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Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo.

Jiao W, Miyazaki K, Kitajima Y - Br. J. Cancer (2002)

Bottom Line: The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections.These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells.Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.

ABSTRACT
Hepatocellular carcinoma is a well-known malignancy in the world. However, the molecular mechanism of carcinogenesis and tumour progression remains unclear. Recently, reduced E-cadherin expression due to transcriptional suppressor Snail was proven in a panel of epithelial and dedifferentiated cells derived from carcinomas of various etiologies. In the present study, we examined Snail and E-cadherin mRNA/protein expression in five hepatocellular carcinoma cell lines with variable phenotypes (HuL-1, Hep-G(2), Changliver, HLE, and HLF). The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections. By contrast, E-cadherin mRNA and protein expression were only detected in Hep-G(2) cells by RT-PCR and Western blot, respectively. These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells. Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

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IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.
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fig3: IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.

Mentions: By IHC staining of tumour sections, we found membranous expression of endogenous E-cad protein only in Hep-G2 cells (Figure 3BFigure 3


Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo.

Jiao W, Miyazaki K, Kitajima Y - Br. J. Cancer (2002)

IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746537&req=5

fig3: IHC staining of endogenous E-cad protein expression in the tumour sections induced by HuL-1 (A), Hep-G2 (B), Changliver (C) and HLF (D) cells (upper panels). Only Hep-G2 tumours clearly showed membranous expression of E-cad. ISH with biotinylated Snail probe detected positive brown staining in the cytoplasm of HuL-1 (E), Changliver (G) and HLF (H) tumour sections, representative of endogenous Snail mRNA expression, but not in Hep-G2 tumours (F) at all (middle panels). Lower panels (I, J, K and L) represented negative controls of each respective section for ISH.
Mentions: By IHC staining of tumour sections, we found membranous expression of endogenous E-cad protein only in Hep-G2 cells (Figure 3BFigure 3

Bottom Line: The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections.These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells.Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.

ABSTRACT
Hepatocellular carcinoma is a well-known malignancy in the world. However, the molecular mechanism of carcinogenesis and tumour progression remains unclear. Recently, reduced E-cadherin expression due to transcriptional suppressor Snail was proven in a panel of epithelial and dedifferentiated cells derived from carcinomas of various etiologies. In the present study, we examined Snail and E-cadherin mRNA/protein expression in five hepatocellular carcinoma cell lines with variable phenotypes (HuL-1, Hep-G(2), Changliver, HLE, and HLF). The results demonstrated that the presence of Snail mRNA in HuL-1, Changliver, HLE and HLF cells detected by RT-PCR, which was further proven by in situ hybridization in tumours induced by HuL-1, Changliver, and HLF cells where Snail mRNA signals expressed in each of the sections. By contrast, E-cadherin mRNA and protein expression were only detected in Hep-G(2) cells by RT-PCR and Western blot, respectively. These results were also consistent with the data obtained from in vivo immunohistochemical staining where membranous expression of endogenous E-cadherin protein was revealed only in tumour sections induced by Hep-G(2) cells. Here we are the first to report that there is an inverse correlation between Snail and E-cadherin expression in HCC cells as well.

Show MeSH
Related in: MedlinePlus