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Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor.

Zamboni A, Gatto P, Cestaro A, Pilati S, Viola R, Mattivi F, Moser C, Velasco R - BMC Genomics (2009)

Bottom Line: At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases.At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.

View Article: PubMed Central - HTML - PubMed

Affiliation: IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I-38010, Italy. anita.zamboni@univr.it

ABSTRACT

Background: In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.

Results: The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.

Conclusions: We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.

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Modulation of secondary metabolism at 2 and 6 h after DIMEB treatment. Modulation (+ or -) of genes encoding enzymes of phenylalanine biosynthesis, general phenylpropanoid metabolism, monolignol, stilbene and anthocyanin pathways are reported within a simplified secondary metabolism scheme. Abbreviations: DHAP synthase, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase; DHQ synthase, 3-dehydroquinate synthase; CM, chorismate mutase; PDT, prephenate dehydratase; PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate-CoA ligase; CAD, cinnamyl alchol dehydrogenase; CCoAOMT, caffeoyl-CoA 3-O-methyltransferase; COMT, caffeic acid O-methyltransferase; CCR, cinnamoyl-CoA reductase; F5H, ferulate-5-hydroxylase; STS, stilbene synthase; CHI, chalcone isomerase; UFGT, flavonoid-3-O-glucosyltransferase.
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Figure 2: Modulation of secondary metabolism at 2 and 6 h after DIMEB treatment. Modulation (+ or -) of genes encoding enzymes of phenylalanine biosynthesis, general phenylpropanoid metabolism, monolignol, stilbene and anthocyanin pathways are reported within a simplified secondary metabolism scheme. Abbreviations: DHAP synthase, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase; DHQ synthase, 3-dehydroquinate synthase; CM, chorismate mutase; PDT, prephenate dehydratase; PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate-CoA ligase; CAD, cinnamyl alchol dehydrogenase; CCoAOMT, caffeoyl-CoA 3-O-methyltransferase; COMT, caffeic acid O-methyltransferase; CCR, cinnamoyl-CoA reductase; F5H, ferulate-5-hydroxylase; STS, stilbene synthase; CHI, chalcone isomerase; UFGT, flavonoid-3-O-glucosyltransferase.

Mentions: Our results indicated that one of the final grapevine cell responses to the DIMEB-elicited signal consists in the modulation of phenolic metabolism, especially stilbene and monolignol biosynthesis (Figure 2).


Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor.

Zamboni A, Gatto P, Cestaro A, Pilati S, Viola R, Mattivi F, Moser C, Velasco R - BMC Genomics (2009)

Modulation of secondary metabolism at 2 and 6 h after DIMEB treatment. Modulation (+ or -) of genes encoding enzymes of phenylalanine biosynthesis, general phenylpropanoid metabolism, monolignol, stilbene and anthocyanin pathways are reported within a simplified secondary metabolism scheme. Abbreviations: DHAP synthase, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase; DHQ synthase, 3-dehydroquinate synthase; CM, chorismate mutase; PDT, prephenate dehydratase; PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate-CoA ligase; CAD, cinnamyl alchol dehydrogenase; CCoAOMT, caffeoyl-CoA 3-O-methyltransferase; COMT, caffeic acid O-methyltransferase; CCR, cinnamoyl-CoA reductase; F5H, ferulate-5-hydroxylase; STS, stilbene synthase; CHI, chalcone isomerase; UFGT, flavonoid-3-O-glucosyltransferase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2743712&req=5

Figure 2: Modulation of secondary metabolism at 2 and 6 h after DIMEB treatment. Modulation (+ or -) of genes encoding enzymes of phenylalanine biosynthesis, general phenylpropanoid metabolism, monolignol, stilbene and anthocyanin pathways are reported within a simplified secondary metabolism scheme. Abbreviations: DHAP synthase, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase; DHQ synthase, 3-dehydroquinate synthase; CM, chorismate mutase; PDT, prephenate dehydratase; PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate-CoA ligase; CAD, cinnamyl alchol dehydrogenase; CCoAOMT, caffeoyl-CoA 3-O-methyltransferase; COMT, caffeic acid O-methyltransferase; CCR, cinnamoyl-CoA reductase; F5H, ferulate-5-hydroxylase; STS, stilbene synthase; CHI, chalcone isomerase; UFGT, flavonoid-3-O-glucosyltransferase.
Mentions: Our results indicated that one of the final grapevine cell responses to the DIMEB-elicited signal consists in the modulation of phenolic metabolism, especially stilbene and monolignol biosynthesis (Figure 2).

Bottom Line: At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases.At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.

View Article: PubMed Central - HTML - PubMed

Affiliation: IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I-38010, Italy. anita.zamboni@univr.it

ABSTRACT

Background: In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.

Results: The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.

Conclusions: We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.

Show MeSH
Related in: MedlinePlus