Anti-angiogenic action of hyperthermia by suppressing gene expression and production of tumour-derived vascular endothelial growth factor in vivo and in vitro.
Bottom Line: On the other hand, HT-1080 cell-conditioned medium showed vascular endothelial growth factor-dependent cell proliferative activity and the augmentation of pro-matrix metalloproteinase-1 production in human umbilical vein endothelial cells.The augmentation of endothelial-cell proliferation and pro-matrix metalloproteinase-1 production was poor when human umbilical vein endothelial cells were treated with conditioned medium from heat-shocked HT-1080 cells.These results suggest that hyperthermia acts as an anti-angiogenic strategy by suppressing the expression of tumour-derived vascular endothelial growth factor production and thereby inhibiting endothelial-cell proliferation and extracellular matrix remodelling in blood vessels.
Affiliation: Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.Show MeSH
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Mentions: VEGF possesses the mitogenic activity to cause proliferation of endothelial cells in angiogenesis (Leung et al, 1989; Connolly et al, 1989; Ferrara et al, 1992). We next examined the endothelial-cell proliferative activity of conditioned medium from untreated and heat-shocked HT-1080 cells. When the conditioned medium from HT-1080 cells was added to HUVECs, the endothelial-cell proliferation was increased in a time-dependent manner and doubled for 3 days (Figure 5Figure 5
Affiliation: Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.