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Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity.

Prakash D, Behari J - Int J Nanomedicine (2009)

Bottom Line: In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted.As a countermeasure to the above, the effect of PEMF and HAp application were examined.Ca (p > 0.01).

View Article: PubMed Central - PubMed

Affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi--110067, India.

ABSTRACT
The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p < 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p < 0.1), Ca (p < 0.1), P (p < 0.5), type I collagen (p < 0.1), and ALP activity (p < 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p < 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p < 0.02), Ca (p < 0.05), P (p < 0.05), type I collagen (p < 0.02), and ALP activity (p > 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p > 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p > 0.01). Ca (p > 0.01). P (p > 0.01). type I collagen (p > 0.01). and ALP activity (p > 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p > 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.

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(A) Total type I collagen (μg/mg of dry weight) in femur. (B) Total type I collagen (μg/mg of dry weight) in tibia. Nonsignificant increase in HLS and HLS + P.Notes: *Indicates significant difference between: 1) control and HLS, 2) HLS and HLS + N, 3) HLS and HLS + P + N.Abbreviations: HLS, hind-limb suspension; HLS + N, hind-limb suspension + nanoparticle (Si-HAp); HLS + P, hind-limb suspension + PEMF; HLS + P + N, hind-limb suspension + PEMF + nanoparticle; PEMF, pulsed electromagnetic field.
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f14-ijn-4-133: (A) Total type I collagen (μg/mg of dry weight) in femur. (B) Total type I collagen (μg/mg of dry weight) in tibia. Nonsignificant increase in HLS and HLS + P.Notes: *Indicates significant difference between: 1) control and HLS, 2) HLS and HLS + N, 3) HLS and HLS + P + N.Abbreviations: HLS, hind-limb suspension; HLS + N, hind-limb suspension + nanoparticle (Si-HAp); HLS + P, hind-limb suspension + PEMF; HLS + P + N, hind-limb suspension + PEMF + nanoparticle; PEMF, pulsed electromagnetic field.

Mentions: Sirius Red, a strong anionic dye, stains collagen by reacting via its sulphonic acid groups, with the basic group present in collagen molecule. Collagen extracted with acetic acid and pepsin from bone samples of different groups was analyzed. The detailed results of type I collagen in the femur and tibia for different rat groups are presented in Figure 14 a, b.


Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity.

Prakash D, Behari J - Int J Nanomedicine (2009)

(A) Total type I collagen (μg/mg of dry weight) in femur. (B) Total type I collagen (μg/mg of dry weight) in tibia. Nonsignificant increase in HLS and HLS + P.Notes: *Indicates significant difference between: 1) control and HLS, 2) HLS and HLS + N, 3) HLS and HLS + P + N.Abbreviations: HLS, hind-limb suspension; HLS + N, hind-limb suspension + nanoparticle (Si-HAp); HLS + P, hind-limb suspension + PEMF; HLS + P + N, hind-limb suspension + PEMF + nanoparticle; PEMF, pulsed electromagnetic field.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747348&req=5

f14-ijn-4-133: (A) Total type I collagen (μg/mg of dry weight) in femur. (B) Total type I collagen (μg/mg of dry weight) in tibia. Nonsignificant increase in HLS and HLS + P.Notes: *Indicates significant difference between: 1) control and HLS, 2) HLS and HLS + N, 3) HLS and HLS + P + N.Abbreviations: HLS, hind-limb suspension; HLS + N, hind-limb suspension + nanoparticle (Si-HAp); HLS + P, hind-limb suspension + PEMF; HLS + P + N, hind-limb suspension + PEMF + nanoparticle; PEMF, pulsed electromagnetic field.
Mentions: Sirius Red, a strong anionic dye, stains collagen by reacting via its sulphonic acid groups, with the basic group present in collagen molecule. Collagen extracted with acetic acid and pepsin from bone samples of different groups was analyzed. The detailed results of type I collagen in the femur and tibia for different rat groups are presented in Figure 14 a, b.

Bottom Line: In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted.As a countermeasure to the above, the effect of PEMF and HAp application were examined.Ca (p > 0.01).

View Article: PubMed Central - PubMed

Affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi--110067, India.

ABSTRACT
The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p < 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p < 0.1), Ca (p < 0.1), P (p < 0.5), type I collagen (p < 0.1), and ALP activity (p < 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p < 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p < 0.02), Ca (p < 0.05), P (p < 0.05), type I collagen (p < 0.02), and ALP activity (p > 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p > 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p > 0.01). Ca (p > 0.01). P (p > 0.01). type I collagen (p > 0.01). and ALP activity (p > 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p > 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.

Show MeSH
Related in: MedlinePlus