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Anti-angiogenic action of hyperthermia by suppressing gene expression and production of tumour-derived vascular endothelial growth factor in vivo and in vitro.

Sawaji Y, Sato T, Takeuchi A, Hirata M, Ito A - Br. J. Cancer (2002)

Bottom Line: The gene expression of alternative splicing variants for vascular endothelial growth factor, VEGF121, VEGF165 and VEGF189, was constitutively detected in HT-1080 cells, but the VEGF189 transcript was less abundant than VEGF121 and VEGF165.On the other hand, HT-1080 cell-conditioned medium showed vascular endothelial growth factor-dependent cell proliferative activity and the augmentation of pro-matrix metalloproteinase-1 production in human umbilical vein endothelial cells.The augmentation of endothelial-cell proliferation and pro-matrix metalloproteinase-1 production was poor when human umbilical vein endothelial cells were treated with conditioned medium from heat-shocked HT-1080 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

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Structure of human VEGF mRNA. Exons are represented by box and numbered. Arrows indicate the specific primers for VEGF variants as shown in Table 1.
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fig1: Structure of human VEGF mRNA. Exons are represented by box and numbered. Arrows indicate the specific primers for VEGF variants as shown in Table 1.

Mentions: The VEGF gene consists of the common exons 1–5 and the individually specific exons for the variants: exon 8 for VEGF121, exons 7 and 8 for VEGF165, and exons 6, 7 and 8 for VEGF189 (Figure 1Figure 1


Anti-angiogenic action of hyperthermia by suppressing gene expression and production of tumour-derived vascular endothelial growth factor in vivo and in vitro.

Sawaji Y, Sato T, Takeuchi A, Hirata M, Ito A - Br. J. Cancer (2002)

Structure of human VEGF mRNA. Exons are represented by box and numbered. Arrows indicate the specific primers for VEGF variants as shown in Table 1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746582&req=5

fig1: Structure of human VEGF mRNA. Exons are represented by box and numbered. Arrows indicate the specific primers for VEGF variants as shown in Table 1.
Mentions: The VEGF gene consists of the common exons 1–5 and the individually specific exons for the variants: exon 8 for VEGF121, exons 7 and 8 for VEGF165, and exons 6, 7 and 8 for VEGF189 (Figure 1Figure 1

Bottom Line: The gene expression of alternative splicing variants for vascular endothelial growth factor, VEGF121, VEGF165 and VEGF189, was constitutively detected in HT-1080 cells, but the VEGF189 transcript was less abundant than VEGF121 and VEGF165.On the other hand, HT-1080 cell-conditioned medium showed vascular endothelial growth factor-dependent cell proliferative activity and the augmentation of pro-matrix metalloproteinase-1 production in human umbilical vein endothelial cells.The augmentation of endothelial-cell proliferation and pro-matrix metalloproteinase-1 production was poor when human umbilical vein endothelial cells were treated with conditioned medium from heat-shocked HT-1080 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Show MeSH
Related in: MedlinePlus