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Irradiation stability and cytotoxicity of gold nanoparticles for radiotherapy.

Zhang XD, Guo ML, Wu HY, Sun YM, Ding YQ, Feng X, Zhang LA - Int J Nanomedicine (2009)

Bottom Line: Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy.Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability.Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, People's Republic of China.

ABSTRACT
Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy. If gold nanoparticles are shown to have good irradiation stability and biocompatibility, they would play an important role in radiotherapy. In this work, we investigated irradiation effects of gold nanoparticles under 2-10 kR gamma irradiation and cytotoxicity of gold nanoparticles with human K562 cells by using Cell Titre-Glo luminescent cell viability assay. The results revealed that gamma irradiation had not induced any obvious instability and size variations in gold nanoparticles. We found that gold nanoparticles showed excellent radiation hardness with an absorbed dose conversation factor of 9.491 rad/R. Meanwhile, the surface plasmon resonance of gold nanoparticles was enhanced obviously after 2-10 kR gamma irradiation. Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability. Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

Show MeSH
The optical absorption of the nonirradiated, 2, 4, 6, 8, and 10 kR irradiated gold NPs. The gold concentration of group 2 is half of group 1.Abbreviation: NPs, nanoparticles.
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f2-ijn-4-165: The optical absorption of the nonirradiated, 2, 4, 6, 8, and 10 kR irradiated gold NPs. The gold concentration of group 2 is half of group 1.Abbreviation: NPs, nanoparticles.

Mentions: Figure 2 gives the optical absorption of nonirradiated gold NPs, 2, 4, 6, 8, and 10 kR irradiated gold NPs. The SPR band of 521 nm has been observed in nonirradiated gold NPs, and the peak is not shifted after gamma irradiation. Indeed, the so slight size variation should not induce the obvious shift of SPR peak. Mie theory is the exact solution to Maxwell’s electromagnetic field equations for a plane wave interacting with a homogenous sphere of radius with the same dielectric constant as bulk metal.30 The size of gold NPs is in good agreement with the results calculated by Mie theory. Group 2 is added in order to illustrate the validity and reproducibility of results, in which the concentration of gold NPs is half of group 1. The sole SPR peak in the spectra indicates that gamma irradiation has not caused obvious defects in gold NPs. The full width at half-maximum of the SPR band is about 50 nm, and has no obvious variation after gamma irradiation, which also indicates that gold NPs are very stable after gamma irradiation.


Irradiation stability and cytotoxicity of gold nanoparticles for radiotherapy.

Zhang XD, Guo ML, Wu HY, Sun YM, Ding YQ, Feng X, Zhang LA - Int J Nanomedicine (2009)

The optical absorption of the nonirradiated, 2, 4, 6, 8, and 10 kR irradiated gold NPs. The gold concentration of group 2 is half of group 1.Abbreviation: NPs, nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747351&req=5

f2-ijn-4-165: The optical absorption of the nonirradiated, 2, 4, 6, 8, and 10 kR irradiated gold NPs. The gold concentration of group 2 is half of group 1.Abbreviation: NPs, nanoparticles.
Mentions: Figure 2 gives the optical absorption of nonirradiated gold NPs, 2, 4, 6, 8, and 10 kR irradiated gold NPs. The SPR band of 521 nm has been observed in nonirradiated gold NPs, and the peak is not shifted after gamma irradiation. Indeed, the so slight size variation should not induce the obvious shift of SPR peak. Mie theory is the exact solution to Maxwell’s electromagnetic field equations for a plane wave interacting with a homogenous sphere of radius with the same dielectric constant as bulk metal.30 The size of gold NPs is in good agreement with the results calculated by Mie theory. Group 2 is added in order to illustrate the validity and reproducibility of results, in which the concentration of gold NPs is half of group 1. The sole SPR peak in the spectra indicates that gamma irradiation has not caused obvious defects in gold NPs. The full width at half-maximum of the SPR band is about 50 nm, and has no obvious variation after gamma irradiation, which also indicates that gold NPs are very stable after gamma irradiation.

Bottom Line: Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy.Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability.Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, People's Republic of China.

ABSTRACT
Gold nanoparticles are promising as a kind of novel radiosensitizer in radiotherapy. If gold nanoparticles are shown to have good irradiation stability and biocompatibility, they would play an important role in radiotherapy. In this work, we investigated irradiation effects of gold nanoparticles under 2-10 kR gamma irradiation and cytotoxicity of gold nanoparticles with human K562 cells by using Cell Titre-Glo luminescent cell viability assay. The results revealed that gamma irradiation had not induced any obvious instability and size variations in gold nanoparticles. We found that gold nanoparticles showed excellent radiation hardness with an absorbed dose conversation factor of 9.491 rad/R. Meanwhile, the surface plasmon resonance of gold nanoparticles was enhanced obviously after 2-10 kR gamma irradiation. Subsequently, cytotoxicity tests indicated that the extremely high concentration of gold nanoparticles could cause a sharp decrease in K562 cell viability, while the low concentration of gold nanoparticles had no obvious influence on the cell viability. Our results revealed that gold nanoparticles were stable under high-energy ray irradiation and showed concentration-dependent cytotoxicity.

Show MeSH