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Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2).

Salucci M, Stivala LA, Maiani G, Bugianesi R, Vannini V - Br. J. Cancer (2002)

Bottom Line: Quercetin, the major flavonoid present in vegetables and fruit, exerts potential anticarcinogenic effects in animal models and cell cultures, but less is known about quercetin glucosides.The results of this study indicate that all dietary flavonoids studied (epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside) show a significant antioxidant effect in a chemical model system, but only epigallocatechin gallate or gallic acid are able to interfere with the cell cycle in Caco2 cell lines.From a structural point of view, the galloyl moiety appears to be required for both the antioxidant and the antiproliferative effects.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Food and Nutrition Research, Via Ardeatina 546, 00178, Rome, Italy. salucci@inran.it

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Frequency distributions of BrdU immunofluorescence vs DNA content in untreated and treated cells. Cells were incubated without and with 100 μM of EGCG, EC, GA and 70 μM of IQ for 24 h, and the percentage of cells in each phase was measured by flow cytometry. Three independent experiments were made.
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fig6: Frequency distributions of BrdU immunofluorescence vs DNA content in untreated and treated cells. Cells were incubated without and with 100 μM of EGCG, EC, GA and 70 μM of IQ for 24 h, and the percentage of cells in each phase was measured by flow cytometry. Three independent experiments were made.

Mentions: EGCG and GA significantly increased the number of cells in G2/M phase (P<0.05), with a consequent reduction of cells in G1 and S phases. In fact, after 24 h of incubation, the number of cells in G2/M phase was increased by about 33 and 40% for EGCG and GA, respectively. On the contrary, responses to EC and IQ were similar to those of control cells. Figure 6Figure 6


Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2).

Salucci M, Stivala LA, Maiani G, Bugianesi R, Vannini V - Br. J. Cancer (2002)

Frequency distributions of BrdU immunofluorescence vs DNA content in untreated and treated cells. Cells were incubated without and with 100 μM of EGCG, EC, GA and 70 μM of IQ for 24 h, and the percentage of cells in each phase was measured by flow cytometry. Three independent experiments were made.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746583&req=5

fig6: Frequency distributions of BrdU immunofluorescence vs DNA content in untreated and treated cells. Cells were incubated without and with 100 μM of EGCG, EC, GA and 70 μM of IQ for 24 h, and the percentage of cells in each phase was measured by flow cytometry. Three independent experiments were made.
Mentions: EGCG and GA significantly increased the number of cells in G2/M phase (P<0.05), with a consequent reduction of cells in G1 and S phases. In fact, after 24 h of incubation, the number of cells in G2/M phase was increased by about 33 and 40% for EGCG and GA, respectively. On the contrary, responses to EC and IQ were similar to those of control cells. Figure 6Figure 6

Bottom Line: Quercetin, the major flavonoid present in vegetables and fruit, exerts potential anticarcinogenic effects in animal models and cell cultures, but less is known about quercetin glucosides.The results of this study indicate that all dietary flavonoids studied (epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside) show a significant antioxidant effect in a chemical model system, but only epigallocatechin gallate or gallic acid are able to interfere with the cell cycle in Caco2 cell lines.From a structural point of view, the galloyl moiety appears to be required for both the antioxidant and the antiproliferative effects.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Food and Nutrition Research, Via Ardeatina 546, 00178, Rome, Italy. salucci@inran.it

Show MeSH
Related in: MedlinePlus