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Irradiation stability and cytotoxicity of gold nanoparticles for radiotherapy.

Zhang XD, Guo ML, Wu HY, Sun YM, Ding YQ, Feng X, Zhang LA - Int J Nanomedicine (2009)

Bottom Line: Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy.Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability.Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, People's Republic of China.

ABSTRACT
Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy. If gold nanoparticles are shown to have good irradiation stability and biocompatibility, they would play an important role in radiotherapy. In this work, we investigated irradiation effects of gold nanoparticles under 2-10 kR gamma irradiation and cytotoxicity of gold nanoparticles with human K562 cells by using Cell Titre-Glo luminescent cell viability assay. The results revealed that gamma irradiation had not induced any obvious instability and size variations in gold nanoparticles. We found that gold nanoparticles showed excellent radiation hardness with an absorbed dose conversation factor of 9.491 rad/R. Meanwhile, the surface plasmon resonance of gold nanoparticles was enhanced obviously after 2-10 kR gamma irradiation. Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability. Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

Show MeSH
The relative and normalized SPR enhancement of gold NPs dependent on irradiated dose.Abbreviations: NPs, nanoparticles; SPR, surface plasmon resonance.
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f3-ijn-4-165: The relative and normalized SPR enhancement of gold NPs dependent on irradiated dose.Abbreviations: NPs, nanoparticles; SPR, surface plasmon resonance.

Mentions: It is worth noting that the SPR peak of gold NPs is enhanced obviously after gamma irradiation. The enhancement of SPR is very obvious at the dose of 4 kR and the enhanced effect is gradually stable above 6 kR. Both groups perform good reproducibility. Besides, we have also investigated the optical effect of sodium citrate solution under 2–10 kR irradiation, which has not shown any absorption in the 500–800 nm. This indicates that the enhanced absorption is from the SPR of gold NPs, not the dissolved citrate. Figure 3 shows the enhanced effect of SPR in group 1 of Figure 2. It can be observed that the absorbance of SPR is increased from 1.56 of nonirradiation to 2.23 of 40 kR irradiation, and stabilized to 2.0 in the range of 6–10 kR. After normalization, the enhancements of SPR intensity are 10.9%, 40%, 29.1%, 25.5%, and 23.1%, which correspond to 2, 4, 6, 8, and 10 kR irradiated doses, respectively. We notice that the high dose irradiations of 6–10 kR have not induced obvious variation in SPR, which indicates that 4 kR may be the optimal energy for SPR modification of gold NPs.


Irradiation stability and cytotoxicity of gold nanoparticles for radiotherapy.

Zhang XD, Guo ML, Wu HY, Sun YM, Ding YQ, Feng X, Zhang LA - Int J Nanomedicine (2009)

The relative and normalized SPR enhancement of gold NPs dependent on irradiated dose.Abbreviations: NPs, nanoparticles; SPR, surface plasmon resonance.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747351&req=5

f3-ijn-4-165: The relative and normalized SPR enhancement of gold NPs dependent on irradiated dose.Abbreviations: NPs, nanoparticles; SPR, surface plasmon resonance.
Mentions: It is worth noting that the SPR peak of gold NPs is enhanced obviously after gamma irradiation. The enhancement of SPR is very obvious at the dose of 4 kR and the enhanced effect is gradually stable above 6 kR. Both groups perform good reproducibility. Besides, we have also investigated the optical effect of sodium citrate solution under 2–10 kR irradiation, which has not shown any absorption in the 500–800 nm. This indicates that the enhanced absorption is from the SPR of gold NPs, not the dissolved citrate. Figure 3 shows the enhanced effect of SPR in group 1 of Figure 2. It can be observed that the absorbance of SPR is increased from 1.56 of nonirradiation to 2.23 of 40 kR irradiation, and stabilized to 2.0 in the range of 6–10 kR. After normalization, the enhancements of SPR intensity are 10.9%, 40%, 29.1%, 25.5%, and 23.1%, which correspond to 2, 4, 6, 8, and 10 kR irradiated doses, respectively. We notice that the high dose irradiations of 6–10 kR have not induced obvious variation in SPR, which indicates that 4 kR may be the optimal energy for SPR modification of gold NPs.

Bottom Line: Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy.Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability.Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, People's Republic of China.

ABSTRACT
Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy. If gold nanoparticles are shown to have good irradiation stability and biocompatibility, they would play an important role in radiotherapy. In this work, we investigated irradiation effects of gold nanoparticles under 2-10 kR gamma irradiation and cytotoxicity of gold nanoparticles with human K562 cells by using Cell Titre-Glo luminescent cell viability assay. The results revealed that gamma irradiation had not induced any obvious instability and size variations in gold nanoparticles. We found that gold nanoparticles showed excellent radiation hardness with an absorbed dose conversation factor of 9.491 rad/R. Meanwhile, the surface plasmon resonance of gold nanoparticles was enhanced obviously after 2-10 kR gamma irradiation. Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability. Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

Show MeSH