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Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2).

Salucci M, Stivala LA, Maiani G, Bugianesi R, Vannini V - Br. J. Cancer (2002)

Bottom Line: Quercetin, the major flavonoid present in vegetables and fruit, exerts potential anticarcinogenic effects in animal models and cell cultures, but less is known about quercetin glucosides.The results of this study indicate that all dietary flavonoids studied (epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside) show a significant antioxidant effect in a chemical model system, but only epigallocatechin gallate or gallic acid are able to interfere with the cell cycle in Caco2 cell lines.From a structural point of view, the galloyl moiety appears to be required for both the antioxidant and the antiproliferative effects.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Food and Nutrition Research, Via Ardeatina 546, 00178, Rome, Italy. salucci@inran.it

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Time course of cellular uptake of GA on Caco2 cells. Cells were incubated with 100 μM of GA for 10, 30, 60 min and 24 h, and the cellular uptake was evaluated by HPLC. Three independent experiments were made. Bars indicate standard deviation of the mean.
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fig4: Time course of cellular uptake of GA on Caco2 cells. Cells were incubated with 100 μM of GA for 10, 30, 60 min and 24 h, and the cellular uptake was evaluated by HPLC. Three independent experiments were made. Bars indicate standard deviation of the mean.

Mentions: GA was not detectable, both in the medium and in the cells, after 24, 48 and 72 h of incubation. This result was perplexing as GA exerts a clear effect on cell cycle parameters. Considering that it could be due to rapid degradation or binding of GA to a component of the medium, and/or a rapid metabolism, we measured the GA content both in the Caco2 cells and in the medium after 0, 10, 30, 60 min and 24 h of incubation. Table 3Table 3GA in medium after different incubation times shows a 90% reduction of GA in the medium within 24 h of incubation. Similarly, Figure 4Figure 4


Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2).

Salucci M, Stivala LA, Maiani G, Bugianesi R, Vannini V - Br. J. Cancer (2002)

Time course of cellular uptake of GA on Caco2 cells. Cells were incubated with 100 μM of GA for 10, 30, 60 min and 24 h, and the cellular uptake was evaluated by HPLC. Three independent experiments were made. Bars indicate standard deviation of the mean.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2746583&req=5

fig4: Time course of cellular uptake of GA on Caco2 cells. Cells were incubated with 100 μM of GA for 10, 30, 60 min and 24 h, and the cellular uptake was evaluated by HPLC. Three independent experiments were made. Bars indicate standard deviation of the mean.
Mentions: GA was not detectable, both in the medium and in the cells, after 24, 48 and 72 h of incubation. This result was perplexing as GA exerts a clear effect on cell cycle parameters. Considering that it could be due to rapid degradation or binding of GA to a component of the medium, and/or a rapid metabolism, we measured the GA content both in the Caco2 cells and in the medium after 0, 10, 30, 60 min and 24 h of incubation. Table 3Table 3GA in medium after different incubation times shows a 90% reduction of GA in the medium within 24 h of incubation. Similarly, Figure 4Figure 4

Bottom Line: Quercetin, the major flavonoid present in vegetables and fruit, exerts potential anticarcinogenic effects in animal models and cell cultures, but less is known about quercetin glucosides.The results of this study indicate that all dietary flavonoids studied (epicatechin, epigallocatechin gallate, gallic acid and quercetin-3-glucoside) show a significant antioxidant effect in a chemical model system, but only epigallocatechin gallate or gallic acid are able to interfere with the cell cycle in Caco2 cell lines.From a structural point of view, the galloyl moiety appears to be required for both the antioxidant and the antiproliferative effects.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Food and Nutrition Research, Via Ardeatina 546, 00178, Rome, Italy. salucci@inran.it

Show MeSH
Related in: MedlinePlus