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Anti-angiogenic action of hyperthermia by suppressing gene expression and production of tumour-derived vascular endothelial growth factor in vivo and in vitro.

Sawaji Y, Sato T, Takeuchi A, Hirata M, Ito A - Br. J. Cancer (2002)

Bottom Line: The gene expression of alternative splicing variants for vascular endothelial growth factor, VEGF121, VEGF165 and VEGF189, was constitutively detected in HT-1080 cells, but the VEGF189 transcript was less abundant than VEGF121 and VEGF165.On the other hand, HT-1080 cell-conditioned medium showed vascular endothelial growth factor-dependent cell proliferative activity and the augmentation of pro-matrix metalloproteinase-1 production in human umbilical vein endothelial cells.The augmentation of endothelial-cell proliferation and pro-matrix metalloproteinase-1 production was poor when human umbilical vein endothelial cells were treated with conditioned medium from heat-shocked HT-1080 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

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Characterisation of gene expression of VEGF splicing variants in human fibrosarcoma HT-1080 cells. Isolated RNA (1 μg) was subjected to RT–PCR analysis with 25 (lanes 1, 4 and 7), 27 (lanes 2, 5 and 8) and 29 cycles (lanes 3, 6 and 9) using specific primers for respective VEGF splicing variants; VEGF121, VEGF165 and VEGF189 as indicated in Figure 1 and Table 1. Two independent experiments were reproducible and typical data were shown. Lanes 1–3, VEGF121; lanes 4–6, VEGF165 and lanes 7–9, VEGF189.
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fig2: Characterisation of gene expression of VEGF splicing variants in human fibrosarcoma HT-1080 cells. Isolated RNA (1 μg) was subjected to RT–PCR analysis with 25 (lanes 1, 4 and 7), 27 (lanes 2, 5 and 8) and 29 cycles (lanes 3, 6 and 9) using specific primers for respective VEGF splicing variants; VEGF121, VEGF165 and VEGF189 as indicated in Figure 1 and Table 1. Two independent experiments were reproducible and typical data were shown. Lanes 1–3, VEGF121; lanes 4–6, VEGF165 and lanes 7–9, VEGF189.

Mentions: Structure of human VEGF mRNA. Exons are represented by box and numbered. Arrows indicate the specific primers for VEGF variants as shown in Table 1.


Anti-angiogenic action of hyperthermia by suppressing gene expression and production of tumour-derived vascular endothelial growth factor in vivo and in vitro.

Sawaji Y, Sato T, Takeuchi A, Hirata M, Ito A - Br. J. Cancer (2002)

Characterisation of gene expression of VEGF splicing variants in human fibrosarcoma HT-1080 cells. Isolated RNA (1 μg) was subjected to RT–PCR analysis with 25 (lanes 1, 4 and 7), 27 (lanes 2, 5 and 8) and 29 cycles (lanes 3, 6 and 9) using specific primers for respective VEGF splicing variants; VEGF121, VEGF165 and VEGF189 as indicated in Figure 1 and Table 1. Two independent experiments were reproducible and typical data were shown. Lanes 1–3, VEGF121; lanes 4–6, VEGF165 and lanes 7–9, VEGF189.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746582&req=5

fig2: Characterisation of gene expression of VEGF splicing variants in human fibrosarcoma HT-1080 cells. Isolated RNA (1 μg) was subjected to RT–PCR analysis with 25 (lanes 1, 4 and 7), 27 (lanes 2, 5 and 8) and 29 cycles (lanes 3, 6 and 9) using specific primers for respective VEGF splicing variants; VEGF121, VEGF165 and VEGF189 as indicated in Figure 1 and Table 1. Two independent experiments were reproducible and typical data were shown. Lanes 1–3, VEGF121; lanes 4–6, VEGF165 and lanes 7–9, VEGF189.
Mentions: Structure of human VEGF mRNA. Exons are represented by box and numbered. Arrows indicate the specific primers for VEGF variants as shown in Table 1.

Bottom Line: The gene expression of alternative splicing variants for vascular endothelial growth factor, VEGF121, VEGF165 and VEGF189, was constitutively detected in HT-1080 cells, but the VEGF189 transcript was less abundant than VEGF121 and VEGF165.On the other hand, HT-1080 cell-conditioned medium showed vascular endothelial growth factor-dependent cell proliferative activity and the augmentation of pro-matrix metalloproteinase-1 production in human umbilical vein endothelial cells.The augmentation of endothelial-cell proliferation and pro-matrix metalloproteinase-1 production was poor when human umbilical vein endothelial cells were treated with conditioned medium from heat-shocked HT-1080 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Show MeSH
Related in: MedlinePlus