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Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.

Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS, Chen SY - PLoS ONE (2009)

Bottom Line: Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants.This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

View Article: PubMed Central - PubMed

Affiliation: Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT

Background: Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.

Principal findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element "GTGGAG". The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.

Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

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Related in: MedlinePlus

Cluster analysis of the PHD finger proteins from different plants.The analysis was performed by using the MEGA 4.0 program with neighbor joining method and with 1000 replicates. Numbers on the figure are bootstrap values. The sequences are from soybean (GmPHDs), Medicago (MtPHDs), Arabidopsis and rice plants.
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pone-0007209-g002: Cluster analysis of the PHD finger proteins from different plants.The analysis was performed by using the MEGA 4.0 program with neighbor joining method and with 1000 replicates. Numbers on the figure are bootstrap values. The sequences are from soybean (GmPHDs), Medicago (MtPHDs), Arabidopsis and rice plants.

Mentions: The soybean GmPHDs showed 67% to 89% sequence identity to alfalfa Alfin1 (L07291) [10]. Homologues of GmPHDs were also present in many other plant species such as Arabidopsis, rice, Medicago and Solanum tuberosum. In Medicago truncatula, seven homologues were found and five were full-length sequence termed MtPHD1 to 5 (EF025125, EF025126, EF025127, EF025128, and EF025129). MtPHD5 was almost identical to the Alfin1 [10]. From Arabidopsis and rice databases, seven homologues were also found respectively, including AT1G14510, AT2G02470, AT3G11200, AT3G42790, AT5G05610, AT5G20510, AT5G26210, Os04g0444900, Os05g0163100, Os07g0233300, Os03g0818300, Os05g0419100, Os01g0887700, and Os07g0608400. GmPHDs has an overall identity of 68% to 72% compared to these homologues. The cluster analysis revealed that the GmPHD2, GmPHD4 and GmPHD6 were more closely related whereas GmPHD1 and GmPHD3 grouped with MtPHD1 and MtPHD3 respectively (Fig. 2). The GmPHD5 was clustered with MtPHD5 and may be more divergent when compared with the other GmPHD proteins (Fig. 2).


Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.

Wei W, Huang J, Hao YJ, Zou HF, Wang HW, Zhao JY, Liu XY, Zhang WK, Ma B, Zhang JS, Chen SY - PLoS ONE (2009)

Cluster analysis of the PHD finger proteins from different plants.The analysis was performed by using the MEGA 4.0 program with neighbor joining method and with 1000 replicates. Numbers on the figure are bootstrap values. The sequences are from soybean (GmPHDs), Medicago (MtPHDs), Arabidopsis and rice plants.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2747011&req=5

pone-0007209-g002: Cluster analysis of the PHD finger proteins from different plants.The analysis was performed by using the MEGA 4.0 program with neighbor joining method and with 1000 replicates. Numbers on the figure are bootstrap values. The sequences are from soybean (GmPHDs), Medicago (MtPHDs), Arabidopsis and rice plants.
Mentions: The soybean GmPHDs showed 67% to 89% sequence identity to alfalfa Alfin1 (L07291) [10]. Homologues of GmPHDs were also present in many other plant species such as Arabidopsis, rice, Medicago and Solanum tuberosum. In Medicago truncatula, seven homologues were found and five were full-length sequence termed MtPHD1 to 5 (EF025125, EF025126, EF025127, EF025128, and EF025129). MtPHD5 was almost identical to the Alfin1 [10]. From Arabidopsis and rice databases, seven homologues were also found respectively, including AT1G14510, AT2G02470, AT3G11200, AT3G42790, AT5G05610, AT5G20510, AT5G26210, Os04g0444900, Os05g0163100, Os07g0233300, Os03g0818300, Os05g0419100, Os01g0887700, and Os07g0608400. GmPHDs has an overall identity of 68% to 72% compared to these homologues. The cluster analysis revealed that the GmPHD2, GmPHD4 and GmPHD6 were more closely related whereas GmPHD1 and GmPHD3 grouped with MtPHD1 and MtPHD3 respectively (Fig. 2). The GmPHD5 was clustered with MtPHD5 and may be more divergent when compared with the other GmPHD proteins (Fig. 2).

Bottom Line: Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants.This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

View Article: PubMed Central - PubMed

Affiliation: Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

ABSTRACT

Background: Soybean [Glycine max (L.) Merr.] is one of the most important crops for oil and protein resource. Improvement of stress tolerance will be beneficial for soybean seed production.

Principal findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed ability to bind the cis-element "GTGGAG". The N-terminal domain of GmPHD played a major role in DNA binding. Using a protoplast assay system, we find that GmPHD1 to GmPHD5 had transcriptional suppression activity whereas GmPHD6 did not have. In yeast assay, the GmPHD6 can form homodimer and heterodimer with the other GmPHDs except GmPHD2. The N-terminal plus the variable regions but not the PHD-finger is required for the dimerization. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achieved by diminishing the oxidative stress through regulation of downstream genes.

Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcription regulator.

Show MeSH
Related in: MedlinePlus