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The use of superparamagnetic nanoparticles for prosthetic biofilm prevention.

Taylor EN, Webster TJ - Int J Nanomedicine (2009)

Bottom Line: Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours.Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours.Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine Laboratories, Division of Engineering, and Department of Orthopaedics, Brown University, Providence, RI 02912, USA.

ABSTRACT
As with all surgical procedures, implantation comes with the added risk of infection. The goal of this in vitro study was to explore the use of superparamagnetic iron oxide nanoparticles (SPION) as a multifunctional platform to prevent biofilm formation. Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours. Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours. Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

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Percent of dead bacteria quantified using fluorescence microscopy after 48 hours. Total fluorescent area of bacteria cultures was determined for both green (live) and red (dead) regions and the average dead bacteria staining area for each is reported. A significant increase in dead cells was found for high superparamagnetic iron oxide nanoparticle doses compared to controls (0 mg/ml), but not for the 10 μg/ml or supernatant alone conditions.Notes: All P values denoted with *are significant at the 5% level compared to controls and error bars are standard error of the mean (N = 3).
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f5-ijn-4-145: Percent of dead bacteria quantified using fluorescence microscopy after 48 hours. Total fluorescent area of bacteria cultures was determined for both green (live) and red (dead) regions and the average dead bacteria staining area for each is reported. A significant increase in dead cells was found for high superparamagnetic iron oxide nanoparticle doses compared to controls (0 mg/ml), but not for the 10 μg/ml or supernatant alone conditions.Notes: All P values denoted with *are significant at the 5% level compared to controls and error bars are standard error of the mean (N = 3).

Mentions: Fluorescence microscopy results demonstrated a higher percentage of dead bacteria when exposed to SPION after 48 hours (Figures 5–6). TFA quantification provided, for the first time, evidence that bacteria death increased in the presence of all SPION dosages of interest to this study (Figure 5). A significant increase in bacteria death occurred at SPION concentrations of 2 mg/mL, 1 mg/mL, and 100 μg/mL compared to controls.


The use of superparamagnetic nanoparticles for prosthetic biofilm prevention.

Taylor EN, Webster TJ - Int J Nanomedicine (2009)

Percent of dead bacteria quantified using fluorescence microscopy after 48 hours. Total fluorescent area of bacteria cultures was determined for both green (live) and red (dead) regions and the average dead bacteria staining area for each is reported. A significant increase in dead cells was found for high superparamagnetic iron oxide nanoparticle doses compared to controls (0 mg/ml), but not for the 10 μg/ml or supernatant alone conditions.Notes: All P values denoted with *are significant at the 5% level compared to controls and error bars are standard error of the mean (N = 3).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747349&req=5

f5-ijn-4-145: Percent of dead bacteria quantified using fluorescence microscopy after 48 hours. Total fluorescent area of bacteria cultures was determined for both green (live) and red (dead) regions and the average dead bacteria staining area for each is reported. A significant increase in dead cells was found for high superparamagnetic iron oxide nanoparticle doses compared to controls (0 mg/ml), but not for the 10 μg/ml or supernatant alone conditions.Notes: All P values denoted with *are significant at the 5% level compared to controls and error bars are standard error of the mean (N = 3).
Mentions: Fluorescence microscopy results demonstrated a higher percentage of dead bacteria when exposed to SPION after 48 hours (Figures 5–6). TFA quantification provided, for the first time, evidence that bacteria death increased in the presence of all SPION dosages of interest to this study (Figure 5). A significant increase in bacteria death occurred at SPION concentrations of 2 mg/mL, 1 mg/mL, and 100 μg/mL compared to controls.

Bottom Line: Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours.Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours.Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine Laboratories, Division of Engineering, and Department of Orthopaedics, Brown University, Providence, RI 02912, USA.

ABSTRACT
As with all surgical procedures, implantation comes with the added risk of infection. The goal of this in vitro study was to explore the use of superparamagnetic iron oxide nanoparticles (SPION) as a multifunctional platform to prevent biofilm formation. Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours. Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours. Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

Show MeSH
Related in: MedlinePlus