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Immunohistochemical demonstration of membrane-bound prostaglandin E2 synthase-1 in papillary thyroid carcinoma.

Omi Y, Shibata N, Okamoto T, Obara T, Kobayashi M - Acta Histochem Cytochem (2009)

Bottom Line: However, implications for mPGES-1 in thyroid carcinomas remain to be determined.Staining was more intense in regions with papillary arrangement, while it was less intense in regions with trabecular or solid arrangement.Taken together, our results suggest the involvement of mPGES-1 in proliferation and differentiation of PTC as well as local invasion of PTC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan. yomi@research.twmu.ac.jp

ABSTRACT
Microsomal prostaglandin E(2) synthase-1 (mPGES-1) is an inducible enzyme that catalyzes the conversion of prostaglandin (PG) H(2) to PGE(2) in downstream of cyclooxygenase-2 (COX-2). Recent studies have obtained in vitro evidence that PGE(2) participates in carcinogenesis, angiogenesis, and induction of matrix metalloproteinase-9 (MMP-9), which plays a crucial role in cancer invasion. However, implications for mPGES-1 in thyroid carcinomas remain to be determined. To address this issue, we performed an immunohistochemical analysis for mPGES-1, COX-2 and MMP-9 in 20 papillary thyroid carcinoma (PTC) patients. mPGES-1 immunoreactivity was localized in the cytoplasm of carcinoma cells in 19 cases, with an intensity that tended to be distinct at the interface between the tumor and the surrounding non-neoplastic tissue. Staining was more intense in regions with papillary arrangement, while it was less intense in regions with trabecular or solid arrangement. In many cases, immunohistochemical localization of COX-2 and MMP-9 resemble that of mPGES-1. Taken together, our results suggest the involvement of mPGES-1 in proliferation and differentiation of PTC as well as local invasion of PTC.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of PTC tissue sections immunostained for COX-2 (A, D, G), mPGES-1 (B, E, H) and MMP-9 (C, F, I). Series of panels (A–C), (D–F) and (G–I) indicate the same regions including the interface between PTC tissue (each right half) and non-neoplastic thyroid parenchyma (each left half) on consecutive sections, respectively. Bars=1 mm (A–C), 500 µm (D–F), 100 µm (G–I). ABC method using DAB. ABC, avidin-biotin-immunoperoxidase complex; DAB, 3,3'-diaminobenzidine tetrahydrochloride; PTC, papillary thyroid carcinoma.
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Figure 2: Photomicrographs of PTC tissue sections immunostained for COX-2 (A, D, G), mPGES-1 (B, E, H) and MMP-9 (C, F, I). Series of panels (A–C), (D–F) and (G–I) indicate the same regions including the interface between PTC tissue (each right half) and non-neoplastic thyroid parenchyma (each left half) on consecutive sections, respectively. Bars=1 mm (A–C), 500 µm (D–F), 100 µm (G–I). ABC method using DAB. ABC, avidin-biotin-immunoperoxidase complex; DAB, 3,3'-diaminobenzidine tetrahydrochloride; PTC, papillary thyroid carcinoma.

Mentions: Of the 20 PTC cases, 19 showed focal strong mPGES-1 immunoreactivity, while the other one showed diffuse weak immunoreactivity. The immunoreactivity was localized in the cytoplasm of carcinoma cells in all of the PTC cases, and was prominent at the interface between the tumor and the surrounding non-neoplastic parenchyma (Fig. 2B, E, H). Staining was more intense in regions displaying stromal invasion with papillary arrangement (Fig. 3A), and by contrast it was less intense in regions displaying trabecular arrangement (Fig. 3B) and solid nest formation (Fig. 3C). In many cases, immunohistochemical localization of COX-2 (Fig. 2A, D, G) and MMP-9 (Fig. 2C, F, I) resembled that of mPGES-1. However, immunoreactivities for COX-2 and MMP-9 were diffuse and uniform in PTC cells of 13 cases and four cases, respectively; of the latter four cases, the mPGES-1 immunoreactivity was weak in one case and strong in three cases. Of the 20 PTC cases, seven were with CT, and the rest were not associated with any other thyroid disease. In the seven CT cases, non-neoplastic thyroid parenchyma demonstrated scattered formation of lymph follicles and swelling of follicular epithelial cells that often had eosinophilic cytoplasm and showed papillary arrangement. Immunoreactivities for mPGES-1 and MMP-9 were detected in germinal center lymphocytes and papillary-arranged follicular epithelial cells in five cases (Fig. 4A–C), but were undetectable in intact follicular epithelial cells in all of the CT cases. Immunohistochemical localization of COX-2 was similar to that of mPGES-1 and MMP-9 in three CT cases (Fig. 4A–C). Normal thyroid parenchyma did not show any marked staining for mPGES-1, COX-2 or MMP-9 (Fig. 4A–C).


Immunohistochemical demonstration of membrane-bound prostaglandin E2 synthase-1 in papillary thyroid carcinoma.

Omi Y, Shibata N, Okamoto T, Obara T, Kobayashi M - Acta Histochem Cytochem (2009)

Photomicrographs of PTC tissue sections immunostained for COX-2 (A, D, G), mPGES-1 (B, E, H) and MMP-9 (C, F, I). Series of panels (A–C), (D–F) and (G–I) indicate the same regions including the interface between PTC tissue (each right half) and non-neoplastic thyroid parenchyma (each left half) on consecutive sections, respectively. Bars=1 mm (A–C), 500 µm (D–F), 100 µm (G–I). ABC method using DAB. ABC, avidin-biotin-immunoperoxidase complex; DAB, 3,3'-diaminobenzidine tetrahydrochloride; PTC, papillary thyroid carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2742720&req=5

Figure 2: Photomicrographs of PTC tissue sections immunostained for COX-2 (A, D, G), mPGES-1 (B, E, H) and MMP-9 (C, F, I). Series of panels (A–C), (D–F) and (G–I) indicate the same regions including the interface between PTC tissue (each right half) and non-neoplastic thyroid parenchyma (each left half) on consecutive sections, respectively. Bars=1 mm (A–C), 500 µm (D–F), 100 µm (G–I). ABC method using DAB. ABC, avidin-biotin-immunoperoxidase complex; DAB, 3,3'-diaminobenzidine tetrahydrochloride; PTC, papillary thyroid carcinoma.
Mentions: Of the 20 PTC cases, 19 showed focal strong mPGES-1 immunoreactivity, while the other one showed diffuse weak immunoreactivity. The immunoreactivity was localized in the cytoplasm of carcinoma cells in all of the PTC cases, and was prominent at the interface between the tumor and the surrounding non-neoplastic parenchyma (Fig. 2B, E, H). Staining was more intense in regions displaying stromal invasion with papillary arrangement (Fig. 3A), and by contrast it was less intense in regions displaying trabecular arrangement (Fig. 3B) and solid nest formation (Fig. 3C). In many cases, immunohistochemical localization of COX-2 (Fig. 2A, D, G) and MMP-9 (Fig. 2C, F, I) resembled that of mPGES-1. However, immunoreactivities for COX-2 and MMP-9 were diffuse and uniform in PTC cells of 13 cases and four cases, respectively; of the latter four cases, the mPGES-1 immunoreactivity was weak in one case and strong in three cases. Of the 20 PTC cases, seven were with CT, and the rest were not associated with any other thyroid disease. In the seven CT cases, non-neoplastic thyroid parenchyma demonstrated scattered formation of lymph follicles and swelling of follicular epithelial cells that often had eosinophilic cytoplasm and showed papillary arrangement. Immunoreactivities for mPGES-1 and MMP-9 were detected in germinal center lymphocytes and papillary-arranged follicular epithelial cells in five cases (Fig. 4A–C), but were undetectable in intact follicular epithelial cells in all of the CT cases. Immunohistochemical localization of COX-2 was similar to that of mPGES-1 and MMP-9 in three CT cases (Fig. 4A–C). Normal thyroid parenchyma did not show any marked staining for mPGES-1, COX-2 or MMP-9 (Fig. 4A–C).

Bottom Line: However, implications for mPGES-1 in thyroid carcinomas remain to be determined.Staining was more intense in regions with papillary arrangement, while it was less intense in regions with trabecular or solid arrangement.Taken together, our results suggest the involvement of mPGES-1 in proliferation and differentiation of PTC as well as local invasion of PTC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan. yomi@research.twmu.ac.jp

ABSTRACT
Microsomal prostaglandin E(2) synthase-1 (mPGES-1) is an inducible enzyme that catalyzes the conversion of prostaglandin (PG) H(2) to PGE(2) in downstream of cyclooxygenase-2 (COX-2). Recent studies have obtained in vitro evidence that PGE(2) participates in carcinogenesis, angiogenesis, and induction of matrix metalloproteinase-9 (MMP-9), which plays a crucial role in cancer invasion. However, implications for mPGES-1 in thyroid carcinomas remain to be determined. To address this issue, we performed an immunohistochemical analysis for mPGES-1, COX-2 and MMP-9 in 20 papillary thyroid carcinoma (PTC) patients. mPGES-1 immunoreactivity was localized in the cytoplasm of carcinoma cells in 19 cases, with an intensity that tended to be distinct at the interface between the tumor and the surrounding non-neoplastic tissue. Staining was more intense in regions with papillary arrangement, while it was less intense in regions with trabecular or solid arrangement. In many cases, immunohistochemical localization of COX-2 and MMP-9 resemble that of mPGES-1. Taken together, our results suggest the involvement of mPGES-1 in proliferation and differentiation of PTC as well as local invasion of PTC.

No MeSH data available.


Related in: MedlinePlus