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Phylogenetic analyses reveal monophyletic origin of the ergot alkaloid gene dmaW in fungi.

Liu M, Panaccione DG, Schardl CL - Evol. Bioinform. Online (2009)

Bottom Line: The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway.Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi.Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

View Article: PubMed Central - PubMed

Affiliation: 201 F Plant Science Bldg, University of Kentucky, Lexington, KY 40546, USA.

ABSTRACT
Ergot alkaloids are indole-derived mycotoxins that are important in agriculture and medicine. Ergot alkaloids are produced by a few representatives of two distantly related fungal lineages, the Clavicipitaceae and the Trichocomaceae. Comparison of the ergot alkaloid gene clusters from these two lineages revealed differences in the relative positions and orientations of several genes. The question arose: is ergot alkaloid biosynthetic capability from a common origin? We used a molecular phylogenetic approach to gain insights into the evolution of ergot alkaloid biosynthesis. The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway. Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi. Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

No MeSH data available.


A) Strict consensus tree of the six most parsimonious trees for dmaW gene products and products of multiple related genes of 34 OTUs based on relatively conserved gene regions. Of the 155 characters, 133 were parsimony-informative; length = 1366, CI = 0.594, RI = 0.499. B) One of the two most parsimonious trees of 32 OTUs based on the whole gene region. Of the 933 characters, 456 were parsimony-informative characters. Length = 4974, CI = 0.682, RI = 0.499. C) The most parsimonious trees for dmaW gene products and products of multiple related genes of 32 OTUs based on relatively conserved gene regions, which were screened by Gblocks. Total aligned characters = 155, informative characters = 135; length = 1427, CI = 0.607, RI = 0.497. OTUs in bold indicate the gene products with functions that have been confirmed. Numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis; numbers in circles indicate the possible gene duplication events (also see Fig. 3).
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f2-ebo-2009-015: A) Strict consensus tree of the six most parsimonious trees for dmaW gene products and products of multiple related genes of 34 OTUs based on relatively conserved gene regions. Of the 155 characters, 133 were parsimony-informative; length = 1366, CI = 0.594, RI = 0.499. B) One of the two most parsimonious trees of 32 OTUs based on the whole gene region. Of the 933 characters, 456 were parsimony-informative characters. Length = 4974, CI = 0.682, RI = 0.499. C) The most parsimonious trees for dmaW gene products and products of multiple related genes of 32 OTUs based on relatively conserved gene regions, which were screened by Gblocks. Total aligned characters = 155, informative characters = 135; length = 1427, CI = 0.607, RI = 0.497. OTUs in bold indicate the gene products with functions that have been confirmed. Numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis; numbers in circles indicate the possible gene duplication events (also see Fig. 3).

Mentions: The analysis with conserved gene regions comprised of 34 OTUs resulted in six equally most parsimonious trees, while the whole gene region (933 characters) resulted in eight equally most parsimonious trees. The variations of branch orders among these trees were mainly from the uncertain positions of the two OTUs, the putative DMATrp synthase gene from Neotyphodium gansuense and paxD Penicillium paxilli AAK11526, which were partial sequences. The strict consensus trees of the six trees from conserved regions and eight trees from the whole gene regions showed the same pattern that, in the dmaW clade, the two putative DMATrp synthase genes from clavicipitaceous endophyte of convolvulaceous plants (AAZ29613, AAZ29614), the clade comprised of C. purpurea and C. fusiformis dmaW, as well as the putative DMATrp synthase gene from N. gansuense collapsed as a polytomy (Fig. 2A). Outside the dmaW clade, paxD P. paxilli AAK11526 along with the other six OTUs appeared as unresolved branches (Fig. 2A). All trees revealed the same monophyletic group of DMATrp synthase genes (functionally tested) and putative DMATrp synthase genes.


Phylogenetic analyses reveal monophyletic origin of the ergot alkaloid gene dmaW in fungi.

Liu M, Panaccione DG, Schardl CL - Evol. Bioinform. Online (2009)

A) Strict consensus tree of the six most parsimonious trees for dmaW gene products and products of multiple related genes of 34 OTUs based on relatively conserved gene regions. Of the 155 characters, 133 were parsimony-informative; length = 1366, CI = 0.594, RI = 0.499. B) One of the two most parsimonious trees of 32 OTUs based on the whole gene region. Of the 933 characters, 456 were parsimony-informative characters. Length = 4974, CI = 0.682, RI = 0.499. C) The most parsimonious trees for dmaW gene products and products of multiple related genes of 32 OTUs based on relatively conserved gene regions, which were screened by Gblocks. Total aligned characters = 155, informative characters = 135; length = 1427, CI = 0.607, RI = 0.497. OTUs in bold indicate the gene products with functions that have been confirmed. Numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis; numbers in circles indicate the possible gene duplication events (also see Fig. 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2747131&req=5

f2-ebo-2009-015: A) Strict consensus tree of the six most parsimonious trees for dmaW gene products and products of multiple related genes of 34 OTUs based on relatively conserved gene regions. Of the 155 characters, 133 were parsimony-informative; length = 1366, CI = 0.594, RI = 0.499. B) One of the two most parsimonious trees of 32 OTUs based on the whole gene region. Of the 933 characters, 456 were parsimony-informative characters. Length = 4974, CI = 0.682, RI = 0.499. C) The most parsimonious trees for dmaW gene products and products of multiple related genes of 32 OTUs based on relatively conserved gene regions, which were screened by Gblocks. Total aligned characters = 155, informative characters = 135; length = 1427, CI = 0.607, RI = 0.497. OTUs in bold indicate the gene products with functions that have been confirmed. Numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis; numbers in circles indicate the possible gene duplication events (also see Fig. 3).
Mentions: The analysis with conserved gene regions comprised of 34 OTUs resulted in six equally most parsimonious trees, while the whole gene region (933 characters) resulted in eight equally most parsimonious trees. The variations of branch orders among these trees were mainly from the uncertain positions of the two OTUs, the putative DMATrp synthase gene from Neotyphodium gansuense and paxD Penicillium paxilli AAK11526, which were partial sequences. The strict consensus trees of the six trees from conserved regions and eight trees from the whole gene regions showed the same pattern that, in the dmaW clade, the two putative DMATrp synthase genes from clavicipitaceous endophyte of convolvulaceous plants (AAZ29613, AAZ29614), the clade comprised of C. purpurea and C. fusiformis dmaW, as well as the putative DMATrp synthase gene from N. gansuense collapsed as a polytomy (Fig. 2A). Outside the dmaW clade, paxD P. paxilli AAK11526 along with the other six OTUs appeared as unresolved branches (Fig. 2A). All trees revealed the same monophyletic group of DMATrp synthase genes (functionally tested) and putative DMATrp synthase genes.

Bottom Line: The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway.Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi.Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

View Article: PubMed Central - PubMed

Affiliation: 201 F Plant Science Bldg, University of Kentucky, Lexington, KY 40546, USA.

ABSTRACT
Ergot alkaloids are indole-derived mycotoxins that are important in agriculture and medicine. Ergot alkaloids are produced by a few representatives of two distantly related fungal lineages, the Clavicipitaceae and the Trichocomaceae. Comparison of the ergot alkaloid gene clusters from these two lineages revealed differences in the relative positions and orientations of several genes. The question arose: is ergot alkaloid biosynthetic capability from a common origin? We used a molecular phylogenetic approach to gain insights into the evolution of ergot alkaloid biosynthesis. The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway. Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi. Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

No MeSH data available.