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The use of superparamagnetic nanoparticles for prosthetic biofilm prevention.

Taylor EN, Webster TJ - Int J Nanomedicine (2009)

Bottom Line: Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours.Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours.Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine Laboratories, Division of Engineering, and Department of Orthopaedics, Brown University, Providence, RI 02912, USA.

ABSTRACT
As with all surgical procedures, implantation comes with the added risk of infection. The goal of this in vitro study was to explore the use of superparamagnetic iron oxide nanoparticles (SPION) as a multifunctional platform to prevent biofilm formation. Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours. Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours. Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

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Decreased S. epidermidis numbers in the presence of superparamagnetic iron oxide nanoparticles. Cells were inoculated at 3 × 106 cells per well and were cultured for up to 48 hours. Data was obtained using optical density readings and each bar represents the average of 24 readings. Bacteria density decreased for doses as low as 100 μg/ml compared to no particles (0 mg/ml) at all time points assessed. The lowest dose, 10 μg/ml, was not significantly different than controls (no particles).Notes: All P values assessed at the 1% level are denoted **and error bars indicate standard error of the mean (N = 3).
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f4-ijn-4-145: Decreased S. epidermidis numbers in the presence of superparamagnetic iron oxide nanoparticles. Cells were inoculated at 3 × 106 cells per well and were cultured for up to 48 hours. Data was obtained using optical density readings and each bar represents the average of 24 readings. Bacteria density decreased for doses as low as 100 μg/ml compared to no particles (0 mg/ml) at all time points assessed. The lowest dose, 10 μg/ml, was not significantly different than controls (no particles).Notes: All P values assessed at the 1% level are denoted **and error bars indicate standard error of the mean (N = 3).

Mentions: Optical density studies indicated progressively decreased S. epidermidis density at all time points (12–48 hours) when cultured with 100 μg/mL, 1 mg/mL, and 2 mg/mL (Figure 4). This is in contrast with the low SPION concentration of 10 μg/mL for which no significant difference was observed compared to controls (no particles). The SPION supernatant control (possibly containing ethanol and iron salt) did not cause a decrease in bacteria density compared to no particle controls, thus, providing evidence that the iron oxide nanoparticles (not other chemicals used/created when fabricating such particles) decreased bacteria number.


The use of superparamagnetic nanoparticles for prosthetic biofilm prevention.

Taylor EN, Webster TJ - Int J Nanomedicine (2009)

Decreased S. epidermidis numbers in the presence of superparamagnetic iron oxide nanoparticles. Cells were inoculated at 3 × 106 cells per well and were cultured for up to 48 hours. Data was obtained using optical density readings and each bar represents the average of 24 readings. Bacteria density decreased for doses as low as 100 μg/ml compared to no particles (0 mg/ml) at all time points assessed. The lowest dose, 10 μg/ml, was not significantly different than controls (no particles).Notes: All P values assessed at the 1% level are denoted **and error bars indicate standard error of the mean (N = 3).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747349&req=5

f4-ijn-4-145: Decreased S. epidermidis numbers in the presence of superparamagnetic iron oxide nanoparticles. Cells were inoculated at 3 × 106 cells per well and were cultured for up to 48 hours. Data was obtained using optical density readings and each bar represents the average of 24 readings. Bacteria density decreased for doses as low as 100 μg/ml compared to no particles (0 mg/ml) at all time points assessed. The lowest dose, 10 μg/ml, was not significantly different than controls (no particles).Notes: All P values assessed at the 1% level are denoted **and error bars indicate standard error of the mean (N = 3).
Mentions: Optical density studies indicated progressively decreased S. epidermidis density at all time points (12–48 hours) when cultured with 100 μg/mL, 1 mg/mL, and 2 mg/mL (Figure 4). This is in contrast with the low SPION concentration of 10 μg/mL for which no significant difference was observed compared to controls (no particles). The SPION supernatant control (possibly containing ethanol and iron salt) did not cause a decrease in bacteria density compared to no particle controls, thus, providing evidence that the iron oxide nanoparticles (not other chemicals used/created when fabricating such particles) decreased bacteria number.

Bottom Line: Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours.Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours.Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine Laboratories, Division of Engineering, and Department of Orthopaedics, Brown University, Providence, RI 02912, USA.

ABSTRACT
As with all surgical procedures, implantation comes with the added risk of infection. The goal of this in vitro study was to explore the use of superparamagnetic iron oxide nanoparticles (SPION) as a multifunctional platform to prevent biofilm formation. Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours. Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours. Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

Show MeSH
Related in: MedlinePlus