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The use of superparamagnetic nanoparticles for prosthetic biofilm prevention.

Taylor EN, Webster TJ - Int J Nanomedicine (2009)

Bottom Line: Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours.Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours.Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine Laboratories, Division of Engineering, and Department of Orthopaedics, Brown University, Providence, RI 02912, USA.

ABSTRACT
As with all surgical procedures, implantation comes with the added risk of infection. The goal of this in vitro study was to explore the use of superparamagnetic iron oxide nanoparticles (SPION) as a multifunctional platform to prevent biofilm formation. Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours. Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours. Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

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Related in: MedlinePlus

X-ray diffraction spectrum of the superparamagnetic iron oxide nanoparticle (SPION). Magnetite (Fe3O4) peaks (M) are shown confirming the crystalline phases of the SPION.
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f2-ijn-4-145: X-ray diffraction spectrum of the superparamagnetic iron oxide nanoparticle (SPION). Magnetite (Fe3O4) peaks (M) are shown confirming the crystalline phases of the SPION.

Mentions: The SPION synthesized were superparamagnetic with very low hysteresis and a saturation magnetization around 30 electromagnetic units per gram (emu/g) (Figure 1). Diffraction peaks acquired matched with Fe3O4 (Figure 2). Electron microscopy visualization demonstrated that uncoated nanoparticles had a cubic structure with a maximum particle size of 18 nm and an average size of about 8 nm (Figure 3).


The use of superparamagnetic nanoparticles for prosthetic biofilm prevention.

Taylor EN, Webster TJ - Int J Nanomedicine (2009)

X-ray diffraction spectrum of the superparamagnetic iron oxide nanoparticle (SPION). Magnetite (Fe3O4) peaks (M) are shown confirming the crystalline phases of the SPION.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747349&req=5

f2-ijn-4-145: X-ray diffraction spectrum of the superparamagnetic iron oxide nanoparticle (SPION). Magnetite (Fe3O4) peaks (M) are shown confirming the crystalline phases of the SPION.
Mentions: The SPION synthesized were superparamagnetic with very low hysteresis and a saturation magnetization around 30 electromagnetic units per gram (emu/g) (Figure 1). Diffraction peaks acquired matched with Fe3O4 (Figure 2). Electron microscopy visualization demonstrated that uncoated nanoparticles had a cubic structure with a maximum particle size of 18 nm and an average size of about 8 nm (Figure 3).

Bottom Line: Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours.Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours.Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

View Article: PubMed Central - PubMed

Affiliation: Nanomedicine Laboratories, Division of Engineering, and Department of Orthopaedics, Brown University, Providence, RI 02912, USA.

ABSTRACT
As with all surgical procedures, implantation comes with the added risk of infection. The goal of this in vitro study was to explore the use of superparamagnetic iron oxide nanoparticles (SPION) as a multifunctional platform to prevent biofilm formation. Results showed for the first time decreased Staphylococcus epidermidis numbers when exposed to 100 microg/ml of SPION for 12 hours and this trend continued for up to 48 hours. Prevention of colony assembly, a prerequisite to biofilm formation, was also observed at lower SPION dosages of 10 microg/ml after 12 hours. Coupled with previous studies demonstrating enhanced bone cell functions in the presence of the same concentration of SPION, the present results provided much promise for the use of SPION for numerous anti-infection orthopedic applications.

Show MeSH
Related in: MedlinePlus