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Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA.

Moselhy J, Vira T, Liu FF, Wu XY - Int J Nanomedicine (2009)

Bottom Line: Quaternized P[NIPAM/DMAEMAQ] TCNGs did not undergo sharp VPT up to 50 degrees C.The VPT of P[NIPAM/DMAEMAQ]/sasDNA diminished in magnitude with increasing W(p)/W(d) suggesting greater compaction of the polyplexes.The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada M5S 3M2.

ABSTRACT
Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacrylamide (NIPAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and quaternary alkyl ammonium halide salts of DMAEMA (DMAEMAQ) were synthesized by dispersion polymerization technique. The thermoresponsive properties of TCNGs and TCNG-salmon sperm DNA (sasDNA) polyplexes were characterized in aqueous media of various pH and ionic strength. P[NIPAM] and P[NIPAM/DMAEMA] TCNGs exhibited sharp volume phase transition (VPT) in water at critical temperatures (T(c)) of 32 degrees C and 36 degrees C, respectively. Quaternized P[NIPAM/DMAEMAQ] TCNGs did not undergo sharp VPT up to 50 degrees C. The VPT of uncomplexed TCNGs were sensitive to the ionic composition and ionic strength of salts in solution, but were insensitive to pH in the range 5.0 to 7.4. The VPT of P[NIPAM/DMAEMAQ]/sasDNA diminished in magnitude with increasing W(p)/W(d) suggesting greater compaction of the polyplexes. The distinct phase-transition properties of P[NIPAM/DMAEMA]/sasDNA and P[NIPAM/DMAEMAQ]/sasDNA polyplexes were attributed to the condensing capability of polycations and to differences in the spatial distribution of structural charges in quaternized and nonquaternized networks. The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.

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Dependence of zeta-potential of NDQPRI6/sasDNA and NDQPI6/sasDNA on Wp/Wd in DDIW. Numbers in parentheses are zeta-potentials of respective TCNGs in absence of DNA (Wp/Wd = ∞).
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f10-ijn-4-153: Dependence of zeta-potential of NDQPRI6/sasDNA and NDQPI6/sasDNA on Wp/Wd in DDIW. Numbers in parentheses are zeta-potentials of respective TCNGs in absence of DNA (Wp/Wd = ∞).

Mentions: Figure 10 shows the change in ζ of NDQPI6/sas DNA and NDQPRI6/sasDNA polyplexes as a function of Wp/Wd. For both of these polyplexes, the slope of the curve decreases substantially at around Wp/Wd = 2, suggesting the saturation of DNA-binding capability of the TCNG near this weight ratio. This supports particle size data that showed little change in temperature response of the polyplexes above this weight ratio.


Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA.

Moselhy J, Vira T, Liu FF, Wu XY - Int J Nanomedicine (2009)

Dependence of zeta-potential of NDQPRI6/sasDNA and NDQPI6/sasDNA on Wp/Wd in DDIW. Numbers in parentheses are zeta-potentials of respective TCNGs in absence of DNA (Wp/Wd = ∞).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747350&req=5

f10-ijn-4-153: Dependence of zeta-potential of NDQPRI6/sasDNA and NDQPI6/sasDNA on Wp/Wd in DDIW. Numbers in parentheses are zeta-potentials of respective TCNGs in absence of DNA (Wp/Wd = ∞).
Mentions: Figure 10 shows the change in ζ of NDQPI6/sas DNA and NDQPRI6/sasDNA polyplexes as a function of Wp/Wd. For both of these polyplexes, the slope of the curve decreases substantially at around Wp/Wd = 2, suggesting the saturation of DNA-binding capability of the TCNG near this weight ratio. This supports particle size data that showed little change in temperature response of the polyplexes above this weight ratio.

Bottom Line: Quaternized P[NIPAM/DMAEMAQ] TCNGs did not undergo sharp VPT up to 50 degrees C.The VPT of P[NIPAM/DMAEMAQ]/sasDNA diminished in magnitude with increasing W(p)/W(d) suggesting greater compaction of the polyplexes.The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada M5S 3M2.

ABSTRACT
Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacrylamide (NIPAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and quaternary alkyl ammonium halide salts of DMAEMA (DMAEMAQ) were synthesized by dispersion polymerization technique. The thermoresponsive properties of TCNGs and TCNG-salmon sperm DNA (sasDNA) polyplexes were characterized in aqueous media of various pH and ionic strength. P[NIPAM] and P[NIPAM/DMAEMA] TCNGs exhibited sharp volume phase transition (VPT) in water at critical temperatures (T(c)) of 32 degrees C and 36 degrees C, respectively. Quaternized P[NIPAM/DMAEMAQ] TCNGs did not undergo sharp VPT up to 50 degrees C. The VPT of uncomplexed TCNGs were sensitive to the ionic composition and ionic strength of salts in solution, but were insensitive to pH in the range 5.0 to 7.4. The VPT of P[NIPAM/DMAEMAQ]/sasDNA diminished in magnitude with increasing W(p)/W(d) suggesting greater compaction of the polyplexes. The distinct phase-transition properties of P[NIPAM/DMAEMA]/sasDNA and P[NIPAM/DMAEMAQ]/sasDNA polyplexes were attributed to the condensing capability of polycations and to differences in the spatial distribution of structural charges in quaternized and nonquaternized networks. The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.

Show MeSH
Related in: MedlinePlus