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Phylogenetic analyses reveal monophyletic origin of the ergot alkaloid gene dmaW in fungi.

Liu M, Panaccione DG, Schardl CL - Evol. Bioinform. Online (2009)

Bottom Line: The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway.Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi.Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

View Article: PubMed Central - PubMed

Affiliation: 201 F Plant Science Bldg, University of Kentucky, Lexington, KY 40546, USA.

ABSTRACT
Ergot alkaloids are indole-derived mycotoxins that are important in agriculture and medicine. Ergot alkaloids are produced by a few representatives of two distantly related fungal lineages, the Clavicipitaceae and the Trichocomaceae. Comparison of the ergot alkaloid gene clusters from these two lineages revealed differences in the relative positions and orientations of several genes. The question arose: is ergot alkaloid biosynthetic capability from a common origin? We used a molecular phylogenetic approach to gain insights into the evolution of ergot alkaloid biosynthesis. The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway. Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi. Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

No MeSH data available.


The most parsimonious trees relating amino acid sequences of the deduced products of six dmaW genes and four related genes with known functions. A) Tree based on whole gene region. Of 653 aligned characters, 250 characters are informative. Length = 1521, CI = 0.870, RI = 0.503. Arrows show the discrepancies of the two most parsimonious trees. B and C) Trees based on relatively conserved gene regions screened by GBlocks. Of 308 total aligned characters, 166 characters are informative. Length = 934, CI = 0.864, RI = 0.512. Products of dmaW genes formed a monophyletic group with 100% bootstrap support. B. Unrooted tree; C. Phylogram rooted by choosing the non-dmaW gene products as the outgroup. numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis. The thick branch separates of the outgroup from the ingroup.
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f1-ebo-2009-015: The most parsimonious trees relating amino acid sequences of the deduced products of six dmaW genes and four related genes with known functions. A) Tree based on whole gene region. Of 653 aligned characters, 250 characters are informative. Length = 1521, CI = 0.870, RI = 0.503. Arrows show the discrepancies of the two most parsimonious trees. B and C) Trees based on relatively conserved gene regions screened by GBlocks. Of 308 total aligned characters, 166 characters are informative. Length = 934, CI = 0.864, RI = 0.512. Products of dmaW genes formed a monophyletic group with 100% bootstrap support. B. Unrooted tree; C. Phylogram rooted by choosing the non-dmaW gene products as the outgroup. numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis. The thick branch separates of the outgroup from the ingroup.

Mentions: For the data set comprised of ten OTUs, parsimony analyses for the whole regions resulted in two most parsimonious trees (Fig. 1A). The two tree topologies differed in the order of branches ranches to sirD L. maculans and fgaPT1 A. fumigatus. The analysis of the conserved regions resulted in one most parsimonious tree. The tree topology differed from those of whole gene regions in the order of divergence of dmaW of A. fumigatus and Malbranchea aurantiaca (Fig. 1B). The known, authentic sequences of DMATrp synthases formed a monophyletic clade with strong bootstrap support. Defining as an outgroup those prenyl transferases known or likely to catalyze production of other products (4 OTUs), the most basal divergence separated the dmaW gene of M. aurantiaca and A. fumigatus from those of the Clavicipitaceae (Fig. 1C).


Phylogenetic analyses reveal monophyletic origin of the ergot alkaloid gene dmaW in fungi.

Liu M, Panaccione DG, Schardl CL - Evol. Bioinform. Online (2009)

The most parsimonious trees relating amino acid sequences of the deduced products of six dmaW genes and four related genes with known functions. A) Tree based on whole gene region. Of 653 aligned characters, 250 characters are informative. Length = 1521, CI = 0.870, RI = 0.503. Arrows show the discrepancies of the two most parsimonious trees. B and C) Trees based on relatively conserved gene regions screened by GBlocks. Of 308 total aligned characters, 166 characters are informative. Length = 934, CI = 0.864, RI = 0.512. Products of dmaW genes formed a monophyletic group with 100% bootstrap support. B. Unrooted tree; C. Phylogram rooted by choosing the non-dmaW gene products as the outgroup. numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis. The thick branch separates of the outgroup from the ingroup.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2747131&req=5

f1-ebo-2009-015: The most parsimonious trees relating amino acid sequences of the deduced products of six dmaW genes and four related genes with known functions. A) Tree based on whole gene region. Of 653 aligned characters, 250 characters are informative. Length = 1521, CI = 0.870, RI = 0.503. Arrows show the discrepancies of the two most parsimonious trees. B and C) Trees based on relatively conserved gene regions screened by GBlocks. Of 308 total aligned characters, 166 characters are informative. Length = 934, CI = 0.864, RI = 0.512. Products of dmaW genes formed a monophyletic group with 100% bootstrap support. B. Unrooted tree; C. Phylogram rooted by choosing the non-dmaW gene products as the outgroup. numbers on branches indicate bootstrap percentage of MP/posterior probabilities of BI/bootstrap percentage of ML; *indicates that particular branch does not exist in the analysis. The thick branch separates of the outgroup from the ingroup.
Mentions: For the data set comprised of ten OTUs, parsimony analyses for the whole regions resulted in two most parsimonious trees (Fig. 1A). The two tree topologies differed in the order of branches ranches to sirD L. maculans and fgaPT1 A. fumigatus. The analysis of the conserved regions resulted in one most parsimonious tree. The tree topology differed from those of whole gene regions in the order of divergence of dmaW of A. fumigatus and Malbranchea aurantiaca (Fig. 1B). The known, authentic sequences of DMATrp synthases formed a monophyletic clade with strong bootstrap support. Defining as an outgroup those prenyl transferases known or likely to catalyze production of other products (4 OTUs), the most basal divergence separated the dmaW gene of M. aurantiaca and A. fumigatus from those of the Clavicipitaceae (Fig. 1C).

Bottom Line: The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway.Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi.Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

View Article: PubMed Central - PubMed

Affiliation: 201 F Plant Science Bldg, University of Kentucky, Lexington, KY 40546, USA.

ABSTRACT
Ergot alkaloids are indole-derived mycotoxins that are important in agriculture and medicine. Ergot alkaloids are produced by a few representatives of two distantly related fungal lineages, the Clavicipitaceae and the Trichocomaceae. Comparison of the ergot alkaloid gene clusters from these two lineages revealed differences in the relative positions and orientations of several genes. The question arose: is ergot alkaloid biosynthetic capability from a common origin? We used a molecular phylogenetic approach to gain insights into the evolution of ergot alkaloid biosynthesis. The 4-gamma,gamma-dimethylallyltryptophan synthase gene, dmaW, encodes the first step in the pathway. Amino acid sequences deduced from dmaW and homologs were submitted to phylogenetic analysis, and the results indicated that dmaW of Aspergillus fumigatus (mitosporic Trichocomaceae) has the same origin as corresponding genes from clavicipitaceous fungi. Relationships of authentic dmaW genes suggest that they originated from multiple gene duplications with subsequent losses of original or duplicate versions in some lineages.

No MeSH data available.