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Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.

Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS, Chen SY - PLoS ONE (2009)

Bottom Line: Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants.This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

View Article: PubMed Central - PubMed

Affiliation: Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT

Background: Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.

Principal findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element "GTGGAG". The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.

Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

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Related in: MedlinePlus

Expression of the six GmPHD genes in stress-tolerant cultivar JD23 and stress-sensitive cultivar HBZ under various treatments.Two-week-old soybean seedlings were subjected to treatments with 200 mM NaCl, 100 µM ABA, cold and drought, and total RNA was isolated for real-time quantitative PCR analysis.
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pone-0007209-g003: Expression of the six GmPHD genes in stress-tolerant cultivar JD23 and stress-sensitive cultivar HBZ under various treatments.Two-week-old soybean seedlings were subjected to treatments with 200 mM NaCl, 100 µM ABA, cold and drought, and total RNA was isolated for real-time quantitative PCR analysis.

Mentions: Expressions of the six GmPHDs were investigated in JD23 and HBZ in response to different treatments, including high NaCl, drought, ABA and cold (Fig. 3). All of the genes were induced in response to drought, but showed differences in responses to the other stresses. One of the genes, GmPHD4, was induced in response to all four conditions while the other five genes were induced in response to two or three conditions. Interestingly, the GmPHD4 and GmPHD5 were the only genes induced in response to low temperature and in both cases, this was only observed in the more stress-tolerant line JD23. These results indicate that the six GmPHD genes were differentially regulated in response to various treatments, and in most cases, the inductions of the GmPHD genes were stronger in stress-tolerant JD23 than those in stress-sensitive HBZ.


Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.

Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS, Chen SY - PLoS ONE (2009)

Expression of the six GmPHD genes in stress-tolerant cultivar JD23 and stress-sensitive cultivar HBZ under various treatments.Two-week-old soybean seedlings were subjected to treatments with 200 mM NaCl, 100 µM ABA, cold and drought, and total RNA was isolated for real-time quantitative PCR analysis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747011&req=5

pone-0007209-g003: Expression of the six GmPHD genes in stress-tolerant cultivar JD23 and stress-sensitive cultivar HBZ under various treatments.Two-week-old soybean seedlings were subjected to treatments with 200 mM NaCl, 100 µM ABA, cold and drought, and total RNA was isolated for real-time quantitative PCR analysis.
Mentions: Expressions of the six GmPHDs were investigated in JD23 and HBZ in response to different treatments, including high NaCl, drought, ABA and cold (Fig. 3). All of the genes were induced in response to drought, but showed differences in responses to the other stresses. One of the genes, GmPHD4, was induced in response to all four conditions while the other five genes were induced in response to two or three conditions. Interestingly, the GmPHD4 and GmPHD5 were the only genes induced in response to low temperature and in both cases, this was only observed in the more stress-tolerant line JD23. These results indicate that the six GmPHD genes were differentially regulated in response to various treatments, and in most cases, the inductions of the GmPHD genes were stronger in stress-tolerant JD23 than those in stress-sensitive HBZ.

Bottom Line: Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants.This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

View Article: PubMed Central - PubMed

Affiliation: Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT

Background: Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.

Principal findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element "GTGGAG". The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.

Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

Show MeSH
Related in: MedlinePlus