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Synergistic antibacterial and anti-inflammatory activity of temporin A and modified temporin B in vivo.

Capparelli R, Romanelli A, Iannaccone M, Nocerino N, Ripa R, Pensato S, Pedone C, Iannelli D - PLoS ONE (2009)

Bottom Line: Temporins are antimicrobial peptides secreted by the granular glands of the European red frog (Rana temporaria).Administration of 3.4 mg/Kg of temporin A (TA)+1.6 mg/Kg TB-YK, given to individual mice concurrently with a lethal dose of bacteria (gram positive or negative), rescued 100% of the animals.The model offers the possibility to test in a preclinical setting the true potential of TA and TB-YK in combination as antimicrobial and anti-inflammatory agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil, Plant, Environment and Animal Production Sciences, University of Naples Federico II, School of Biotechnological Sciences, Napoli, Italy. capparel@unina.it

ABSTRACT
Temporins are antimicrobial peptides secreted by the granular glands of the European red frog (Rana temporaria). They are 10-14 amino acid long polypeptides active prevalently against gram positive bacteria. This study shows that a synthetic temporin B analogue (TB-YK), acquires the capacity to act in synergism with temporin A and to exert antimicrobial and anti-inflammatory activity in vivo against gram positive and gram negative bacteria. Administration of 3.4 mg/Kg of temporin A (TA)+1.6 mg/Kg TB-YK, given to individual mice concurrently with a lethal dose of bacteria (gram positive or negative), rescued 100% of the animals. More importantly, the same doses of temporins, administered one week after experimental infection with a sub lethal dose of bacteria, sterilized 100% of the animals within 3-6 days. Also, it is described an animal model based on the use of sub lethal doses of bacteria, which closely mimics bacterial infection in humans. The model offers the possibility to test in a preclinical setting the true potential of TA and TB-YK in combination as antimicrobial and anti-inflammatory agents.

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Mice infected with GFP-labelled bacteria were analysed using the Leica macrofluo instrument (Wetzlar, Germany) equipped with the Leica application suite 3.1.0 software.A: liver from mice infected with S. enterica serovar Paratyphi B; B: liver from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB-YK; C: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B; D: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB.
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pone-0007191-g010: Mice infected with GFP-labelled bacteria were analysed using the Leica macrofluo instrument (Wetzlar, Germany) equipped with the Leica application suite 3.1.0 software.A: liver from mice infected with S. enterica serovar Paratyphi B; B: liver from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB-YK; C: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B; D: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB.

Mentions: Mice were infected with a sub-lethal dose of Salmonella GFP 106 CFU/mouse and immediately treated with the 35/75 pool. 24 hours later, the animals were sacrificed and intestine and liver were extracted. Observation of the organs at the Macrofluo microscope revealed a reduction in the fluorescence of the treated mice, as compared to untreated mice, which further confirms the efficacy of the pool 35/75. (Figure 10)


Synergistic antibacterial and anti-inflammatory activity of temporin A and modified temporin B in vivo.

Capparelli R, Romanelli A, Iannaccone M, Nocerino N, Ripa R, Pensato S, Pedone C, Iannelli D - PLoS ONE (2009)

Mice infected with GFP-labelled bacteria were analysed using the Leica macrofluo instrument (Wetzlar, Germany) equipped with the Leica application suite 3.1.0 software.A: liver from mice infected with S. enterica serovar Paratyphi B; B: liver from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB-YK; C: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B; D: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747021&req=5

pone-0007191-g010: Mice infected with GFP-labelled bacteria were analysed using the Leica macrofluo instrument (Wetzlar, Germany) equipped with the Leica application suite 3.1.0 software.A: liver from mice infected with S. enterica serovar Paratyphi B; B: liver from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB-YK; C: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B; D: gastro instestinal tract from mice infected with S. enterica serovar Paratyphi B and immediately treated with TA plus TB.
Mentions: Mice were infected with a sub-lethal dose of Salmonella GFP 106 CFU/mouse and immediately treated with the 35/75 pool. 24 hours later, the animals were sacrificed and intestine and liver were extracted. Observation of the organs at the Macrofluo microscope revealed a reduction in the fluorescence of the treated mice, as compared to untreated mice, which further confirms the efficacy of the pool 35/75. (Figure 10)

Bottom Line: Temporins are antimicrobial peptides secreted by the granular glands of the European red frog (Rana temporaria).Administration of 3.4 mg/Kg of temporin A (TA)+1.6 mg/Kg TB-YK, given to individual mice concurrently with a lethal dose of bacteria (gram positive or negative), rescued 100% of the animals.The model offers the possibility to test in a preclinical setting the true potential of TA and TB-YK in combination as antimicrobial and anti-inflammatory agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil, Plant, Environment and Animal Production Sciences, University of Naples Federico II, School of Biotechnological Sciences, Napoli, Italy. capparel@unina.it

ABSTRACT
Temporins are antimicrobial peptides secreted by the granular glands of the European red frog (Rana temporaria). They are 10-14 amino acid long polypeptides active prevalently against gram positive bacteria. This study shows that a synthetic temporin B analogue (TB-YK), acquires the capacity to act in synergism with temporin A and to exert antimicrobial and anti-inflammatory activity in vivo against gram positive and gram negative bacteria. Administration of 3.4 mg/Kg of temporin A (TA)+1.6 mg/Kg TB-YK, given to individual mice concurrently with a lethal dose of bacteria (gram positive or negative), rescued 100% of the animals. More importantly, the same doses of temporins, administered one week after experimental infection with a sub lethal dose of bacteria, sterilized 100% of the animals within 3-6 days. Also, it is described an animal model based on the use of sub lethal doses of bacteria, which closely mimics bacterial infection in humans. The model offers the possibility to test in a preclinical setting the true potential of TA and TB-YK in combination as antimicrobial and anti-inflammatory agents.

Show MeSH
Related in: MedlinePlus