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Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status.

Perel Y, Amrein L, Dobremez E, Rivel J, Daniel JY, Landry M - Br. J. Cancer (2002)

Bottom Line: The messengers coding for galanin, Gal-R1 and -R3 were highly expressed in neuroblastoma and their amount dramatically decreased in ganglioneuroma.Double labelling studies showed that galanin was mainly co-expressed with its receptors whatever the differentiation stage.Finally, our results suggest that galanin influences neuroblastoma growth and development as an autocrine/paracrine modulator.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Differentiation and Development Biology, EA DRED 483, University of Bordeaux 2, 146, rue Leo Saignat, 33076 Bordeaux Cedex, France. yves.perel@chu-bordeaux.fr

ABSTRACT
Neuroblastoma and its benign differentiated counterpart, ganglioneuroma, are paediatric neuroblastic tumours arising in the sympathetic nervous system. Their broad spectrum of clinical virulence is mainly related to heterogeneous biologic background and tumour differentiation. Neuroblastic tumours synthesize various neuropeptides acting as neuromodulators. Previous studies suggested that galanin plays a role in sympathetic tissue where it could be involved in differentiation and development. We investigated the expression and distribution of galanin and its three known receptors (Gal-R1, Gal-R2, Gal-R3) in 19 samples of neuroblastic tumours tissue by immunohistochemistry, in situ hybridization and fluorescent-ligand binding. This study provides clear evidence for galanin and galanin receptor expression in human neuroblastic tumours. The messengers coding for galanin, Gal-R1 and -R3 were highly expressed in neuroblastoma and their amount dramatically decreased in ganglioneuroma. In contrast, Gal-R2 levels remained unchanged. Double labelling studies showed that galanin was mainly co-expressed with its receptors whatever the differentiation stage. In neuroblastic tumours, galanin might promote cell-survival or counteract neuronal differentiation through the different signalling pathways mediated by galanin receptors. Finally, our results suggest that galanin influences neuroblastoma growth and development as an autocrine/paracrine modulator. These findings suggest potential critical implications for galanin in neuroblastic tumours development.

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Dark-field micrographs of emulsion-dipped autoradiograms of NT tissue after hybridization with 35S-labelled probes complementary to galanin receptor mRNA. The NB contained a high density of strongly labelled small round-cells (arrowheads) (A,C and E). In GN, a weak Gal-R1 and Gal-R3 mRNA labelling was detected in the few large neuronal cells (arrows) (B,F) whereas a strong Gal-R2 labelling was observed in neuronal cells (arrowheads) (D). Bars indicate 10 μm.
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fig4: Dark-field micrographs of emulsion-dipped autoradiograms of NT tissue after hybridization with 35S-labelled probes complementary to galanin receptor mRNA. The NB contained a high density of strongly labelled small round-cells (arrowheads) (A,C and E). In GN, a weak Gal-R1 and Gal-R3 mRNA labelling was detected in the few large neuronal cells (arrows) (B,F) whereas a strong Gal-R2 labelling was observed in neuronal cells (arrowheads) (D). Bars indicate 10 μm.

Mentions: Transcripts coding for all three galanin receptors investigated in this study were found in NT (Figure 4Figure 4


Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status.

Perel Y, Amrein L, Dobremez E, Rivel J, Daniel JY, Landry M - Br. J. Cancer (2002)

Dark-field micrographs of emulsion-dipped autoradiograms of NT tissue after hybridization with 35S-labelled probes complementary to galanin receptor mRNA. The NB contained a high density of strongly labelled small round-cells (arrowheads) (A,C and E). In GN, a weak Gal-R1 and Gal-R3 mRNA labelling was detected in the few large neuronal cells (arrows) (B,F) whereas a strong Gal-R2 labelling was observed in neuronal cells (arrowheads) (D). Bars indicate 10 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2746536&req=5

fig4: Dark-field micrographs of emulsion-dipped autoradiograms of NT tissue after hybridization with 35S-labelled probes complementary to galanin receptor mRNA. The NB contained a high density of strongly labelled small round-cells (arrowheads) (A,C and E). In GN, a weak Gal-R1 and Gal-R3 mRNA labelling was detected in the few large neuronal cells (arrows) (B,F) whereas a strong Gal-R2 labelling was observed in neuronal cells (arrowheads) (D). Bars indicate 10 μm.
Mentions: Transcripts coding for all three galanin receptors investigated in this study were found in NT (Figure 4Figure 4

Bottom Line: The messengers coding for galanin, Gal-R1 and -R3 were highly expressed in neuroblastoma and their amount dramatically decreased in ganglioneuroma.Double labelling studies showed that galanin was mainly co-expressed with its receptors whatever the differentiation stage.Finally, our results suggest that galanin influences neuroblastoma growth and development as an autocrine/paracrine modulator.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Differentiation and Development Biology, EA DRED 483, University of Bordeaux 2, 146, rue Leo Saignat, 33076 Bordeaux Cedex, France. yves.perel@chu-bordeaux.fr

ABSTRACT
Neuroblastoma and its benign differentiated counterpart, ganglioneuroma, are paediatric neuroblastic tumours arising in the sympathetic nervous system. Their broad spectrum of clinical virulence is mainly related to heterogeneous biologic background and tumour differentiation. Neuroblastic tumours synthesize various neuropeptides acting as neuromodulators. Previous studies suggested that galanin plays a role in sympathetic tissue where it could be involved in differentiation and development. We investigated the expression and distribution of galanin and its three known receptors (Gal-R1, Gal-R2, Gal-R3) in 19 samples of neuroblastic tumours tissue by immunohistochemistry, in situ hybridization and fluorescent-ligand binding. This study provides clear evidence for galanin and galanin receptor expression in human neuroblastic tumours. The messengers coding for galanin, Gal-R1 and -R3 were highly expressed in neuroblastoma and their amount dramatically decreased in ganglioneuroma. In contrast, Gal-R2 levels remained unchanged. Double labelling studies showed that galanin was mainly co-expressed with its receptors whatever the differentiation stage. In neuroblastic tumours, galanin might promote cell-survival or counteract neuronal differentiation through the different signalling pathways mediated by galanin receptors. Finally, our results suggest that galanin influences neuroblastoma growth and development as an autocrine/paracrine modulator. These findings suggest potential critical implications for galanin in neuroblastic tumours development.

Show MeSH
Related in: MedlinePlus