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Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo.

Jiao W, Miyazaki K, Kitajima Y - Br. J. Cancer (2002)

Bottom Line: However, the molecular mechanism of carcinogenesis and tumour progression remains unclear.These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells.Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.

ABSTRACT
Hepatocellular carcinoma is a well-known malignancy in the world. However, the molecular mechanism of carcinogenesis and tumour progression remains unclear. Recently, reduced E-cadherin expression due to transcriptional suppressor Snail was proven in a panel of epithelial and dedifferentiated cells derived from carcinomas of various etiologies. In the present study, we examined Snail and E-cadherin mRNA/protein expression in five hepatocellular carcinoma cell lines with variable phenotypes (HuL-1, Hep-G(2), Changliver, HLE, and HLF). The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections. By contrast, E-cadherin mRNA and protein expression were only detected in Hep-G(2) cells by RT-PCR and Western blot, respectively. These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells. Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

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IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.
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fig3: IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.

Mentions: By IHC staining of tumour sections, we found membranous expression of endogenous E-cad protein only in Hep-G2 cells (Figure 3BFigure 3


Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo.

Jiao W, Miyazaki K, Kitajima Y - Br. J. Cancer (2002)

IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746537&req=5

fig3: IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.
Mentions: By IHC staining of tumour sections, we found membranous expression of endogenous E-cad protein only in Hep-G2 cells (Figure 3BFigure 3

Bottom Line: However, the molecular mechanism of carcinogenesis and tumour progression remains unclear.These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells.Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.

ABSTRACT
Hepatocellular carcinoma is a well-known malignancy in the world. However, the molecular mechanism of carcinogenesis and tumour progression remains unclear. Recently, reduced E-cadherin expression due to transcriptional suppressor Snail was proven in a panel of epithelial and dedifferentiated cells derived from carcinomas of various etiologies. In the present study, we examined Snail and E-cadherin mRNA/protein expression in five hepatocellular carcinoma cell lines with variable phenotypes (HuL-1, Hep-G(2), Changliver, HLE, and HLF). The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections. By contrast, E-cadherin mRNA and protein expression were only detected in Hep-G(2) cells by RT-PCR and Western blot, respectively. These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells. Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

Show MeSH
Related in: MedlinePlus