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Investigations on a clinically and functionally unusual and novel germline p53 mutation.

Rutherford J, Chu CE, Duddy PM, Charlton RS, Chumas P, Taylor GR, Lu X, Barnes DM, Camplejohn RS - Br. J. Cancer (2002)

Bottom Line: Surprisingly two assays of p53 function gave apparently wild-type results on peripheral blood lymphocytes from this individual.These results led us to carry out more detailed functional tests on the mutant protein.However, surprisingly, data from irradiated peripheral blood lymphocytes and transfected Saos-2 cells, suggested that this truncated, mutant protein retains significant ability to induce apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Richard Dimbleby Department Cancer Research, Guy's, King's and St Thomas' School of Medicine, St Thomas' Hospital, London SE1 7EH, UK.

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Induction of apoptosis. The pC53-SN3 plasmid containing the p53 insert of interest was transfected into Saos-2 cells, which were washed after 16 h and reincubated for 72 h. The results show that the 7 base pair mutation retained about 65% of the apoptotic ability of WT p53. 344P, the non-functional mutant only retained about 20% apoptotic ability. Statistical analysis showed that the 7 base pair mutation was significantly different from both 344P and WT, with P<0.02.
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fig5: Induction of apoptosis. The pC53-SN3 plasmid containing the p53 insert of interest was transfected into Saos-2 cells, which were washed after 16 h and reincubated for 72 h. The results show that the 7 base pair mutation retained about 65% of the apoptotic ability of WT p53. 344P, the non-functional mutant only retained about 20% apoptotic ability. Statistical analysis showed that the 7 base pair mutation was significantly different from both 344P and WT, with P<0.02.

Mentions: Apoptosis in Saos-2 cells can be induced by the expression of wild-type p53. Saos-2 cells were transfected with the mammalian expression plasmid pC53-SN3, which contained the 7 base pair insertion mutation of p53. Controls used included wild-type p53, 337C p53 (a semi-functional mutant) and 344P p53. The results showed that the 7 base pair insertion mutation retains 65% of the ability to induce apoptosis compared with wild-type (Figure 5Figure 5


Investigations on a clinically and functionally unusual and novel germline p53 mutation.

Rutherford J, Chu CE, Duddy PM, Charlton RS, Chumas P, Taylor GR, Lu X, Barnes DM, Camplejohn RS - Br. J. Cancer (2002)

Induction of apoptosis. The pC53-SN3 plasmid containing the p53 insert of interest was transfected into Saos-2 cells, which were washed after 16 h and reincubated for 72 h. The results show that the 7 base pair mutation retained about 65% of the apoptotic ability of WT p53. 344P, the non-functional mutant only retained about 20% apoptotic ability. Statistical analysis showed that the 7 base pair mutation was significantly different from both 344P and WT, with P<0.02.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746598&req=5

fig5: Induction of apoptosis. The pC53-SN3 plasmid containing the p53 insert of interest was transfected into Saos-2 cells, which were washed after 16 h and reincubated for 72 h. The results show that the 7 base pair mutation retained about 65% of the apoptotic ability of WT p53. 344P, the non-functional mutant only retained about 20% apoptotic ability. Statistical analysis showed that the 7 base pair mutation was significantly different from both 344P and WT, with P<0.02.
Mentions: Apoptosis in Saos-2 cells can be induced by the expression of wild-type p53. Saos-2 cells were transfected with the mammalian expression plasmid pC53-SN3, which contained the 7 base pair insertion mutation of p53. Controls used included wild-type p53, 337C p53 (a semi-functional mutant) and 344P p53. The results showed that the 7 base pair insertion mutation retains 65% of the ability to induce apoptosis compared with wild-type (Figure 5Figure 5

Bottom Line: Surprisingly two assays of p53 function gave apparently wild-type results on peripheral blood lymphocytes from this individual.These results led us to carry out more detailed functional tests on the mutant protein.However, surprisingly, data from irradiated peripheral blood lymphocytes and transfected Saos-2 cells, suggested that this truncated, mutant protein retains significant ability to induce apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Richard Dimbleby Department Cancer Research, Guy's, King's and St Thomas' School of Medicine, St Thomas' Hospital, London SE1 7EH, UK.

Show MeSH
Related in: MedlinePlus