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siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication.

Kok KH, Lei T, Jin DY - Retrovirology (2009)

Bottom Line: A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication.This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved, and they could promise to unravel new pathways and new facets of virus-cell interactions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, PR China. khkok@hkucc.hku.hk

ABSTRACT
A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication. This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved, and they could promise to unravel new pathways and new facets of virus-cell interactions.

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A comparison between siRNA- and shRNA-based screens for HIV-1 replication cofactors. See text for additional details.
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Figure 1: A comparison between siRNA- and shRNA-based screens for HIV-1 replication cofactors. See text for additional details.

Mentions: The advent of RNAi-based whole-genome screens in mammalian cells provides a new impetus to the search of host cell factors needed for HIV replication [1,2]. Three screens that used siRNA pools to identify cellular proteins important in HIV-1 replication were reported in 2008, and a meta-analysis of these studies has been published recently [3-6]. One shortcoming to these reported screens is the use of HeLa or HEK293T cells that are not physiological substrates for infection by HIV-1. In addition, the use of a pseudotyped virus or a mutated strain of HIV-1 also limits the interpretability of some of the results. With this backdrop, a recently published genome-wide shRNA-screening performed in Jurkat T lymphocytes for cellular genes that contribute to HIV-1 replication (Figure 1) advances the field by extending the functional screening for cellular factors from attached epithelial/fibroblast cells to suspension T-cells [7].


siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication.

Kok KH, Lei T, Jin DY - Retrovirology (2009)

A comparison between siRNA- and shRNA-based screens for HIV-1 replication cofactors. See text for additional details.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2743632&req=5

Figure 1: A comparison between siRNA- and shRNA-based screens for HIV-1 replication cofactors. See text for additional details.
Mentions: The advent of RNAi-based whole-genome screens in mammalian cells provides a new impetus to the search of host cell factors needed for HIV replication [1,2]. Three screens that used siRNA pools to identify cellular proteins important in HIV-1 replication were reported in 2008, and a meta-analysis of these studies has been published recently [3-6]. One shortcoming to these reported screens is the use of HeLa or HEK293T cells that are not physiological substrates for infection by HIV-1. In addition, the use of a pseudotyped virus or a mutated strain of HIV-1 also limits the interpretability of some of the results. With this backdrop, a recently published genome-wide shRNA-screening performed in Jurkat T lymphocytes for cellular genes that contribute to HIV-1 replication (Figure 1) advances the field by extending the functional screening for cellular factors from attached epithelial/fibroblast cells to suspension T-cells [7].

Bottom Line: A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication.This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved, and they could promise to unravel new pathways and new facets of virus-cell interactions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, PR China. khkok@hkucc.hku.hk

ABSTRACT
A recent RNAi screen used a genome-wide shRNA library to search for cellular factors required for HIV-1 replication. This work complements three other siRNA-based screening studies and potentially opens the door to the discovery of factors that are important for HIV-1 replication in physiological host cells such as T lymphocytes. shRNA screens can be further improved, and they could promise to unravel new pathways and new facets of virus-cell interactions.

Show MeSH