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Dietary restriction reduces angiogenesis and growth in an orthotopic mouse brain tumour model.

Mukherjee P, El-Abbadi MM, Kasperzyk JL, Ranes MK, Seyfried TN - Br. J. Cancer (2002)

Bottom Line: We found that a moderate dietary restriction of 30-40% significantly inhibited the intracerebral growth of the CT-2A syngeneic malignant mouse astrocytoma by almost 80%.These findings indicate that dietary restriction inhibited CT-2A growth by reducing angiogenesis and by enhancing apoptosis.Our data suggest that moderate dietary restriction may be an effective antiangiogenic therapy for recurrent malignant brain cancers.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Boston College, Chestnut Hill, Massachusetts, MA 02467, USA.

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Influence of DR on microvessel density and apoptosis in the CT-2A brain tumour. DR was initiated 7 days before intracerebral tumour implantation and was continued for 11 days. H&E stained tumour sections in an AL mouse (A) and in a DR mouse (B) (100×). Factor VIII immunostaining from the tumour grown in an AL mouse (C) and in a DR mouse (D) (200×). TUNEL positive apoptotic cells (arrows) from the tumour grown in an AL mouse (E) and in a DR mouse (F) (400×). Each stained section was representative of the entire tumour. All images were produced from digital photography.
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fig3: Influence of DR on microvessel density and apoptosis in the CT-2A brain tumour. DR was initiated 7 days before intracerebral tumour implantation and was continued for 11 days. H&E stained tumour sections in an AL mouse (A) and in a DR mouse (B) (100×). Factor VIII immunostaining from the tumour grown in an AL mouse (C) and in a DR mouse (D) (200×). TUNEL positive apoptotic cells (arrows) from the tumour grown in an AL mouse (E) and in a DR mouse (F) (400×). Each stained section was representative of the entire tumour. All images were produced from digital photography.

Mentions: We next examined tumour morphology and blood vessel densities using H&E staining and Factor VIII immunostaining to determine if DR influenced tumour angiogenesis. Three independent tumours from the AL and DR groups were chosen at random for these studies. The number and size of blood vessels and tumour cell density were noticeably less in the DR-fed mice than in the AL-fed mice (Figure 3A,BFigure 3


Dietary restriction reduces angiogenesis and growth in an orthotopic mouse brain tumour model.

Mukherjee P, El-Abbadi MM, Kasperzyk JL, Ranes MK, Seyfried TN - Br. J. Cancer (2002)

Influence of DR on microvessel density and apoptosis in the CT-2A brain tumour. DR was initiated 7 days before intracerebral tumour implantation and was continued for 11 days. H&E stained tumour sections in an AL mouse (A) and in a DR mouse (B) (100×). Factor VIII immunostaining from the tumour grown in an AL mouse (C) and in a DR mouse (D) (200×). TUNEL positive apoptotic cells (arrows) from the tumour grown in an AL mouse (E) and in a DR mouse (F) (400×). Each stained section was representative of the entire tumour. All images were produced from digital photography.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746602&req=5

fig3: Influence of DR on microvessel density and apoptosis in the CT-2A brain tumour. DR was initiated 7 days before intracerebral tumour implantation and was continued for 11 days. H&E stained tumour sections in an AL mouse (A) and in a DR mouse (B) (100×). Factor VIII immunostaining from the tumour grown in an AL mouse (C) and in a DR mouse (D) (200×). TUNEL positive apoptotic cells (arrows) from the tumour grown in an AL mouse (E) and in a DR mouse (F) (400×). Each stained section was representative of the entire tumour. All images were produced from digital photography.
Mentions: We next examined tumour morphology and blood vessel densities using H&E staining and Factor VIII immunostaining to determine if DR influenced tumour angiogenesis. Three independent tumours from the AL and DR groups were chosen at random for these studies. The number and size of blood vessels and tumour cell density were noticeably less in the DR-fed mice than in the AL-fed mice (Figure 3A,BFigure 3

Bottom Line: We found that a moderate dietary restriction of 30-40% significantly inhibited the intracerebral growth of the CT-2A syngeneic malignant mouse astrocytoma by almost 80%.These findings indicate that dietary restriction inhibited CT-2A growth by reducing angiogenesis and by enhancing apoptosis.Our data suggest that moderate dietary restriction may be an effective antiangiogenic therapy for recurrent malignant brain cancers.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Boston College, Chestnut Hill, Massachusetts, MA 02467, USA.

Show MeSH
Related in: MedlinePlus