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Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity.

Prakash D, Behari J - Int J Nanomedicine (2009)

Bottom Line: In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted.As a countermeasure to the above, the effect of PEMF and HAp application were examined.Ca (p > 0.01).

View Article: PubMed Central - PubMed

Affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi--110067, India.

ABSTRACT
The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p < 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p < 0.1), Ca (p < 0.1), P (p < 0.5), type I collagen (p < 0.1), and ALP activity (p < 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p < 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p < 0.02), Ca (p < 0.05), P (p < 0.05), type I collagen (p < 0.02), and ALP activity (p > 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p > 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p > 0.01). Ca (p > 0.01). P (p > 0.01). type I collagen (p > 0.01). and ALP activity (p > 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p > 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.

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(A) SEM image of cortical part of tibia (HLS + P). (B) SEM image of cortical part of tibia (HLS + N + P).Abbreviations: HLS + P, hind-limb suspension + PEMF; HLS + N + P, hind-limb suspension + nanoparticle + PEMF; PEMF, pulsed electromagnetic field; SEM, scanning electron microscope.
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f12-ijn-4-133: (A) SEM image of cortical part of tibia (HLS + P). (B) SEM image of cortical part of tibia (HLS + N + P).Abbreviations: HLS + P, hind-limb suspension + PEMF; HLS + N + P, hind-limb suspension + nanoparticle + PEMF; PEMF, pulsed electromagnetic field; SEM, scanning electron microscope.

Mentions: SEM was performed in all groups of bone samples to evaluate the changes in cortex and cancellous part of bone. Transverse section of femur and tibia bone of control, HLS, and treated (HLS + P, HLS + N, HLS + P + N) rats were analyzed. More compactness in cancellous part and mineral deposition in control and treated bone were found compared to HLS bone (Figures 5–8). Similar results were also obtained in cortical thickness among various rat groups (Figures 9–12). It was observed that bone marrow was attached to the cortex in treated groups was visible in normal controls. There was a deposition of white matter in HLS + P + N group rats, which may be a deposit of hydroxyapatite particles.


Synergistic role of hydroxyapatite nanoparticles and pulsed electromagnetic field therapy to prevent bone loss in rats following exposure to simulated microgravity.

Prakash D, Behari J - Int J Nanomedicine (2009)

(A) SEM image of cortical part of tibia (HLS + P). (B) SEM image of cortical part of tibia (HLS + N + P).Abbreviations: HLS + P, hind-limb suspension + PEMF; HLS + N + P, hind-limb suspension + nanoparticle + PEMF; PEMF, pulsed electromagnetic field; SEM, scanning electron microscope.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747348&req=5

f12-ijn-4-133: (A) SEM image of cortical part of tibia (HLS + P). (B) SEM image of cortical part of tibia (HLS + N + P).Abbreviations: HLS + P, hind-limb suspension + PEMF; HLS + N + P, hind-limb suspension + nanoparticle + PEMF; PEMF, pulsed electromagnetic field; SEM, scanning electron microscope.
Mentions: SEM was performed in all groups of bone samples to evaluate the changes in cortex and cancellous part of bone. Transverse section of femur and tibia bone of control, HLS, and treated (HLS + P, HLS + N, HLS + P + N) rats were analyzed. More compactness in cancellous part and mineral deposition in control and treated bone were found compared to HLS bone (Figures 5–8). Similar results were also obtained in cortical thickness among various rat groups (Figures 9–12). It was observed that bone marrow was attached to the cortex in treated groups was visible in normal controls. There was a deposition of white matter in HLS + P + N group rats, which may be a deposit of hydroxyapatite particles.

Bottom Line: In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted.As a countermeasure to the above, the effect of PEMF and HAp application were examined.Ca (p > 0.01).

View Article: PubMed Central - PubMed

Affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi--110067, India.

ABSTRACT
The purpose of the present study was to use capacitive coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We used the hind-limb suspension (HLS) rat model to simulate microgravity-induced bone losses for 45 days. In order to compare the resulting changes, mineralogical (bone mineral density [BMD], calcium [Ca], and phosphorus [P]), biochemical (osteocalcin, alkaline phosphatase [ALP], and type I collagen), and histological (scanning electron microscopy) parameters were adopted. As a countermeasure to the above, the effect of PEMF and HAp application were examined. Three-month-old female Wistar rats were randomly divided into control (n = 8), HLS (n = 8), HLS with PEMF (n = 8), HLS with HAp nanoparticles (n = 8), and HLS with HAp and PEMF (n = 8). We observed: 1) significant decrease (p < 0.01) in BMD, Ca, P, type I collagen, and ALP activity in femur and tibia in hind-limb bone and serum osteocalcin in HLS rats as compared with the ground control. 2) Nonsignificant increase in BMD (p < 0.1), Ca (p < 0.1), P (p < 0.5), type I collagen (p < 0.1), and ALP activity (p < 0.5) in femur and tibia in hind-limb bone and serum osteocalcin (p < 0.5) in HLS + PEMF rats compared with HLS rats. 3) Significant increase in BMD (p < 0.02), Ca (p < 0.05), P (p < 0.05), type I collagen (p < 0.02), and ALP activity (p > 0.02) in femur and tibia in hind-limb bone with a nonsignificant increase in serum osteocalcin (p > 0.1) in HLS + HAp rats compared to HLS rats. 4) Significant increase in BMD (p > 0.01). Ca (p > 0.01). P (p > 0.01). type I collagen (p > 0.01). and ALP activity (p > 0.01) in femur and tibia in hind-limb bone and serum osteocalcin (p > 0.02) were also observed. Results suggest that a combination of low level PEMF and Hap nanoparticles has potential to control bone loss induced by simulated microgravity.

Show MeSH
Related in: MedlinePlus