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Neurokinin-1 receptor immunoreactive neuronal elements in the superficial dorsal horn of the chicken spinal cord: with special reference to their relationship with the tachykinin-containing central axon terminals in synaptic glomeruli.

Sakamoto H, Kawate T, Li Y, Atsumi S - Acta Histochem Cytochem (2009)

Bottom Line: Some of the NK-1R immunoreactive profiles also expressed GABA immunoreactivities.Among these elements, dendrites and vesicle-containing dendrites made contact with tachykinin-containing central terminals in the synaptic glomeruli.These results indicate that tachykinin-containing central terminals in the chicken spinal cord can modulate second-order neuronal elements in the synaptic glomeruli.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Physical Therapy, Health Science University, 7187, Kodachi, Fuji-Kawaguchiko, Yamanashi 401-0380, Japan. hiroshi@sakamoto.office.to

ABSTRACT
Synaptic glomeruli that involve tachykinin-containing primary afferent central terminals are numerous in lamina II of the chicken spinal cord. Therefore, a certain amount of noxious information is likely to be modulated in these structures in chickens. In this study, we used immunohistochemistry with confocal and electron microscopy to investigate whether neurokinin-1 receptor (NK-1R)-expressing neuronal elements are in contact with the central primary afferent terminals in synaptic glomeruli of the chicken spinal cord. We also investigated which neuronal elements (axon terminals, dendrites, cell bodies) and which neurons in the spinal cord possess NK-1R, and are possibly influenced by tachykinin in the glomeruli. By confocal microscopy, NK-1R immunoreactivities were seen in a variety of neuronal cell bodies, their dendrites and smaller fibers of unknown origin. Some of the NK-1R immunoreactive profiles also expressed GABA immunoreactivities. A close association was observed between the NK-1R-immunoreactive neurons and tachykinin-immunoreactive axonal varicosities. By electron microscopy, NK-1R immunoreactivity was seen in cell bodies, conventional dendrites and vesicle-containing dendrites in laminae I and II. Among these elements, dendrites and vesicle-containing dendrites made contact with tachykinin-containing central terminals in the synaptic glomeruli. These results indicate that tachykinin-containing central terminals in the chicken spinal cord can modulate second-order neuronal elements in the synaptic glomeruli.

No MeSH data available.


Related in: MedlinePlus

Double labeling at the electron microscopic level. Neurokinin-1 receptor (NK-1R) immunoreactivity is shown with dark diaminobentizine (DAB) reaction products, and tachykinin immunoreactivity is shown with immuno-gold particles. Several NK-1R-immunoreactive neuronal elements made contacts with a tachykinin-containing central axon terminal (CT) in A and C. In A, two vesicle-containing dendrites (VCDs) made synaptic contacts and a conventional dendrite (CD) apposed a tachykinin-immunoreactive axon terminal (CT). In B, an NK-1R-immunoreactive vesicle-containing dendrite (VCD) made two different synapses (arrows) with a tachykinin-immunoreactive axon terminal (AT) and a non-immunoreactive axon terminal. In C, two NK-1R-immunoreactive vesicle-containing dendrites (VCDs) and a conventional dendrite (CD) made synaptic contacts (arrows) with a tachykinin-immunoreactive central axon terminal (CT) in a synaptic glomerulus. Bars=0.5 µm (A–C).
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Figure 3: Double labeling at the electron microscopic level. Neurokinin-1 receptor (NK-1R) immunoreactivity is shown with dark diaminobentizine (DAB) reaction products, and tachykinin immunoreactivity is shown with immuno-gold particles. Several NK-1R-immunoreactive neuronal elements made contacts with a tachykinin-containing central axon terminal (CT) in A and C. In A, two vesicle-containing dendrites (VCDs) made synaptic contacts and a conventional dendrite (CD) apposed a tachykinin-immunoreactive axon terminal (CT). In B, an NK-1R-immunoreactive vesicle-containing dendrite (VCD) made two different synapses (arrows) with a tachykinin-immunoreactive axon terminal (AT) and a non-immunoreactive axon terminal. In C, two NK-1R-immunoreactive vesicle-containing dendrites (VCDs) and a conventional dendrite (CD) made synaptic contacts (arrows) with a tachykinin-immunoreactive central axon terminal (CT) in a synaptic glomerulus. Bars=0.5 µm (A–C).

Mentions: In synaptic structures in lamina II, conventional dendrites (indicated as CD in Fig. 2C) and vesicle-containing dendrites (VCD in Figs. 2D, 3B and 3C) showed NK-1R immunoreactivities. Some of the NK-1R-immunoreactive dendrites and vesicle-containing dendrites consisted of synaptic glomeruli, which have been commonly observed in lamina II of the chicken spinal cord [26]. In NK-1R-immunoreactive neuronal elements, DAB reaction products were seen on all membranous structures, and all post-synaptic densities seen were darkened with the reaction products (Fig. 2D).


Neurokinin-1 receptor immunoreactive neuronal elements in the superficial dorsal horn of the chicken spinal cord: with special reference to their relationship with the tachykinin-containing central axon terminals in synaptic glomeruli.

Sakamoto H, Kawate T, Li Y, Atsumi S - Acta Histochem Cytochem (2009)

Double labeling at the electron microscopic level. Neurokinin-1 receptor (NK-1R) immunoreactivity is shown with dark diaminobentizine (DAB) reaction products, and tachykinin immunoreactivity is shown with immuno-gold particles. Several NK-1R-immunoreactive neuronal elements made contacts with a tachykinin-containing central axon terminal (CT) in A and C. In A, two vesicle-containing dendrites (VCDs) made synaptic contacts and a conventional dendrite (CD) apposed a tachykinin-immunoreactive axon terminal (CT). In B, an NK-1R-immunoreactive vesicle-containing dendrite (VCD) made two different synapses (arrows) with a tachykinin-immunoreactive axon terminal (AT) and a non-immunoreactive axon terminal. In C, two NK-1R-immunoreactive vesicle-containing dendrites (VCDs) and a conventional dendrite (CD) made synaptic contacts (arrows) with a tachykinin-immunoreactive central axon terminal (CT) in a synaptic glomerulus. Bars=0.5 µm (A–C).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2742721&req=5

Figure 3: Double labeling at the electron microscopic level. Neurokinin-1 receptor (NK-1R) immunoreactivity is shown with dark diaminobentizine (DAB) reaction products, and tachykinin immunoreactivity is shown with immuno-gold particles. Several NK-1R-immunoreactive neuronal elements made contacts with a tachykinin-containing central axon terminal (CT) in A and C. In A, two vesicle-containing dendrites (VCDs) made synaptic contacts and a conventional dendrite (CD) apposed a tachykinin-immunoreactive axon terminal (CT). In B, an NK-1R-immunoreactive vesicle-containing dendrite (VCD) made two different synapses (arrows) with a tachykinin-immunoreactive axon terminal (AT) and a non-immunoreactive axon terminal. In C, two NK-1R-immunoreactive vesicle-containing dendrites (VCDs) and a conventional dendrite (CD) made synaptic contacts (arrows) with a tachykinin-immunoreactive central axon terminal (CT) in a synaptic glomerulus. Bars=0.5 µm (A–C).
Mentions: In synaptic structures in lamina II, conventional dendrites (indicated as CD in Fig. 2C) and vesicle-containing dendrites (VCD in Figs. 2D, 3B and 3C) showed NK-1R immunoreactivities. Some of the NK-1R-immunoreactive dendrites and vesicle-containing dendrites consisted of synaptic glomeruli, which have been commonly observed in lamina II of the chicken spinal cord [26]. In NK-1R-immunoreactive neuronal elements, DAB reaction products were seen on all membranous structures, and all post-synaptic densities seen were darkened with the reaction products (Fig. 2D).

Bottom Line: Some of the NK-1R immunoreactive profiles also expressed GABA immunoreactivities.Among these elements, dendrites and vesicle-containing dendrites made contact with tachykinin-containing central terminals in the synaptic glomeruli.These results indicate that tachykinin-containing central terminals in the chicken spinal cord can modulate second-order neuronal elements in the synaptic glomeruli.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Physical Therapy, Health Science University, 7187, Kodachi, Fuji-Kawaguchiko, Yamanashi 401-0380, Japan. hiroshi@sakamoto.office.to

ABSTRACT
Synaptic glomeruli that involve tachykinin-containing primary afferent central terminals are numerous in lamina II of the chicken spinal cord. Therefore, a certain amount of noxious information is likely to be modulated in these structures in chickens. In this study, we used immunohistochemistry with confocal and electron microscopy to investigate whether neurokinin-1 receptor (NK-1R)-expressing neuronal elements are in contact with the central primary afferent terminals in synaptic glomeruli of the chicken spinal cord. We also investigated which neuronal elements (axon terminals, dendrites, cell bodies) and which neurons in the spinal cord possess NK-1R, and are possibly influenced by tachykinin in the glomeruli. By confocal microscopy, NK-1R immunoreactivities were seen in a variety of neuronal cell bodies, their dendrites and smaller fibers of unknown origin. Some of the NK-1R immunoreactive profiles also expressed GABA immunoreactivities. A close association was observed between the NK-1R-immunoreactive neurons and tachykinin-immunoreactive axonal varicosities. By electron microscopy, NK-1R immunoreactivity was seen in cell bodies, conventional dendrites and vesicle-containing dendrites in laminae I and II. Among these elements, dendrites and vesicle-containing dendrites made contact with tachykinin-containing central terminals in the synaptic glomeruli. These results indicate that tachykinin-containing central terminals in the chicken spinal cord can modulate second-order neuronal elements in the synaptic glomeruli.

No MeSH data available.


Related in: MedlinePlus