Orthogonal polarisation spectral imaging as a new tool for the assessment of antivascular tumour treatment in vivo: a validation study.
Bottom Line: Correlation parameters for diameter of microvessels and red blood cell velocity were similar (r2=0.97, R(S)=0.99 and r2=0.93, R(S)=0.94 for diameter of microvessels and red blood cell velocity, respectively).Treatment with SU5416 reduced tumour angiogenesis.In addition to the inhibition of tumour angiogenesis, tumour growth and the development of metastasis was strongly reduced in SU5416 treated animals.
Affiliation: Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Marchioninistrasse 15, 81377 Munich, Germany. Pahernik@Urologie.Klinik.Uni-Mainz.deShow MeSH
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Mentions: Orthogonal polarisation spectral™ (OPS) imaging is a novel technique which has been introduced recently to visualise microvessels in vivo where tissue is illuminated with linearly polarised light and imaged through a polariser oriented orthogonal to the plane of the illuminating light (Groner et al, 1999). Using this technique close to wavelengths within the haemoglobin absorption spectrum, microvessels containing red blood cells can be visualised. The OPS imaging system does not require transillumination or fluorescent dyes for contrast enhancement and can be positioned directly on top of the tumour to visualise tumour vessels. The small size of the probe facilitates its use as a non-invasive diagnostic tool not only experimentally but more importantly in clinical settings to evaluate and monitor tumour angiogenesis (Figure 1AFigure 5
Affiliation: Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Marchioninistrasse 15, 81377 Munich, Germany. Pahernik@Urologie.Klinik.Uni-Mainz.de