHC II and B7 molecules by melanoma cells is seen as relevant for the immune response against the tumour. For a better understanding of the biological relevance of MHC II and B7 expression by tumour cells in metastatic melanoma, we studied the expression of these molecules in melanoma metastases in relation to the inflammatory response, regression of the tumour and survival from 27 patients treated with biochemotherapy (30 mg m(-2) Cisplatin and 250 mg m(-2) decarbazine (dimethyl-triazene-imidazole-carboxamide, DTIC) on days 1-3 i.v., and 10(7) IU IFN-alpha 2b 3 days a week s.c., q. 28d). In 19 out of 27 lesions studied, we found expression of MHC II by the tumour cells, while only in one out of 11 tumour biopsies obtained from untreated metastatic melanoma patients, MHC II expression was detected. Expression of B7.1 and B7.2 by tumour cells was found in nine out of 24 and 19 out of 24 lesions, respectively. In all cases where B7.1 expression was found, expression of B7.2 by the tumour cells was also seen. In general, no or only few inflammatory cells positive for B7 were found. Expression of MHC II by tumour cells was positively correlated with the presence of tumour-infiltrating lymphocytes, regression of the lesion, and with time to progression (TTP) and overall survival (OS) of the patient. However, no significant correlation between B7.1 or B7.2 expression and regression of the tumour, TTP or OS was found. In light of other recent findings, these data altogether do support a role as biomarker for MHC II expression by tumour cells; however, its exact immunological pathomechanism(s) remain to be established.

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Distribution of MHC II (A) and B7 (B) expression over the lesions studied.
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fig1: Distribution of MHC II (A) and B7 (B) expression over the lesions studied.

Mentions: The expression of MHC classes I and II was studied in 27 lesions from biochemotherapy-treated patients by immunohistochemical analysis. All lesions were homogeneously positive for MHC I. In contrast, a heterogeneous expression of MHC II was found between and within lesions. In eight out of these 27 lesions, the tumour cells were negative for MHC II, while in the remaining 19 lesions, 10 to more than 75% of the tumour cells were positive for MHC II (Figure 1AFigure 1


On the biological relevance of MHC class II and B7 expression by tumour cells in melanoma metastases.

Bernsen MR, Håkansson L, Gustafsson B, Krysander L, Rettrup B, Ruiter D, Håkansson A - Br. J. Cancer (2003)

Distribution of MHC II (A) and B7 (B) expression over the lesions studied.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747534&req=5

fig1: Distribution of MHC II (A) and B7 (B) expression over the lesions studied.
Mentions: The expression of MHC classes I and II was studied in 27 lesions from biochemotherapy-treated patients by immunohistochemical analysis. All lesions were homogeneously positive for MHC I. In contrast, a heterogeneous expression of MHC II was found between and within lesions. In eight out of these 27 lesions, the tumour cells were negative for MHC II, while in the remaining 19 lesions, 10 to more than 75% of the tumour cells were positive for MHC II (Figure 1AFigure 1

Bottom Line: A large number of studies have indicated that specific immune reactivity plays a crucial role in the control of malignant melanoma.In general, no or only few inflammatory cells positive for B7 were found.However, no significant correlation between B7.1 or B7.2 expression and regression of the tumour, TTP or OS was found.

View Article: PubMed Central - PubMed

Affiliation: Division of Clinical Tumour Immunology, Department of Oncology, University Hospital, Linköping, Sweden. m.bernsen@pathol.azn.nl

ABSTRACT
A large number of studies have indicated that specific immune reactivity plays a crucial role in the control of malignant melanoma. In this context, expression of MHC I, MHC II and B7 molecules by melanoma cells is seen as relevant for the immune response against the tumour. For a better understanding of the biological relevance of MHC II and B7 expression by tumour cells in metastatic melanoma, we studied the expression of these molecules in melanoma metastases in relation to the inflammatory response, regression of the tumour and survival from 27 patients treated with biochemotherapy (30 mg m(-2) Cisplatin and 250 mg m(-2) decarbazine (dimethyl-triazene-imidazole-carboxamide, DTIC) on days 1-3 i.v., and 10(7) IU IFN-alpha 2b 3 days a week s.c., q. 28d). In 19 out of 27 lesions studied, we found expression of MHC II by the tumour cells, while only in one out of 11 tumour biopsies obtained from untreated metastatic melanoma patients, MHC II expression was detected. Expression of B7.1 and B7.2 by tumour cells was found in nine out of 24 and 19 out of 24 lesions, respectively. In all cases where B7.1 expression was found, expression of B7.2 by the tumour cells was also seen. In general, no or only few inflammatory cells positive for B7 were found. Expression of MHC II by tumour cells was positively correlated with the presence of tumour-infiltrating lymphocytes, regression of the lesion, and with time to progression (TTP) and overall survival (OS) of the patient. However, no significant correlation between B7.1 or B7.2 expression and regression of the tumour, TTP or OS was found. In light of other recent findings, these data altogether do support a role as biomarker for MHC II expression by tumour cells; however, its exact immunological pathomechanism(s) remain to be established.

Show MeSH
Related in: MedlinePlus