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Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.

Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS, Chen SY - PLoS ONE (2009)

Bottom Line: Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants.This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

View Article: PubMed Central - PubMed

Affiliation: Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT

Background: Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.

Principal findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element "GTGGAG". The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.

Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

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Related in: MedlinePlus

Performance of the GmPHD2-transgenic plants under salt stress.(A) Seed germination under salt stress. The seed germination rate of transgenic lines (G2–3, G2–6, G2–8) was calculated 5 d after sowing. Each data point is the means of three replicates and bars indicate SD. (B) Plant growth in NaCl medium. Five-day-old seedlings were treated on plate without (CK, top) or with 150 mM NaCl (NaCl treated, bottom) for two weeks. (C) Recovery of salt-stressed plants in pots. Seedlings treated with 150 mM NaCl (NaCl treated, bottom) or without NaCl (CK, top) were transferred to pots and grown for two weeks under normal conditions. (D) Comparison of plant height after salt stress treatment. Plant heights in (C) were measured. Values are means±SD (n = 54). (E) Comparison of root length after salt stress treatment. Root length of plants in (C) was measured. Values are means±SD (n = 54). For (A), (D) and (E), “*” and “**” indicate significant difference (P<0.05 and P<0.01 respectively) compared to the corresponding WT plants.
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pone-0007209-g008: Performance of the GmPHD2-transgenic plants under salt stress.(A) Seed germination under salt stress. The seed germination rate of transgenic lines (G2–3, G2–6, G2–8) was calculated 5 d after sowing. Each data point is the means of three replicates and bars indicate SD. (B) Plant growth in NaCl medium. Five-day-old seedlings were treated on plate without (CK, top) or with 150 mM NaCl (NaCl treated, bottom) for two weeks. (C) Recovery of salt-stressed plants in pots. Seedlings treated with 150 mM NaCl (NaCl treated, bottom) or without NaCl (CK, top) were transferred to pots and grown for two weeks under normal conditions. (D) Comparison of plant height after salt stress treatment. Plant heights in (C) were measured. Values are means±SD (n = 54). (E) Comparison of root length after salt stress treatment. Root length of plants in (C) was measured. Values are means±SD (n = 54). For (A), (D) and (E), “*” and “**” indicate significant difference (P<0.05 and P<0.01 respectively) compared to the corresponding WT plants.

Mentions: Because the GmPHD genes were responsive to multiple stresses, we investigated if the GmPHDs are involved in stress responses. The GmPHD2 was used for further analysis because the encoded protein showed the least homology to the well-studied Alfin1 [10]. We generated the transgenic Arabidopsis plants overexpressing the GmPHD2 gene under the control of 35S promoter. Three homozygous lines G2–3, G2–6 and G2–8, with higher GmPHD2 expression (Fig. 8, 9), were analyzed for their performance under salt stress condition. Fig. 8A showed that under normal condition, the germination rate of GmPHD2-trangenic seeds was similar to that of the wild type plants. Under NaCl treatment, the germination rate of the transgenic plants was significantly higher than that in the wild type plants. These results indicate that overexpression of GmPHD2 in Arabidopsis enhanced the salt tolerance of the transgenic plants at germination stage.


Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.

Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS, Chen SY - PLoS ONE (2009)

Performance of the GmPHD2-transgenic plants under salt stress.(A) Seed germination under salt stress. The seed germination rate of transgenic lines (G2–3, G2–6, G2–8) was calculated 5 d after sowing. Each data point is the means of three replicates and bars indicate SD. (B) Plant growth in NaCl medium. Five-day-old seedlings were treated on plate without (CK, top) or with 150 mM NaCl (NaCl treated, bottom) for two weeks. (C) Recovery of salt-stressed plants in pots. Seedlings treated with 150 mM NaCl (NaCl treated, bottom) or without NaCl (CK, top) were transferred to pots and grown for two weeks under normal conditions. (D) Comparison of plant height after salt stress treatment. Plant heights in (C) were measured. Values are means±SD (n = 54). (E) Comparison of root length after salt stress treatment. Root length of plants in (C) was measured. Values are means±SD (n = 54). For (A), (D) and (E), “*” and “**” indicate significant difference (P<0.05 and P<0.01 respectively) compared to the corresponding WT plants.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747011&req=5

pone-0007209-g008: Performance of the GmPHD2-transgenic plants under salt stress.(A) Seed germination under salt stress. The seed germination rate of transgenic lines (G2–3, G2–6, G2–8) was calculated 5 d after sowing. Each data point is the means of three replicates and bars indicate SD. (B) Plant growth in NaCl medium. Five-day-old seedlings were treated on plate without (CK, top) or with 150 mM NaCl (NaCl treated, bottom) for two weeks. (C) Recovery of salt-stressed plants in pots. Seedlings treated with 150 mM NaCl (NaCl treated, bottom) or without NaCl (CK, top) were transferred to pots and grown for two weeks under normal conditions. (D) Comparison of plant height after salt stress treatment. Plant heights in (C) were measured. Values are means±SD (n = 54). (E) Comparison of root length after salt stress treatment. Root length of plants in (C) was measured. Values are means±SD (n = 54). For (A), (D) and (E), “*” and “**” indicate significant difference (P<0.05 and P<0.01 respectively) compared to the corresponding WT plants.
Mentions: Because the GmPHD genes were responsive to multiple stresses, we investigated if the GmPHDs are involved in stress responses. The GmPHD2 was used for further analysis because the encoded protein showed the least homology to the well-studied Alfin1 [10]. We generated the transgenic Arabidopsis plants overexpressing the GmPHD2 gene under the control of 35S promoter. Three homozygous lines G2–3, G2–6 and G2–8, with higher GmPHD2 expression (Fig. 8, 9), were analyzed for their performance under salt stress condition. Fig. 8A showed that under normal condition, the germination rate of GmPHD2-trangenic seeds was similar to that of the wild type plants. Under NaCl treatment, the germination rate of the transgenic plants was significantly higher than that in the wild type plants. These results indicate that overexpression of GmPHD2 in Arabidopsis enhanced the salt tolerance of the transgenic plants at germination stage.

Bottom Line: Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants.This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

View Article: PubMed Central - PubMed

Affiliation: Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT

Background: Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.

Principal findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element "GTGGAG". The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.

Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

Show MeSH
Related in: MedlinePlus