Investigations on a clinically and functionally unusual and novel germline p53 mutation.
Bottom Line: Surprisingly two assays of p53 function gave apparently wild-type results on peripheral blood lymphocytes from this individual.These results led us to carry out more detailed functional tests on the mutant protein.However, surprisingly, data from irradiated peripheral blood lymphocytes and transfected Saos-2 cells, suggested that this truncated, mutant protein retains significant ability to induce apoptosis.
Affiliation: Richard Dimbleby Department Cancer Research, Guy's, King's and St Thomas' School of Medicine, St Thomas' Hospital, London SE1 7EH, UK.Show MeSH
Related in: MedlinePlus
Mentions: The manufactured 7 base pair insertion mutation was cloned into a mammalian expression plasmid and was transfected into Saos-2 cells. Cell lysates were made of these cells and of Saos-2 cells transfected with wild-type p53. PHA stimulated lymphocytes from the patient were also lysed and a Western blot was run using all three lysates (Figure 4Figure 4
Affiliation: Richard Dimbleby Department Cancer Research, Guy's, King's and St Thomas' School of Medicine, St Thomas' Hospital, London SE1 7EH, UK.